Mechanical force induces mitochondrial fission
Eukaryotic cells are densely packed with macromolecular complexes and intertwining organelles, continually transported and reshaped. Intriguingly, organelles avoid clashing and entangling with each other in such limited space. Mitochondria form extensive networks constantly remodeled by fission and...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2017-11-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/30292 |
| _version_ | 1811180697740640256 |
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| author | Sebastian Carsten Johannes Helle Qian Feng Mathias J Aebersold Luca Hirt Raphael R Grüter Afshin Vahid Andrea Sirianni Serge Mostowy Jess G Snedeker Anđela Šarić Timon Idema Tomaso Zambelli Benoît Kornmann |
| author_facet | Sebastian Carsten Johannes Helle Qian Feng Mathias J Aebersold Luca Hirt Raphael R Grüter Afshin Vahid Andrea Sirianni Serge Mostowy Jess G Snedeker Anđela Šarić Timon Idema Tomaso Zambelli Benoît Kornmann |
| author_sort | Sebastian Carsten Johannes Helle |
| collection | DOAJ |
| description | Eukaryotic cells are densely packed with macromolecular complexes and intertwining organelles, continually transported and reshaped. Intriguingly, organelles avoid clashing and entangling with each other in such limited space. Mitochondria form extensive networks constantly remodeled by fission and fusion. Here, we show that mitochondrial fission is triggered by mechanical forces. Mechano-stimulation of mitochondria – via encounter with motile intracellular pathogens, via external pressure applied by an atomic force microscope, or via cell migration across uneven microsurfaces – results in the recruitment of the mitochondrial fission machinery, and subsequent division. We propose that MFF, owing to affinity for narrow mitochondria, acts as a membrane-bound force sensor to recruit the fission machinery to mechanically strained sites. Thus, mitochondria adapt to the environment by sensing and responding to biomechanical cues. Our findings that mechanical triggers can be coupled to biochemical responses in membrane dynamics may explain how organelles orderly cohabit in the crowded cytoplasm. |
| first_indexed | 2024-04-11T09:07:40Z |
| format | Article |
| id | doaj.art-db676d9c47a24a179b36d858b394eaa7 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:07:40Z |
| publishDate | 2017-11-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-db676d9c47a24a179b36d858b394eaa72022-12-22T04:32:36ZengeLife Sciences Publications LtdeLife2050-084X2017-11-01610.7554/eLife.30292Mechanical force induces mitochondrial fissionSebastian Carsten Johannes Helle0Qian Feng1Mathias J Aebersold2Luca Hirt3Raphael R Grüter4Afshin Vahid5https://orcid.org/0000-0001-8540-3092Andrea Sirianni6Serge Mostowy7Jess G Snedeker8Anđela Šarić9https://orcid.org/0000-0002-7854-2139Timon Idema10https://orcid.org/0000-0002-8901-5342Tomaso Zambelli11Benoît Kornmann12https://orcid.org/0000-0002-6030-8555Institute of Biochemistry, ETH Zurich, Zurich, SwitzerlandInstitute of Biochemistry, ETH Zurich, Zurich, SwitzerlandLaboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich, Zurich, SwitzerlandLaboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich, Zurich, SwitzerlandLaboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich, Zurich, SwitzerlandDepartment of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, Delft, NetherlandsSection of Microbiology, MRC Centre for Molecular Bacteriology and Infection, Imperial College London, London, United KingdomSection of Microbiology, MRC Centre for Molecular Bacteriology and Infection, Imperial College London, London, United KingdomBalgrist University Hospital, University of Zurich, Zurich, Switzerland; Institute for Biomechanics, ETH Zurich, Zurich, SwitzerlandDepartment of Physics and Astronomy, Institute for the Physics of Living Systems, University College London, London, United KingdomDepartment of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, Delft, NetherlandsLaboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich, Zurich, SwitzerlandInstitute of Biochemistry, ETH Zurich, Zurich, SwitzerlandEukaryotic cells are densely packed with macromolecular complexes and intertwining organelles, continually transported and reshaped. Intriguingly, organelles avoid clashing and entangling with each other in such limited space. Mitochondria form extensive networks constantly remodeled by fission and fusion. Here, we show that mitochondrial fission is triggered by mechanical forces. Mechano-stimulation of mitochondria – via encounter with motile intracellular pathogens, via external pressure applied by an atomic force microscope, or via cell migration across uneven microsurfaces – results in the recruitment of the mitochondrial fission machinery, and subsequent division. We propose that MFF, owing to affinity for narrow mitochondria, acts as a membrane-bound force sensor to recruit the fission machinery to mechanically strained sites. Thus, mitochondria adapt to the environment by sensing and responding to biomechanical cues. Our findings that mechanical triggers can be coupled to biochemical responses in membrane dynamics may explain how organelles orderly cohabit in the crowded cytoplasm.https://elifesciences.org/articles/30292mitochondriafissionmechanobiologydrp1mffforce |
| spellingShingle | Sebastian Carsten Johannes Helle Qian Feng Mathias J Aebersold Luca Hirt Raphael R Grüter Afshin Vahid Andrea Sirianni Serge Mostowy Jess G Snedeker Anđela Šarić Timon Idema Tomaso Zambelli Benoît Kornmann Mechanical force induces mitochondrial fission eLife mitochondria fission mechanobiology drp1 mff force |
| title | Mechanical force induces mitochondrial fission |
| title_full | Mechanical force induces mitochondrial fission |
| title_fullStr | Mechanical force induces mitochondrial fission |
| title_full_unstemmed | Mechanical force induces mitochondrial fission |
| title_short | Mechanical force induces mitochondrial fission |
| title_sort | mechanical force induces mitochondrial fission |
| topic | mitochondria fission mechanobiology drp1 mff force |
| url | https://elifesciences.org/articles/30292 |
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