Investigating the Short-Term Effects of Cold Stress on Metabolite Responses and Metabolic Pathways in Inner-Mongolia Sanhe Cattle
Inner-Mongolia Sanhe cattle are well-adapted to low-temperature conditions, but the metabolic mechanisms underlying their climatic resilience are still unknown. Based on the <sup>1</sup>H Nuclear Magnetic Resonance platform, 41 metabolites were identified and quantified in the serum of 1...
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| Format: | Article |
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MDPI AG
2021-08-01
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| Online Access: | https://www.mdpi.com/2076-2615/11/9/2493 |
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| author | Lirong Hu Luiz F. Brito Zaheer Abbas Abdul Sammad Ling Kang Dongsheng Wang Hongjun Wu Airong Liu Guiqiang Qi Man Zhao Yachun Wang Qing Xu |
| author_facet | Lirong Hu Luiz F. Brito Zaheer Abbas Abdul Sammad Ling Kang Dongsheng Wang Hongjun Wu Airong Liu Guiqiang Qi Man Zhao Yachun Wang Qing Xu |
| author_sort | Lirong Hu |
| collection | DOAJ |
| description | Inner-Mongolia Sanhe cattle are well-adapted to low-temperature conditions, but the metabolic mechanisms underlying their climatic resilience are still unknown. Based on the <sup>1</sup>H Nuclear Magnetic Resonance platform, 41 metabolites were identified and quantified in the serum of 10 heifers under thermal neutrality (5 °C), and subsequent exposure to hyper-cold temperature (−32 °C) for 3 h. Subsequently, 28 metabolites were pre-filtrated, and they provided better performance in multivariate analysis than that of using 41 metabolites. This indicated the need for pre-filtering of the metabolome data in a paired experimental design. In response to the cold exposure challenge, 19 metabolites associated with cold stress response were identified, mainly enriched in “aminoacyl-tRNA biosynthesis” and “valine, leucine, and isoleucine degradation”. A further integration of metabolome and gene expression highlighted the functional roles of the <i>DLD</i> (dihydrolipoamide dehydrogenase), <i>WARS</i> (tryptophanyl-tRNA synthetase), and <i>RARS</i> (arginyl-tRNA synthetase) genes in metabolic pathways of valine and leucine. Furthermore, the essential regulations of <i>SLC30A6</i> (solute carrier family 30 (zinc transporter), member 6) in metabolic transportation for propionate, acetate, valine, and leucine under severe cold exposure were observed. Our findings presented a comprehensive characterization of the serum metabolome of Inner-Mongolia Sanhe cattle, and contributed to a better understanding of the crucial roles of regulations in metabolites and metabolic pathways during cold stress events in cattle. |
| first_indexed | 2024-03-10T07:58:31Z |
| format | Article |
| id | doaj.art-db614b501c7844708c5ed78b0dc4a7b6 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T07:58:31Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-db614b501c7844708c5ed78b0dc4a7b62023-11-22T11:40:56ZengMDPI AGAnimals2076-26152021-08-01119249310.3390/ani11092493Investigating the Short-Term Effects of Cold Stress on Metabolite Responses and Metabolic Pathways in Inner-Mongolia Sanhe CattleLirong Hu0Luiz F. Brito1Zaheer Abbas2Abdul Sammad3Ling Kang4Dongsheng Wang5Hongjun Wu6Airong Liu7Guiqiang Qi8Man Zhao9Yachun Wang10Qing Xu11College of Life Sciences and Bioengineering, Beijing Jiaotong University, Beijing 100044, ChinaDepartment of Animal Sciences, Purdue University, West Lafayette, IN 47907, USACollege of Life Sciences and Bioengineering, Beijing Jiaotong University, Beijing 100044, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction, MARA, National Engineering Laboratory for Animal Breeding, Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaCollege of Life Sciences and Bioengineering, Beijing Jiaotong University, Beijing 100044, ChinaXiertala Cattle Breeding Farm, Hailaer Farm Buro, Hailaer, Hulunbuir 021012, ChinaXiertala Cattle Breeding Farm, Hailaer Farm Buro, Hailaer, Hulunbuir 021012, ChinaXiertala Cattle Breeding Farm, Hailaer Farm Buro, Hailaer, Hulunbuir 021012, ChinaXiertala Cattle Breeding Farm, Hailaer Farm Buro, Hailaer, Hulunbuir 021012, ChinaXiertala Cattle Breeding Farm, Hailaer Farm Buro, Hailaer, Hulunbuir 021012, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction, MARA, National Engineering Laboratory for Animal Breeding, Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaCollege of Life Sciences and Bioengineering, Beijing Jiaotong University, Beijing 100044, ChinaInner-Mongolia Sanhe cattle are well-adapted to low-temperature conditions, but the metabolic mechanisms underlying their climatic resilience are still unknown. Based on the <sup>1</sup>H Nuclear Magnetic Resonance platform, 41 metabolites were identified and quantified in the serum of 10 heifers under thermal neutrality (5 °C), and subsequent exposure to hyper-cold temperature (−32 °C) for 3 h. Subsequently, 28 metabolites were pre-filtrated, and they provided better performance in multivariate analysis than that of using 41 metabolites. This indicated the need for pre-filtering of the metabolome data in a paired experimental design. In response to the cold exposure challenge, 19 metabolites associated with cold stress response were identified, mainly enriched in “aminoacyl-tRNA biosynthesis” and “valine, leucine, and isoleucine degradation”. A further integration of metabolome and gene expression highlighted the functional roles of the <i>DLD</i> (dihydrolipoamide dehydrogenase), <i>WARS</i> (tryptophanyl-tRNA synthetase), and <i>RARS</i> (arginyl-tRNA synthetase) genes in metabolic pathways of valine and leucine. Furthermore, the essential regulations of <i>SLC30A6</i> (solute carrier family 30 (zinc transporter), member 6) in metabolic transportation for propionate, acetate, valine, and leucine under severe cold exposure were observed. Our findings presented a comprehensive characterization of the serum metabolome of Inner-Mongolia Sanhe cattle, and contributed to a better understanding of the crucial roles of regulations in metabolites and metabolic pathways during cold stress events in cattle.https://www.mdpi.com/2076-2615/11/9/2493cold stressSanhe cattlemetabolomicsclimatic resilience |
| spellingShingle | Lirong Hu Luiz F. Brito Zaheer Abbas Abdul Sammad Ling Kang Dongsheng Wang Hongjun Wu Airong Liu Guiqiang Qi Man Zhao Yachun Wang Qing Xu Investigating the Short-Term Effects of Cold Stress on Metabolite Responses and Metabolic Pathways in Inner-Mongolia Sanhe Cattle Animals cold stress Sanhe cattle metabolomics climatic resilience |
| title | Investigating the Short-Term Effects of Cold Stress on Metabolite Responses and Metabolic Pathways in Inner-Mongolia Sanhe Cattle |
| title_full | Investigating the Short-Term Effects of Cold Stress on Metabolite Responses and Metabolic Pathways in Inner-Mongolia Sanhe Cattle |
| title_fullStr | Investigating the Short-Term Effects of Cold Stress on Metabolite Responses and Metabolic Pathways in Inner-Mongolia Sanhe Cattle |
| title_full_unstemmed | Investigating the Short-Term Effects of Cold Stress on Metabolite Responses and Metabolic Pathways in Inner-Mongolia Sanhe Cattle |
| title_short | Investigating the Short-Term Effects of Cold Stress on Metabolite Responses and Metabolic Pathways in Inner-Mongolia Sanhe Cattle |
| title_sort | investigating the short term effects of cold stress on metabolite responses and metabolic pathways in inner mongolia sanhe cattle |
| topic | cold stress Sanhe cattle metabolomics climatic resilience |
| url | https://www.mdpi.com/2076-2615/11/9/2493 |
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