Detection of Extended-Spectrum β-lactamase-producing Enterobacteriaceae in a Healthcare Center from Maracaibo city, Venezuela
The high incidence of the infectious diseases and the antimicrobial resistance arise represent a public health threat today. The extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae are an example of this phenomenon. We determined the ESBL-production in Enterobacteriaceae isolates from...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales
2017-12-01
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| Series: | Kasmera |
| Subjects: | |
| Online Access: | https://produccioncientificaluz.org/index.php/kasmera/article/view/23059 |
| _version_ | 1827852484878532608 |
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| author | Armindo Perozo Mena Marín Milagros Maribel Castellano Eliana Ling Toledo Daniela Núñez Messaria Ginestre Jessica Villasmil José Bermúdez-González Rafael Villalobos Liliana Gómez-Gamboa |
| author_facet | Armindo Perozo Mena Marín Milagros Maribel Castellano Eliana Ling Toledo Daniela Núñez Messaria Ginestre Jessica Villasmil José Bermúdez-González Rafael Villalobos Liliana Gómez-Gamboa |
| author_sort | Armindo Perozo Mena |
| collection | DOAJ |
| description | The high incidence of the infectious diseases and the antimicrobial resistance arise represent a public health threat today. The extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae are an example of this phenomenon. We determined the ESBL-production in Enterobacteriaceae isolates from a Healthcare Center in Maracaibo, during September 2014 to February 2015. The Kirby-Baüer method was perform to preliminary phenotypic detection of ESBL, according to CLSI guidelines. ESBL-production was confirmed by a double-disk synergy test according to the CLSI standards. To genotypic confirmation, the genes blaCTX-M, blaTEM and blaSHV were amplified by PCR. Fifty-five (n=55) strains were analyzed distributed in Escherichia coli (56.36 %), Klebsiella pneumoniae (21.82 %), Enterobacter cloacae (7.27 %), Proteus mirabilis and Serratia marcescens (5.45 % each one), Salmonella spp. and Morganella morganii (1.82 % each one). The major encoded ESBL was the blaTEM gene (83.63 %); followed by 23.63% of the blaCTX-M gene, and 21.81 % encoded the blaSHV gene. 27.27 % of the isolates produced two or three ESBL simultaneously. These results confirmed the high spread of this resistant mechanism among Enterobacteriaceae-producing infections in our public health institutions, therefore control measures should applied to control and reduce its incidence. |
| first_indexed | 2024-03-12T10:49:59Z |
| format | Article |
| id | doaj.art-db67ed3b35ee4ad49619f1b2ea1d5c32 |
| institution | Directory Open Access Journal |
| issn | 0075-5222 2477-9628 |
| language | English |
| last_indexed | 2024-03-12T10:49:59Z |
| publishDate | 2017-12-01 |
| publisher | Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales |
| record_format | Article |
| series | Kasmera |
| spelling | doaj.art-db67ed3b35ee4ad49619f1b2ea1d5c322023-09-02T07:06:34ZengUniversidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y TropicalesKasmera0075-52222477-96282017-12-01452889922682Detection of Extended-Spectrum β-lactamase-producing Enterobacteriaceae in a Healthcare Center from Maracaibo city, VenezuelaArmindo Perozo Mena0Marín Milagros1Maribel Castellano2Eliana Ling Toledo3Daniela Núñez4Messaria Ginestre5Jessica Villasmil6José Bermúdez-González7Rafael Villalobos8Liliana Gómez-Gamboa9Escuela de Bioanálisis. Facultad de Medicina. La Universidad del Zulia. Centro de Referencia Bacteriológica. Servicio autónomo Hospital Universitario de Maracaibo. Venezuela.Maestría de Diagnóstico Bacteriológico. División de Estudios para Graduados. Facultad de Medicina, La Universidad del Zulia. Maracaibo, VenezuelaEscuela de Bioanálisis. Facultad de Medicina. Universidad del Zulia. Maracaibo. VenezuelaEscuela de Bioanálisis. Facultad de Medicina. Universidad del Zulia. Maracaibo. VenezuelaMaestría de Diagnóstico Bacteriológico. División de Estudios para Graduados. Facultad de Medicina, La Universidad del Zulia. Maracaibo, VenezuelaEscuela de Bioanálisis. Facultad de Medicina. Universidad del Zulia. Maracaibo. VenezuelaEscuela de Bioanálisis. Facultad de Medicina. Universidad del Zulia. Maracaibo. VenezuelaBios Venezuela C.A.Cátedra de Medicina Tropical. Escuela de Medicina. Facultad de Medicina. La Universidad del Zulia. Maracaibo.VenezuelaCátedra de Microbiología. Escuela de Medicina. Facultad de Medicina. La Universidad del Zulia. Maracaibo. Venezuela.The high incidence of the infectious diseases and the antimicrobial resistance arise represent a public health threat today. The extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae are an example of this phenomenon. We determined the ESBL-production in Enterobacteriaceae isolates from a Healthcare Center in Maracaibo, during September 2014 to February 2015. The Kirby-Baüer method was perform to preliminary phenotypic detection of ESBL, according to CLSI guidelines. ESBL-production was confirmed by a double-disk synergy test according to the CLSI standards. To genotypic confirmation, the genes blaCTX-M, blaTEM and blaSHV were amplified by PCR. Fifty-five (n=55) strains were analyzed distributed in Escherichia coli (56.36 %), Klebsiella pneumoniae (21.82 %), Enterobacter cloacae (7.27 %), Proteus mirabilis and Serratia marcescens (5.45 % each one), Salmonella spp. and Morganella morganii (1.82 % each one). The major encoded ESBL was the blaTEM gene (83.63 %); followed by 23.63% of the blaCTX-M gene, and 21.81 % encoded the blaSHV gene. 27.27 % of the isolates produced two or three ESBL simultaneously. These results confirmed the high spread of this resistant mechanism among Enterobacteriaceae-producing infections in our public health institutions, therefore control measures should applied to control and reduce its incidence.https://produccioncientificaluz.org/index.php/kasmera/article/view/23059EnterobacteriasBeta-lactamasas de Espectro ExtendidoResistencia a antibióticos. |
| spellingShingle | Armindo Perozo Mena Marín Milagros Maribel Castellano Eliana Ling Toledo Daniela Núñez Messaria Ginestre Jessica Villasmil José Bermúdez-González Rafael Villalobos Liliana Gómez-Gamboa Detection of Extended-Spectrum β-lactamase-producing Enterobacteriaceae in a Healthcare Center from Maracaibo city, Venezuela Kasmera Enterobacterias Beta-lactamasas de Espectro Extendido Resistencia a antibióticos. |
| title | Detection of Extended-Spectrum β-lactamase-producing Enterobacteriaceae in a Healthcare Center from Maracaibo city, Venezuela |
| title_full | Detection of Extended-Spectrum β-lactamase-producing Enterobacteriaceae in a Healthcare Center from Maracaibo city, Venezuela |
| title_fullStr | Detection of Extended-Spectrum β-lactamase-producing Enterobacteriaceae in a Healthcare Center from Maracaibo city, Venezuela |
| title_full_unstemmed | Detection of Extended-Spectrum β-lactamase-producing Enterobacteriaceae in a Healthcare Center from Maracaibo city, Venezuela |
| title_short | Detection of Extended-Spectrum β-lactamase-producing Enterobacteriaceae in a Healthcare Center from Maracaibo city, Venezuela |
| title_sort | detection of extended spectrum β lactamase producing enterobacteriaceae in a healthcare center from maracaibo city venezuela |
| topic | Enterobacterias Beta-lactamasas de Espectro Extendido Resistencia a antibióticos. |
| url | https://produccioncientificaluz.org/index.php/kasmera/article/view/23059 |
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