In Vivo Evaluation of PVP-Gelatin-Chitosan Composite Blended with Egg-Yolk Oil for Radiodermatitis
Radiation-induced skin injury (RSI) is a frequent complication of radiotherapy. Severe cases affect the patient’s disease treatment and quality of life. The main purpose of this study is to examine the causative role of egg-yolk oil (EYO) in delayed radiation-induced skin injury, including impaired...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-11-01
|
| Series: | Applied Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3417/11/21/10290 |
| _version_ | 1797512813132906496 |
|---|---|
| author | Yi-Chi Hung Shu-Chih Hsieh Syuan-Ren Hou Jui-Yin Kung Cheng-Ming Tang Chen-Jung Chang |
| author_facet | Yi-Chi Hung Shu-Chih Hsieh Syuan-Ren Hou Jui-Yin Kung Cheng-Ming Tang Chen-Jung Chang |
| author_sort | Yi-Chi Hung |
| collection | DOAJ |
| description | Radiation-induced skin injury (RSI) is a frequent complication of radiotherapy. Severe cases affect the patient’s disease treatment and quality of life. The main purpose of this study is to examine the causative role of egg-yolk oil (EYO) in delayed radiation-induced skin injury, including impaired wound healing. Using polyvinyl pyrrolidone (PVP)-gelatin-chitosan (PGC) composite film as controlled release of egg-yolk oil (EYO) was studied in rats, which were used as a radiation-induced skin injury model. Different composition ratios were tested for their mechanical properties, moisture content, and degradation characteristics. The results showed that the PGC112 (<i>w</i>/<i>w</i>) substrate, which will hence be referred to as PGC, had the better properties. Further, the PGC was mixed with EYO (0.25% and 0.5%) to prepare the films (PGC/0.25EYO and PGC/0.5EYO, respectively). Furthermore, the mechanical and degradation properties and biocompatibility showed better results with PGC/0.5EYO. In animal studies, rats were irradiated with single-dose electron beams of 40 Gy. The animals were divided into five groups to perform wound treatment with various types of wound dressing for 30 days. Histological examination and CD68 immunochemical staining were then conducted, and observations were made. The results showed that PGC/0.5EYO has a significantly healing effects on radiodermatitis and good re-epithelialization. |
| first_indexed | 2024-03-10T06:06:58Z |
| format | Article |
| id | doaj.art-db6381efa6ee47069a9e00c74ea1fb8e |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T06:06:58Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-db6381efa6ee47069a9e00c74ea1fb8e2023-11-22T20:30:33ZengMDPI AGApplied Sciences2076-34172021-11-0111211029010.3390/app112110290In Vivo Evaluation of PVP-Gelatin-Chitosan Composite Blended with Egg-Yolk Oil for RadiodermatitisYi-Chi Hung0Shu-Chih Hsieh1Syuan-Ren Hou2Jui-Yin Kung3Cheng-Ming Tang4Chen-Jung Chang5Department of Medical Imaging and Radiological Science, Central Taiwan University of Science and Technology, Taichung 40601, TaiwanDepartment of Chemical and Materials Engineering, National Chin-Yi University of Technology, Taichung 40724, TaiwanDepartment of Medical Imaging and Radiological Science, Central Taiwan University of Science and Technology, Taichung 40601, TaiwanDepartment of Medical Imaging and Radiological Science, Central Taiwan University of Science and Technology, Taichung 40601, TaiwanGraduate Institute of Oral Science, Chung Shan Medical University, Taichung 40201, TaiwanDepartment of Medical Imaging and Radiological Science, Central Taiwan University of Science and Technology, Taichung 40601, TaiwanRadiation-induced skin injury (RSI) is a frequent complication of radiotherapy. Severe cases affect the patient’s disease treatment and quality of life. The main purpose of this study is to examine the causative role of egg-yolk oil (EYO) in delayed radiation-induced skin injury, including impaired wound healing. Using polyvinyl pyrrolidone (PVP)-gelatin-chitosan (PGC) composite film as controlled release of egg-yolk oil (EYO) was studied in rats, which were used as a radiation-induced skin injury model. Different composition ratios were tested for their mechanical properties, moisture content, and degradation characteristics. The results showed that the PGC112 (<i>w</i>/<i>w</i>) substrate, which will hence be referred to as PGC, had the better properties. Further, the PGC was mixed with EYO (0.25% and 0.5%) to prepare the films (PGC/0.25EYO and PGC/0.5EYO, respectively). Furthermore, the mechanical and degradation properties and biocompatibility showed better results with PGC/0.5EYO. In animal studies, rats were irradiated with single-dose electron beams of 40 Gy. The animals were divided into five groups to perform wound treatment with various types of wound dressing for 30 days. Histological examination and CD68 immunochemical staining were then conducted, and observations were made. The results showed that PGC/0.5EYO has a significantly healing effects on radiodermatitis and good re-epithelialization.https://www.mdpi.com/2076-3417/11/21/10290chitosangelatinpolyvinylpyrrolidoneegg-yolk oilradiodermatitiswound healing |
| spellingShingle | Yi-Chi Hung Shu-Chih Hsieh Syuan-Ren Hou Jui-Yin Kung Cheng-Ming Tang Chen-Jung Chang In Vivo Evaluation of PVP-Gelatin-Chitosan Composite Blended with Egg-Yolk Oil for Radiodermatitis Applied Sciences chitosan gelatin polyvinylpyrrolidone egg-yolk oil radiodermatitis wound healing |
| title | In Vivo Evaluation of PVP-Gelatin-Chitosan Composite Blended with Egg-Yolk Oil for Radiodermatitis |
| title_full | In Vivo Evaluation of PVP-Gelatin-Chitosan Composite Blended with Egg-Yolk Oil for Radiodermatitis |
| title_fullStr | In Vivo Evaluation of PVP-Gelatin-Chitosan Composite Blended with Egg-Yolk Oil for Radiodermatitis |
| title_full_unstemmed | In Vivo Evaluation of PVP-Gelatin-Chitosan Composite Blended with Egg-Yolk Oil for Radiodermatitis |
| title_short | In Vivo Evaluation of PVP-Gelatin-Chitosan Composite Blended with Egg-Yolk Oil for Radiodermatitis |
| title_sort | in vivo evaluation of pvp gelatin chitosan composite blended with egg yolk oil for radiodermatitis |
| topic | chitosan gelatin polyvinylpyrrolidone egg-yolk oil radiodermatitis wound healing |
| url | https://www.mdpi.com/2076-3417/11/21/10290 |
| work_keys_str_mv | AT yichihung invivoevaluationofpvpgelatinchitosancompositeblendedwitheggyolkoilforradiodermatitis AT shuchihhsieh invivoevaluationofpvpgelatinchitosancompositeblendedwitheggyolkoilforradiodermatitis AT syuanrenhou invivoevaluationofpvpgelatinchitosancompositeblendedwitheggyolkoilforradiodermatitis AT juiyinkung invivoevaluationofpvpgelatinchitosancompositeblendedwitheggyolkoilforradiodermatitis AT chengmingtang invivoevaluationofpvpgelatinchitosancompositeblendedwitheggyolkoilforradiodermatitis AT chenjungchang invivoevaluationofpvpgelatinchitosancompositeblendedwitheggyolkoilforradiodermatitis |