Metabolomic assessment of mechanisms underlying anti-renal fibrosis properties of petroleum ether extract from Amygdalus mongolica
Context The petroleum ether extract (PET) of Amygdalus mongolica (Maxim.) Ricker (Rosaceae) has an ameliorative effect on renal fibrosis (RF). Objective To evaluate the antifibrotic effects of A. mongolica seeds PET on RF by serum metabolomics, biochemical and histopathological analyses. Materials a...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-01-01
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| Series: | Pharmaceutical Biology |
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| Online Access: | http://dx.doi.org/10.1080/13880209.2021.1920619 |
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| author | Chen Gao Wan-fu Bai Hong-bing Zhou Hai-mei Hao Ying-chun Bai Quan-li Liu Hong Chang Song-li Shi |
| author_facet | Chen Gao Wan-fu Bai Hong-bing Zhou Hai-mei Hao Ying-chun Bai Quan-li Liu Hong Chang Song-li Shi |
| author_sort | Chen Gao |
| collection | DOAJ |
| description | Context The petroleum ether extract (PET) of Amygdalus mongolica (Maxim.) Ricker (Rosaceae) has an ameliorative effect on renal fibrosis (RF). Objective To evaluate the antifibrotic effects of A. mongolica seeds PET on RF by serum metabolomics, biochemical and histopathological analyses. Materials and methods Sixty male Sprague-Dawley rats were randomly divided into the sham-operated, RF model, benazepril hydrochloride-treated model (1.5 mg/kg) and PET-treated (1.75, 1.25, 0.75 g/kg) groups, and the respective drugs were administered intragastrically for 21 days. Biochemical indicators including BUN, Scr, HYP, SOD, and MDA were measured. Haematoxylin and eosin and Masson staining were used for histological examination. The serum metabolomic profiles were determined by UPLC-Q-TOF/MS and metabolism network analysis. Acute toxicity test was performed to validate biosafety. Results The PET LD50 was >23.9 g/kg in rats. PET significantly alleviated fibrosis by reducing the levels of Scr (from 34.02 to 32.02), HYP (from 403.67 to 303.17) and MDA (from 1.84 to 1.73), and increasing that of SOD (from 256.42 to 271.85). Metabolomic profiling identified 10 potential biomarkers, of which three key markers were significantly associated with RF-related pathways including phenylalanine, tyrosine and tryptophan biosynthesis, amino sugar and nucleotide sugar metabolism and tyrosine metabolism. In addition, three key biomarkers were restored to baseline levels following PET treatment, with the medium dose showing optimal effect. Conclusions These findings revealed the mechanism of A. mongolica PET antifibrotic effects for RF rats on metabolic activity and provided the experimental basis for the clinical application. |
| first_indexed | 2024-12-14T16:00:48Z |
| format | Article |
| id | doaj.art-db63d466de4b4b5ca8423ab56bb2499d |
| institution | Directory Open Access Journal |
| issn | 1388-0209 1744-5116 |
| language | English |
| last_indexed | 2024-12-14T16:00:48Z |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Pharmaceutical Biology |
| spelling | doaj.art-db63d466de4b4b5ca8423ab56bb2499d2022-12-21T22:55:12ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162021-01-0159156557410.1080/13880209.2021.19206191920619Metabolomic assessment of mechanisms underlying anti-renal fibrosis properties of petroleum ether extract from Amygdalus mongolicaChen Gao0Wan-fu Bai1Hong-bing Zhou2Hai-mei Hao3Ying-chun Bai4Quan-li Liu5Hong Chang6Song-li Shi7Department of Pharmacy, Baotou Medical College, Inner Mongolia University of Science and TechnologyDepartment of Pharmacy, Baotou Medical College, Inner Mongolia University of Science and TechnologyDepartment of Pharmacy, Baotou Medical College, Inner Mongolia University of Science and TechnologyDepartment of Pharmacy, Baotou Medical College, Inner Mongolia University of Science and TechnologyDepartment of Pharmacy, Baotou Medical College, Inner Mongolia University of Science and TechnologyDepartment of Pharmacy, Baotou Medical College, Inner Mongolia University of Science and TechnologyDepartment of Pharmacy, Baotou Medical College, Inner Mongolia University of Science and TechnologyDepartment of Pharmacy, Baotou Medical College, Inner Mongolia University of Science and TechnologyContext The petroleum ether extract (PET) of Amygdalus mongolica (Maxim.) Ricker (Rosaceae) has an ameliorative effect on renal fibrosis (RF). Objective To evaluate the antifibrotic effects of A. mongolica seeds PET on RF by serum metabolomics, biochemical and histopathological analyses. Materials and methods Sixty male Sprague-Dawley rats were randomly divided into the sham-operated, RF model, benazepril hydrochloride-treated model (1.5 mg/kg) and PET-treated (1.75, 1.25, 0.75 g/kg) groups, and the respective drugs were administered intragastrically for 21 days. Biochemical indicators including BUN, Scr, HYP, SOD, and MDA were measured. Haematoxylin and eosin and Masson staining were used for histological examination. The serum metabolomic profiles were determined by UPLC-Q-TOF/MS and metabolism network analysis. Acute toxicity test was performed to validate biosafety. Results The PET LD50 was >23.9 g/kg in rats. PET significantly alleviated fibrosis by reducing the levels of Scr (from 34.02 to 32.02), HYP (from 403.67 to 303.17) and MDA (from 1.84 to 1.73), and increasing that of SOD (from 256.42 to 271.85). Metabolomic profiling identified 10 potential biomarkers, of which three key markers were significantly associated with RF-related pathways including phenylalanine, tyrosine and tryptophan biosynthesis, amino sugar and nucleotide sugar metabolism and tyrosine metabolism. In addition, three key biomarkers were restored to baseline levels following PET treatment, with the medium dose showing optimal effect. Conclusions These findings revealed the mechanism of A. mongolica PET antifibrotic effects for RF rats on metabolic activity and provided the experimental basis for the clinical application.http://dx.doi.org/10.1080/13880209.2021.1920619traditional chinese medicinerenal fibrosisbiomarkersmetabolic pathwaymechanism research |
| spellingShingle | Chen Gao Wan-fu Bai Hong-bing Zhou Hai-mei Hao Ying-chun Bai Quan-li Liu Hong Chang Song-li Shi Metabolomic assessment of mechanisms underlying anti-renal fibrosis properties of petroleum ether extract from Amygdalus mongolica Pharmaceutical Biology traditional chinese medicine renal fibrosis biomarkers metabolic pathway mechanism research |
| title | Metabolomic assessment of mechanisms underlying anti-renal fibrosis properties of petroleum ether extract from Amygdalus mongolica |
| title_full | Metabolomic assessment of mechanisms underlying anti-renal fibrosis properties of petroleum ether extract from Amygdalus mongolica |
| title_fullStr | Metabolomic assessment of mechanisms underlying anti-renal fibrosis properties of petroleum ether extract from Amygdalus mongolica |
| title_full_unstemmed | Metabolomic assessment of mechanisms underlying anti-renal fibrosis properties of petroleum ether extract from Amygdalus mongolica |
| title_short | Metabolomic assessment of mechanisms underlying anti-renal fibrosis properties of petroleum ether extract from Amygdalus mongolica |
| title_sort | metabolomic assessment of mechanisms underlying anti renal fibrosis properties of petroleum ether extract from amygdalus mongolica |
| topic | traditional chinese medicine renal fibrosis biomarkers metabolic pathway mechanism research |
| url | http://dx.doi.org/10.1080/13880209.2021.1920619 |
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