Assessment of automated assays for serum amyloid A, haptoglobin (PIT54) and basic biochemistry in broiler breeders experimentally infected with Escherichia coli
Abstract Biomarkers of inflammation are valuable tools for health status evaluation in numerous species. However, in poultry, methods for measuring acute phase proteins (APP) are sparse and rely on manual laboratory labour reserving these parameters mainly for research studies with APP as a focus po...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-03-01
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| Series: | Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s13567-022-01040-1 |
| _version_ | 1811331925603778560 |
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| author | Sofie Kromann Rikke Heidemann Olsen Anders Miki Bojesen Henrik Elvang Jensen Ida Thøfner |
| author_facet | Sofie Kromann Rikke Heidemann Olsen Anders Miki Bojesen Henrik Elvang Jensen Ida Thøfner |
| author_sort | Sofie Kromann |
| collection | DOAJ |
| description | Abstract Biomarkers of inflammation are valuable tools for health status evaluation in numerous species. However, in poultry, methods for measuring acute phase proteins (APP) are sparse and rely on manual laboratory labour reserving these parameters mainly for research studies with APP as a focus point. To extend the use of APP beyond tightly focused research studies, blood from experimentally infected and control hens was analysed using equipment available in many veterinary clinics in order to identify easily accessible biomarkers of infection. Blood samples from broiler breeders (n = 30) inoculated intratracheally with either Escherichia coli or sterile vehicle were randomly selected at 2, 4 and 7 days post-infection (dpi) and subjected to biochemical analysis. Samples for bacteriological testing were collected, and all animals were subjected to a full necropsy for disease confirmation. Significantly higher levels of serum amyloid A were evident in the infected birds at 2 and 4 dpi (p < 0.01) compared to the controls. Likewise, haptoglobin (PIT54) levels were significantly elevated at 4 dpi (p < 0.01) in the infected animal, whilst at 2 dpi magnesium and calcium were significantly lower in the infected group (p < 0.05). Gross pathology and bacteriology confirmed the presence of infection in the E. coli inoculated birds. In conclusion, equipment routinely used in other species for rapid analysis of blood samples, successfully differentiated between sick and healthy birds, hereby, showing great potential as an easily added parameter of evaluation in research studies, and as a valuable decision-making tool for poultry veterinarians. |
| first_indexed | 2024-04-13T16:29:05Z |
| format | Article |
| id | doaj.art-db624c950c3748f3a55356ed345009ae |
| institution | Directory Open Access Journal |
| issn | 1297-9716 |
| language | English |
| last_indexed | 2024-04-13T16:29:05Z |
| publishDate | 2022-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj.art-db624c950c3748f3a55356ed345009ae2022-12-22T02:39:40ZengBMCVeterinary Research1297-97162022-03-015311710.1186/s13567-022-01040-1Assessment of automated assays for serum amyloid A, haptoglobin (PIT54) and basic biochemistry in broiler breeders experimentally infected with Escherichia coliSofie Kromann0Rikke Heidemann Olsen1Anders Miki Bojesen2Henrik Elvang Jensen3Ida Thøfner4Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of CopenhagenDepartment of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of CopenhagenDepartment of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of CopenhagenDepartment of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of CopenhagenDepartment of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of CopenhagenAbstract Biomarkers of inflammation are valuable tools for health status evaluation in numerous species. However, in poultry, methods for measuring acute phase proteins (APP) are sparse and rely on manual laboratory labour reserving these parameters mainly for research studies with APP as a focus point. To extend the use of APP beyond tightly focused research studies, blood from experimentally infected and control hens was analysed using equipment available in many veterinary clinics in order to identify easily accessible biomarkers of infection. Blood samples from broiler breeders (n = 30) inoculated intratracheally with either Escherichia coli or sterile vehicle were randomly selected at 2, 4 and 7 days post-infection (dpi) and subjected to biochemical analysis. Samples for bacteriological testing were collected, and all animals were subjected to a full necropsy for disease confirmation. Significantly higher levels of serum amyloid A were evident in the infected birds at 2 and 4 dpi (p < 0.01) compared to the controls. Likewise, haptoglobin (PIT54) levels were significantly elevated at 4 dpi (p < 0.01) in the infected animal, whilst at 2 dpi magnesium and calcium were significantly lower in the infected group (p < 0.05). Gross pathology and bacteriology confirmed the presence of infection in the E. coli inoculated birds. In conclusion, equipment routinely used in other species for rapid analysis of blood samples, successfully differentiated between sick and healthy birds, hereby, showing great potential as an easily added parameter of evaluation in research studies, and as a valuable decision-making tool for poultry veterinarians.https://doi.org/10.1186/s13567-022-01040-1Blood biochemistrybiomarkeracute phase proteinserum amyloid Ahaptoglobin |
| spellingShingle | Sofie Kromann Rikke Heidemann Olsen Anders Miki Bojesen Henrik Elvang Jensen Ida Thøfner Assessment of automated assays for serum amyloid A, haptoglobin (PIT54) and basic biochemistry in broiler breeders experimentally infected with Escherichia coli Veterinary Research Blood biochemistry biomarker acute phase protein serum amyloid A haptoglobin |
| title | Assessment of automated assays for serum amyloid A, haptoglobin (PIT54) and basic biochemistry in broiler breeders experimentally infected with Escherichia coli |
| title_full | Assessment of automated assays for serum amyloid A, haptoglobin (PIT54) and basic biochemistry in broiler breeders experimentally infected with Escherichia coli |
| title_fullStr | Assessment of automated assays for serum amyloid A, haptoglobin (PIT54) and basic biochemistry in broiler breeders experimentally infected with Escherichia coli |
| title_full_unstemmed | Assessment of automated assays for serum amyloid A, haptoglobin (PIT54) and basic biochemistry in broiler breeders experimentally infected with Escherichia coli |
| title_short | Assessment of automated assays for serum amyloid A, haptoglobin (PIT54) and basic biochemistry in broiler breeders experimentally infected with Escherichia coli |
| title_sort | assessment of automated assays for serum amyloid a haptoglobin pit54 and basic biochemistry in broiler breeders experimentally infected with escherichia coli |
| topic | Blood biochemistry biomarker acute phase protein serum amyloid A haptoglobin |
| url | https://doi.org/10.1186/s13567-022-01040-1 |
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