<i>Angiopteris cochinchinensis</i> de Vriese Ameliorates LPS-Induced Acute Lung Injury via Src Inhibition
Growing demand for treatment options against acute lung injury (ALI) emphasizes studies on plant extracts harboring anti-inflammatory effects. According to GC-MS analysis, <i>Angiopteris cochinchinensis</i> de Vriese consists of various flavonoids with anti-inflammatory activities. Thus,...
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| Format: | Article |
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MDPI AG
2022-05-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/10/1306 |
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| author | Won Young Jang Hwa Pyoung Lee Seung A Kim Lei Huang Ji Hye Yoon Chae Yun Shin Ankita Mitra Han Gyung Kim Jae Youl Cho |
| author_facet | Won Young Jang Hwa Pyoung Lee Seung A Kim Lei Huang Ji Hye Yoon Chae Yun Shin Ankita Mitra Han Gyung Kim Jae Youl Cho |
| author_sort | Won Young Jang |
| collection | DOAJ |
| description | Growing demand for treatment options against acute lung injury (ALI) emphasizes studies on plant extracts harboring anti-inflammatory effects. According to GC-MS analysis, <i>Angiopteris cochinchinensis</i> de Vriese consists of various flavonoids with anti-inflammatory activities. Thus, in this study, the anti-inflammatory effects of an extract of <i>Angiopteris cochinchinensis</i> de Vriese (Ac-EE) were assessed using RAW264.6 murine macrophages and a lipopolysaccharide (LPS)-induced ALI model. Ac-EE reduced the nitric oxide production in murine macrophages increased by LPS induction. Moreover, protective effects of Ac-EE on lung tissue were demonstrated by shrinkage of edema and lung injury. Reduced neutrophil infiltration and formation of hyaline membranes were also detected in lung tissues after H&E staining. Semiquantitative RT-PCR, quantitative real-time PCR, and ELISA showed that Ac-EE inhibits the production of proinflammatory mediators, including <i>i</i>NOS and COX-2, and cytokines, such as TNF-α, IL-1β, and IL-6. An Ac-EE-mediated anti-inflammatory response was derived from inhibiting the NF-κB signaling pathway, which was evaluated by luciferase reporter assay and Western blotting analysis. A cellular thermal shift assay revealed that the prime target of Ac-EE in alleviating inflammation was Src. With its direct binding with Src, <i>Angiopteris cochinchinensis</i> de Vriese significantly mitigates lung injury, showing possibilities of its potential as an effective botanical drug. |
| first_indexed | 2024-03-10T03:03:09Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T03:03:09Z |
| publishDate | 2022-05-01 |
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| record_format | Article |
| series | Plants |
| spelling | doaj.art-db4f61d73db048debb503eccdfe8d32b2023-11-23T12:42:01ZengMDPI AGPlants2223-77472022-05-011110130610.3390/plants11101306<i>Angiopteris cochinchinensis</i> de Vriese Ameliorates LPS-Induced Acute Lung Injury via Src InhibitionWon Young Jang0Hwa Pyoung Lee1Seung A Kim2Lei Huang3Ji Hye Yoon4Chae Yun Shin5Ankita Mitra6Han Gyung Kim7Jae Youl Cho8Department of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, KoreaDepartment of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, KoreaDepartment of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, KoreaDepartment of Biocosmetics, Sungkyunkwan University, Suwon 16419, KoreaDepartment of Biocosmetics, Sungkyunkwan University, Suwon 16419, KoreaDepartment of Biocosmetics, Sungkyunkwan University, Suwon 16419, KoreaDepartment of Integrative Biotechnology and Biomedical Institute for Convergence at SKKU (BICS), Sungkyunkwan University, Suwon 16419, KoreaDepartment of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, KoreaDepartment of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, KoreaGrowing demand for treatment options against acute lung injury (ALI) emphasizes studies on plant extracts harboring anti-inflammatory effects. According to GC-MS analysis, <i>Angiopteris cochinchinensis</i> de Vriese consists of various flavonoids with anti-inflammatory activities. Thus, in this study, the anti-inflammatory effects of an extract of <i>Angiopteris cochinchinensis</i> de Vriese (Ac-EE) were assessed using RAW264.6 murine macrophages and a lipopolysaccharide (LPS)-induced ALI model. Ac-EE reduced the nitric oxide production in murine macrophages increased by LPS induction. Moreover, protective effects of Ac-EE on lung tissue were demonstrated by shrinkage of edema and lung injury. Reduced neutrophil infiltration and formation of hyaline membranes were also detected in lung tissues after H&E staining. Semiquantitative RT-PCR, quantitative real-time PCR, and ELISA showed that Ac-EE inhibits the production of proinflammatory mediators, including <i>i</i>NOS and COX-2, and cytokines, such as TNF-α, IL-1β, and IL-6. An Ac-EE-mediated anti-inflammatory response was derived from inhibiting the NF-κB signaling pathway, which was evaluated by luciferase reporter assay and Western blotting analysis. A cellular thermal shift assay revealed that the prime target of Ac-EE in alleviating inflammation was Src. With its direct binding with Src, <i>Angiopteris cochinchinensis</i> de Vriese significantly mitigates lung injury, showing possibilities of its potential as an effective botanical drug.https://www.mdpi.com/2223-7747/11/10/1306<i>Angiopteris cochinchinensis</i> de VrieseLPSacute lung injuryNF-κBSrcanti-inflammation |
| spellingShingle | Won Young Jang Hwa Pyoung Lee Seung A Kim Lei Huang Ji Hye Yoon Chae Yun Shin Ankita Mitra Han Gyung Kim Jae Youl Cho <i>Angiopteris cochinchinensis</i> de Vriese Ameliorates LPS-Induced Acute Lung Injury via Src Inhibition Plants <i>Angiopteris cochinchinensis</i> de Vriese LPS acute lung injury NF-κB Src anti-inflammation |
| title | <i>Angiopteris cochinchinensis</i> de Vriese Ameliorates LPS-Induced Acute Lung Injury via Src Inhibition |
| title_full | <i>Angiopteris cochinchinensis</i> de Vriese Ameliorates LPS-Induced Acute Lung Injury via Src Inhibition |
| title_fullStr | <i>Angiopteris cochinchinensis</i> de Vriese Ameliorates LPS-Induced Acute Lung Injury via Src Inhibition |
| title_full_unstemmed | <i>Angiopteris cochinchinensis</i> de Vriese Ameliorates LPS-Induced Acute Lung Injury via Src Inhibition |
| title_short | <i>Angiopteris cochinchinensis</i> de Vriese Ameliorates LPS-Induced Acute Lung Injury via Src Inhibition |
| title_sort | i angiopteris cochinchinensis i de vriese ameliorates lps induced acute lung injury via src inhibition |
| topic | <i>Angiopteris cochinchinensis</i> de Vriese LPS acute lung injury NF-κB Src anti-inflammation |
| url | https://www.mdpi.com/2223-7747/11/10/1306 |
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