Comparative Transcriptome Analysis of Shewanella putrefaciens WS13 Biofilms Under Cold Stress
Shewanella putrefaciens is a Gram-negative bacterium that can cause seafood spoilage under low-temperature conditions. The bacterium easily forms biofilms to enhance its survival in challenging environments. Our previous research revealed that the biofilm formed by S. putrefaciens WS13 under the low...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.851521/full |
| _version_ | 1828822368726810624 |
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| author | Jun Yan Jun Yan Zhijun Yang Jing Xie Jing Xie Jing Xie Jing Xie |
| author_facet | Jun Yan Jun Yan Zhijun Yang Jing Xie Jing Xie Jing Xie Jing Xie |
| author_sort | Jun Yan |
| collection | DOAJ |
| description | Shewanella putrefaciens is a Gram-negative bacterium that can cause seafood spoilage under low-temperature conditions. The bacterium easily forms biofilms to enhance its survival in challenging environments. Our previous research revealed that the biofilm formed by S. putrefaciens WS13 under the low temperature (4 °C) has larger biomass and tighter structure than at an optimum growth temperature (30 °C). In this study, comparative transcriptome analysis was further performed to get insights into the global-level of gene expression in the biofilm formed by S. putrefaciens WS13 under the refrigerating and optimal temperatures using Illumina RNA-Sequencing technique. The results revealed that a total of 761 genes were differentially expressed, of which 497 were significantly up-regulated and 264 were significantly down-regulated (p<0.05). The qRT-PCR results of randomly selected differentially expressed genes (DEGs) confirmed the RNA sequencing results. Comparison of transcriptome data revealed 28 significantly changed metabolic pathways under the cold stress, including the down-regulated chemotaxis, and motility, and up-regulated tryptophan metabolism, histidine biosynthesis, and quorum sensing, which benefited the biofilm formation of S. putrefaciens WS13 under the adverse circumstance. This study provided useful data for better understanding of the biofilm formation of S. putrefaciens, and also laid a theoretical foundation for novel vaccine and drug targets against the severe spoilage bacterium under the cold stress. |
| first_indexed | 2024-12-12T13:12:28Z |
| format | Article |
| id | doaj.art-db5b80224a4747f990ffdaef7ae4149c |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-12T13:12:28Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-db5b80224a4747f990ffdaef7ae4149c2022-12-22T00:23:29ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-06-011210.3389/fcimb.2022.851521851521Comparative Transcriptome Analysis of Shewanella putrefaciens WS13 Biofilms Under Cold StressJun Yan0Jun Yan1Zhijun Yang2Jing Xie3Jing Xie4Jing Xie5Jing Xie6College of Food Science & Technology, Shanghai Ocean University, Shanghai, ChinaLaboratory for Quality and Safety Risk Assessment of Aquatic Products in Storage and Preservation of Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, ChinaCollege of Food Science & Technology, Shanghai Ocean University, Shanghai, ChinaCollege of Food Science & Technology, Shanghai Ocean University, Shanghai, ChinaLaboratory for Quality and Safety Risk Assessment of Aquatic Products in Storage and Preservation of Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, ChinaShanghai Professional Technology Service Platform on Cold Chain Equipment Performance and Energy Saving Evaluation, Shanghai Ocean University, Shanghai, ChinaNational Experimental Teaching Demonstration Center for Food Science and Engineering, Shanghai Ocean University, Shanghai, ChinaShewanella putrefaciens is a Gram-negative bacterium that can cause seafood spoilage under low-temperature conditions. The bacterium easily forms biofilms to enhance its survival in challenging environments. Our previous research revealed that the biofilm formed by S. putrefaciens WS13 under the low temperature (4 °C) has larger biomass and tighter structure than at an optimum growth temperature (30 °C). In this study, comparative transcriptome analysis was further performed to get insights into the global-level of gene expression in the biofilm formed by S. putrefaciens WS13 under the refrigerating and optimal temperatures using Illumina RNA-Sequencing technique. The results revealed that a total of 761 genes were differentially expressed, of which 497 were significantly up-regulated and 264 were significantly down-regulated (p<0.05). The qRT-PCR results of randomly selected differentially expressed genes (DEGs) confirmed the RNA sequencing results. Comparison of transcriptome data revealed 28 significantly changed metabolic pathways under the cold stress, including the down-regulated chemotaxis, and motility, and up-regulated tryptophan metabolism, histidine biosynthesis, and quorum sensing, which benefited the biofilm formation of S. putrefaciens WS13 under the adverse circumstance. This study provided useful data for better understanding of the biofilm formation of S. putrefaciens, and also laid a theoretical foundation for novel vaccine and drug targets against the severe spoilage bacterium under the cold stress.https://www.frontiersin.org/articles/10.3389/fcimb.2022.851521/fullShewanella putrefaciens WS 13biofilmcold stresstranscriptomespecific spoilage organism |
| spellingShingle | Jun Yan Jun Yan Zhijun Yang Jing Xie Jing Xie Jing Xie Jing Xie Comparative Transcriptome Analysis of Shewanella putrefaciens WS13 Biofilms Under Cold Stress Frontiers in Cellular and Infection Microbiology Shewanella putrefaciens WS 13 biofilm cold stress transcriptome specific spoilage organism |
| title | Comparative Transcriptome Analysis of Shewanella putrefaciens WS13 Biofilms Under Cold Stress |
| title_full | Comparative Transcriptome Analysis of Shewanella putrefaciens WS13 Biofilms Under Cold Stress |
| title_fullStr | Comparative Transcriptome Analysis of Shewanella putrefaciens WS13 Biofilms Under Cold Stress |
| title_full_unstemmed | Comparative Transcriptome Analysis of Shewanella putrefaciens WS13 Biofilms Under Cold Stress |
| title_short | Comparative Transcriptome Analysis of Shewanella putrefaciens WS13 Biofilms Under Cold Stress |
| title_sort | comparative transcriptome analysis of shewanella putrefaciens ws13 biofilms under cold stress |
| topic | Shewanella putrefaciens WS 13 biofilm cold stress transcriptome specific spoilage organism |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.851521/full |
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