Isolation and Identification of Actinomycetes Strains from Switzerland and their Biotechnological Potential
Actinomycetes strains isolated from different habitats in Switzerland were investigated for production of antibacterial and antitumoral compounds. Based on partial 16S rRNA gene sequences, the isolated strains were identified to genus level. Streptomyces as the largest genus of Actinobacteriawas iso...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | deu |
| Published: |
Swiss Chemical Society
2020-05-01
|
| Series: | CHIMIA |
| Subjects: | |
| Online Access: | https://www.ingentaconnect.com/contentone/scs/chimia/2020/00000074/00000005/art00011 |
| _version_ | 1818245342154457088 |
|---|---|
| author | Fabienne Arn David Frasson Ivana Kroslakova Fabio Rezzonico Joel F. Pothier Rainer Riedl Martin Sievers |
| author_facet | Fabienne Arn David Frasson Ivana Kroslakova Fabio Rezzonico Joel F. Pothier Rainer Riedl Martin Sievers |
| author_sort | Fabienne Arn |
| collection | DOAJ |
| description | Actinomycetes strains isolated from different habitats in Switzerland were investigated for production of antibacterial and antitumoral compounds. Based on partial 16S rRNA gene sequences, the isolated strains were identified to genus level. Streptomyces as the largest genus of Actinobacteriawas isolated the most frequently. A screening assay using the OmniLog instrument was established to facilitate the detection of active compounds from actinomycetes. Extracts prepared from the cultivated strains able to inhibit Staphylococcus aureusand Escherichia coliwere further analysed by HPLC and MALDI-TOF MS to identify the produced antibiotics. In this study, the bioactive compound echinomycin was identified from two isolated Streptomycesstrains. Natural compounds similar to TPU-0037-C, azalomycin F4a 2-ethylpentyl ester, a derivative of bafilomycin A1, milbemycin-α8 and dihydropicromycin were detected from different isolated Streptomyces strains. Milbemycin-α8 showed cytotoxic activity against HT-29 colon cancer cells. The rare actinomycete,Micromonospora sp. Stup16_C148 produced a compound that matches with the antibiotic bottromycin A2. The draft genome sequence from Actinokineospora strain B136.1 was determined using Illumina and nanopore-based technologies. The isolated strain was not able to produce antibacterial compounds under standard cultivation conditions. The antiSMASH bioinformatics analyses of the genome from strain B136.1 identified biosynthetic gene clusters with identity values between 4% to 90% to known gene clusters encoding antibiotics. The combinations of cultivation conditions, screening assays, analytical methods and genome mining are important tools to characterize strains of actinomycetes for the identification of their potential to produce natural compounds with antimicrobial activity. |
| first_indexed | 2024-12-12T14:31:23Z |
| format | Article |
| id | doaj.art-db6b8abbed524e9d81f5d82e50295f4e |
| institution | Directory Open Access Journal |
| issn | 0009-4293 2673-2424 |
| language | deu |
| last_indexed | 2024-12-12T14:31:23Z |
| publishDate | 2020-05-01 |
| publisher | Swiss Chemical Society |
| record_format | Article |
| series | CHIMIA |
| spelling | doaj.art-db6b8abbed524e9d81f5d82e50295f4e2022-12-22T00:21:31ZdeuSwiss Chemical SocietyCHIMIA0009-42932673-24242020-05-0174538239110.2533/chimia.2020.382Isolation and Identification of Actinomycetes Strains from Switzerland and their Biotechnological PotentialFabienne Arn0David Frasson1Ivana Kroslakova2Fabio Rezzonico3Joel F. Pothier4Rainer Riedl5Martin Sievers6Institute of Chemistry and Biotechnology, Organic and Medicinal Chemistry, ZHAW Zurich University of Applied Sciences, Campus Reidbach, CH-8820 Wädenswil, SwitzerlandInstitute of Chemistry and Biotechnology, Microbiology and Molecular Biology, ZHAW, Campus ReidbachInstitute of Chemistry and Biotechnology, Microbiology and Molecular Biology, ZHAW, Campus ReidbachInstitute of Natural Resource Sciences, Environmental Genomics and Systems Biology, ZHAW, Campus ReidbachInstitute of Natural Resource Sciences, Environmental Genomics and Systems Biology, ZHAW, Campus ReidbachInstitute of Chemistry and Biotechnology, Organic and Medicinal Chemistry, ZHAW Zurich University of Applied Sciences, Campus Reidbach,Institute of Chemistry and Biotechnology, Microbiology and Molecular Biology, ZHAW, Campus ReidbachActinomycetes strains isolated from different habitats in Switzerland were investigated for production of antibacterial and antitumoral compounds. Based on partial 16S rRNA gene sequences, the isolated strains were identified to genus level. Streptomyces as the largest genus of Actinobacteriawas isolated the most frequently. A screening assay using the OmniLog instrument was established to facilitate the detection of active compounds from actinomycetes. Extracts prepared from the cultivated strains able to inhibit Staphylococcus aureusand Escherichia coliwere further analysed by HPLC and MALDI-TOF MS to identify the produced antibiotics. In this study, the bioactive compound echinomycin was identified from two isolated Streptomycesstrains. Natural compounds similar to TPU-0037-C, azalomycin F4a 2-ethylpentyl ester, a derivative of bafilomycin A1, milbemycin-α8 and dihydropicromycin were detected from different isolated Streptomyces strains. Milbemycin-α8 showed cytotoxic activity against HT-29 colon cancer cells. The rare actinomycete,Micromonospora sp. Stup16_C148 produced a compound that matches with the antibiotic bottromycin A2. The draft genome sequence from Actinokineospora strain B136.1 was determined using Illumina and nanopore-based technologies. The isolated strain was not able to produce antibacterial compounds under standard cultivation conditions. The antiSMASH bioinformatics analyses of the genome from strain B136.1 identified biosynthetic gene clusters with identity values between 4% to 90% to known gene clusters encoding antibiotics. The combinations of cultivation conditions, screening assays, analytical methods and genome mining are important tools to characterize strains of actinomycetes for the identification of their potential to produce natural compounds with antimicrobial activity.https://www.ingentaconnect.com/contentone/scs/chimia/2020/00000074/00000005/art00011actinomycetesantibacterial compoundsantismashcultivationdrug discovery |
| spellingShingle | Fabienne Arn David Frasson Ivana Kroslakova Fabio Rezzonico Joel F. Pothier Rainer Riedl Martin Sievers Isolation and Identification of Actinomycetes Strains from Switzerland and their Biotechnological Potential CHIMIA actinomycetes antibacterial compounds antismash cultivation drug discovery |
| title | Isolation and Identification of Actinomycetes Strains from Switzerland and their Biotechnological Potential |
| title_full | Isolation and Identification of Actinomycetes Strains from Switzerland and their Biotechnological Potential |
| title_fullStr | Isolation and Identification of Actinomycetes Strains from Switzerland and their Biotechnological Potential |
| title_full_unstemmed | Isolation and Identification of Actinomycetes Strains from Switzerland and their Biotechnological Potential |
| title_short | Isolation and Identification of Actinomycetes Strains from Switzerland and their Biotechnological Potential |
| title_sort | isolation and identification of actinomycetes strains from switzerland and their biotechnological potential |
| topic | actinomycetes antibacterial compounds antismash cultivation drug discovery |
| url | https://www.ingentaconnect.com/contentone/scs/chimia/2020/00000074/00000005/art00011 |
| work_keys_str_mv | AT fabiennearn isolationandidentificationofactinomycetesstrainsfromswitzerlandandtheirbiotechnologicalpotential AT davidfrasson isolationandidentificationofactinomycetesstrainsfromswitzerlandandtheirbiotechnologicalpotential AT ivanakroslakova isolationandidentificationofactinomycetesstrainsfromswitzerlandandtheirbiotechnologicalpotential AT fabiorezzonico isolationandidentificationofactinomycetesstrainsfromswitzerlandandtheirbiotechnologicalpotential AT joelfpothier isolationandidentificationofactinomycetesstrainsfromswitzerlandandtheirbiotechnologicalpotential AT rainerriedl isolationandidentificationofactinomycetesstrainsfromswitzerlandandtheirbiotechnologicalpotential AT martinsievers isolationandidentificationofactinomycetesstrainsfromswitzerlandandtheirbiotechnologicalpotential |