Development and validation of a HPLC method for the determination of metronidazole, oxytetracycline and furazolidone in veterinary formulations
An isocratic reversed phase high performance liquid chromatographic method with DAD detection was developed for the analysis of metronidazole, oxytetracycline and furazolidone. The mobile phase consisted of pH 2.5 phosphate buffer solution, methanol and acetonitrile (70:18:12). The UV detection was...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Romanian National Association of the Veterinary Products Manufacturers
2014-06-01
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| Series: | Medicamentul Veterinar |
| Online Access: | http://www.veterinarypharmacon.com/docs/1345-2014-VD-8-1-ART.9.EN.pdf |
| _version_ | 1818567273785327616 |
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| author | Violeta Tauber (Giugiu) Viorica Chiurciu |
| author_facet | Violeta Tauber (Giugiu) Viorica Chiurciu |
| author_sort | Violeta Tauber (Giugiu) |
| collection | DOAJ |
| description | An isocratic reversed phase high performance liquid chromatographic method with DAD detection was developed for the analysis of metronidazole, oxytetracycline and furazolidone. The mobile phase consisted of pH 2.5 phosphate buffer solution, methanol and acetonitrile (70:18:12). The UV detection was carried out at 264 nm, and the flow rate was 1.100 mL/min. The separation was carried out on a Nucleosil C18 column, 5 µm 250 mmx4.6 mm, which was maintained at 25 0C. This method was validated by system suitability parameters, linearity, limits of detection and quantification, precision and accuracy |
| first_indexed | 2024-12-14T06:21:20Z |
| format | Article |
| id | doaj.art-db6bece0315e4f1882dc5bfeaf3207cb |
| institution | Directory Open Access Journal |
| issn | 1843-9527 2069-2463 |
| language | English |
| last_indexed | 2024-12-14T06:21:20Z |
| publishDate | 2014-06-01 |
| publisher | Romanian National Association of the Veterinary Products Manufacturers |
| record_format | Article |
| series | Medicamentul Veterinar |
| spelling | doaj.art-db6bece0315e4f1882dc5bfeaf3207cb2022-12-21T23:13:48ZengRomanian National Association of the Veterinary Products ManufacturersMedicamentul Veterinar1843-95272069-24632014-06-01818083Development and validation of a HPLC method for the determination of metronidazole, oxytetracycline and furazolidone in veterinary formulationsVioleta Tauber (Giugiu)0Viorica Chiurciu1Romvac Company SA, BucharestRomvac Company SA, BucharestAn isocratic reversed phase high performance liquid chromatographic method with DAD detection was developed for the analysis of metronidazole, oxytetracycline and furazolidone. The mobile phase consisted of pH 2.5 phosphate buffer solution, methanol and acetonitrile (70:18:12). The UV detection was carried out at 264 nm, and the flow rate was 1.100 mL/min. The separation was carried out on a Nucleosil C18 column, 5 µm 250 mmx4.6 mm, which was maintained at 25 0C. This method was validated by system suitability parameters, linearity, limits of detection and quantification, precision and accuracyhttp://www.veterinarypharmacon.com/docs/1345-2014-VD-8-1-ART.9.EN.pdf |
| spellingShingle | Violeta Tauber (Giugiu) Viorica Chiurciu Development and validation of a HPLC method for the determination of metronidazole, oxytetracycline and furazolidone in veterinary formulations Medicamentul Veterinar |
| title | Development and validation of a HPLC method for the determination of metronidazole, oxytetracycline and furazolidone in veterinary formulations |
| title_full | Development and validation of a HPLC method for the determination of metronidazole, oxytetracycline and furazolidone in veterinary formulations |
| title_fullStr | Development and validation of a HPLC method for the determination of metronidazole, oxytetracycline and furazolidone in veterinary formulations |
| title_full_unstemmed | Development and validation of a HPLC method for the determination of metronidazole, oxytetracycline and furazolidone in veterinary formulations |
| title_short | Development and validation of a HPLC method for the determination of metronidazole, oxytetracycline and furazolidone in veterinary formulations |
| title_sort | development and validation of a hplc method for the determination of metronidazole oxytetracycline and furazolidone in veterinary formulations |
| url | http://www.veterinarypharmacon.com/docs/1345-2014-VD-8-1-ART.9.EN.pdf |
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