Comparative Proteomic Analysis of Developmental Changes in P-Type Cytoplasmic Male Sterile and Maintainer Anthers in Wheat
Cytoplasmic male sterility (CMS) plays an important role in the application of heterosis in wheat (<i>Triticum aestivum</i> L.). However, the molecular mechanism underlying CMS remains unknown. This study provides a comprehensive morphological and proteomic analysis of the anthers of a P...
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2021-02-01
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| Series: | International Journal of Molecular Sciences |
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| author | Yamin Zhang Qilu Song Lili Zhang Zheng Li Chengshe Wang Gaisheng Zhang |
| author_facet | Yamin Zhang Qilu Song Lili Zhang Zheng Li Chengshe Wang Gaisheng Zhang |
| author_sort | Yamin Zhang |
| collection | DOAJ |
| description | Cytoplasmic male sterility (CMS) plays an important role in the application of heterosis in wheat (<i>Triticum aestivum</i> L.). However, the molecular mechanism underlying CMS remains unknown. This study provides a comprehensive morphological and proteomic analysis of the anthers of a P-type CMS wheat line (P) and its maintainer line, Yanshi 9 hao (Y). Cytological observations indicated that the P-type CMS line shows binucleate microspore abortion. In this line, the tapetum degraded early, leading to anther cuticle defects, which could not provide the nutrition needed for microspore development in a timely manner, thus preventing the development of the microspore to the normal binucleate stage. Proteomic analysis revealed novel proteins involved in P-type CMS. Up to 2576 differentially expressed proteins (DEPs) were quantified in all anthers, and these proteins were significantly enriched in oxidative phosphorylation, glycolysis/gluconeogenesis, citrate cycle (TCA cycle), starch and sucrose metabolism, phenylpropanoid biosynthesis, and pyruvate metabolism pathways. These proteins may comprise a network that regulates male sterility in wheat. Based on the function analysis of DEPs involved in the complex network, we concluded that the P-type CMS line may be due to cellular dysfunction caused by disturbed carbohydrate metabolism, inadequate energy supply, and disturbed protein synthesis. These results provide insights into the molecular mechanism underlying male sterility and serve as a valuable resource for researchers in plant biology, in general, and plant sexual reproduction, in particular. |
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| id | doaj.art-db6ba99d6b504c12984bd56ba7cdafc0 |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T00:47:05Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-db6ba99d6b504c12984bd56ba7cdafc02023-12-11T17:28:09ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-02-01224201210.3390/ijms22042012Comparative Proteomic Analysis of Developmental Changes in P-Type Cytoplasmic Male Sterile and Maintainer Anthers in WheatYamin Zhang0Qilu Song1Lili Zhang2Zheng Li3Chengshe Wang4Gaisheng Zhang5National Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Centre, College of Agronomy, Northwest A&F University, Yangling 712100, ChinaNational Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Centre, College of Agronomy, Northwest A&F University, Yangling 712100, ChinaNational Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Centre, College of Agronomy, Northwest A&F University, Yangling 712100, ChinaNational Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Centre, College of Agronomy, Northwest A&F University, Yangling 712100, ChinaNational Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Centre, College of Agronomy, Northwest A&F University, Yangling 712100, ChinaNational Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Centre, College of Agronomy, Northwest A&F University, Yangling 712100, ChinaCytoplasmic male sterility (CMS) plays an important role in the application of heterosis in wheat (<i>Triticum aestivum</i> L.). However, the molecular mechanism underlying CMS remains unknown. This study provides a comprehensive morphological and proteomic analysis of the anthers of a P-type CMS wheat line (P) and its maintainer line, Yanshi 9 hao (Y). Cytological observations indicated that the P-type CMS line shows binucleate microspore abortion. In this line, the tapetum degraded early, leading to anther cuticle defects, which could not provide the nutrition needed for microspore development in a timely manner, thus preventing the development of the microspore to the normal binucleate stage. Proteomic analysis revealed novel proteins involved in P-type CMS. Up to 2576 differentially expressed proteins (DEPs) were quantified in all anthers, and these proteins were significantly enriched in oxidative phosphorylation, glycolysis/gluconeogenesis, citrate cycle (TCA cycle), starch and sucrose metabolism, phenylpropanoid biosynthesis, and pyruvate metabolism pathways. These proteins may comprise a network that regulates male sterility in wheat. Based on the function analysis of DEPs involved in the complex network, we concluded that the P-type CMS line may be due to cellular dysfunction caused by disturbed carbohydrate metabolism, inadequate energy supply, and disturbed protein synthesis. These results provide insights into the molecular mechanism underlying male sterility and serve as a valuable resource for researchers in plant biology, in general, and plant sexual reproduction, in particular.https://www.mdpi.com/1422-0067/22/4/2012wheatcytoplasmic male sterilityanther proteomicsmicrospore development |
| spellingShingle | Yamin Zhang Qilu Song Lili Zhang Zheng Li Chengshe Wang Gaisheng Zhang Comparative Proteomic Analysis of Developmental Changes in P-Type Cytoplasmic Male Sterile and Maintainer Anthers in Wheat International Journal of Molecular Sciences wheat cytoplasmic male sterility anther proteomics microspore development |
| title | Comparative Proteomic Analysis of Developmental Changes in P-Type Cytoplasmic Male Sterile and Maintainer Anthers in Wheat |
| title_full | Comparative Proteomic Analysis of Developmental Changes in P-Type Cytoplasmic Male Sterile and Maintainer Anthers in Wheat |
| title_fullStr | Comparative Proteomic Analysis of Developmental Changes in P-Type Cytoplasmic Male Sterile and Maintainer Anthers in Wheat |
| title_full_unstemmed | Comparative Proteomic Analysis of Developmental Changes in P-Type Cytoplasmic Male Sterile and Maintainer Anthers in Wheat |
| title_short | Comparative Proteomic Analysis of Developmental Changes in P-Type Cytoplasmic Male Sterile and Maintainer Anthers in Wheat |
| title_sort | comparative proteomic analysis of developmental changes in p type cytoplasmic male sterile and maintainer anthers in wheat |
| topic | wheat cytoplasmic male sterility anther proteomics microspore development |
| url | https://www.mdpi.com/1422-0067/22/4/2012 |
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