Confined-microtubule assembly shapes three-dimensional cell wall structures in xylem vessels
Abstract Properly patterned deposition of cell wall polymers is prerequisite for the morphogenesis of plant cells. A cortical microtubule array guides the two-dimensional pattern of cell wall deposition. Yet, the mechanism underlying the three-dimensional patterning of cell wall deposition is poorly...
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Nature Portfolio
2023-11-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-42487-w |
| _version_ | 1797558762986274816 |
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| author | Takema Sasaki Kei Saito Daisuke Inoue Henrik Serk Yuki Sugiyama Edouard Pesquet Yuta Shimamoto Yoshihisa Oda |
| author_facet | Takema Sasaki Kei Saito Daisuke Inoue Henrik Serk Yuki Sugiyama Edouard Pesquet Yuta Shimamoto Yoshihisa Oda |
| author_sort | Takema Sasaki |
| collection | DOAJ |
| description | Abstract Properly patterned deposition of cell wall polymers is prerequisite for the morphogenesis of plant cells. A cortical microtubule array guides the two-dimensional pattern of cell wall deposition. Yet, the mechanism underlying the three-dimensional patterning of cell wall deposition is poorly understood. In metaxylem vessels, cell wall arches are formed over numerous pit membranes, forming highly organized three-dimensional cell wall structures. Here, we show that the microtubule-associated proteins, MAP70-5 and MAP70-1, regulate arch development. The map70-1 map70-5 plants formed oblique arches in an abnormal orientation in pits. Microtubules fit the aperture of developing arches in wild-type cells, whereas microtubules in map70-1 map70-5 cells extended over the boundaries of pit arches. MAP70 caused the bending and bundling of microtubules. These results suggest that MAP70 confines microtubules within the pit apertures by altering the physical properties of microtubules, thereby directing the growth of pit arches in the proper orientation. This study provides clues to understanding how plants develop three-dimensional structure of cell walls. |
| first_indexed | 2024-03-10T17:35:03Z |
| format | Article |
| id | doaj.art-db6cb444b3ce4df188de73965bde5af0 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-10T17:35:03Z |
| publishDate | 2023-11-01 |
| publisher | Nature Portfolio |
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| series | Nature Communications |
| spelling | doaj.art-db6cb444b3ce4df188de73965bde5af02023-11-20T09:53:15ZengNature PortfolioNature Communications2041-17232023-11-0114111410.1038/s41467-023-42487-wConfined-microtubule assembly shapes three-dimensional cell wall structures in xylem vesselsTakema Sasaki0Kei Saito1Daisuke Inoue2Henrik Serk3Yuki Sugiyama4Edouard Pesquet5Yuta Shimamoto6Yoshihisa Oda7Department of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-kuDepartment of Chromosome Science, National Institute of GeneticsFactuly of Design, Kyusyu UniversityUmeå Plant Science Centre (UPSC), Department of Plant Physiology, Umeå UniversityDepartment of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-kuUmeå Plant Science Centre (UPSC), Department of Plant Physiology, Umeå UniversityDepartment of Chromosome Science, National Institute of GeneticsDepartment of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-kuAbstract Properly patterned deposition of cell wall polymers is prerequisite for the morphogenesis of plant cells. A cortical microtubule array guides the two-dimensional pattern of cell wall deposition. Yet, the mechanism underlying the three-dimensional patterning of cell wall deposition is poorly understood. In metaxylem vessels, cell wall arches are formed over numerous pit membranes, forming highly organized three-dimensional cell wall structures. Here, we show that the microtubule-associated proteins, MAP70-5 and MAP70-1, regulate arch development. The map70-1 map70-5 plants formed oblique arches in an abnormal orientation in pits. Microtubules fit the aperture of developing arches in wild-type cells, whereas microtubules in map70-1 map70-5 cells extended over the boundaries of pit arches. MAP70 caused the bending and bundling of microtubules. These results suggest that MAP70 confines microtubules within the pit apertures by altering the physical properties of microtubules, thereby directing the growth of pit arches in the proper orientation. This study provides clues to understanding how plants develop three-dimensional structure of cell walls.https://doi.org/10.1038/s41467-023-42487-w |
| spellingShingle | Takema Sasaki Kei Saito Daisuke Inoue Henrik Serk Yuki Sugiyama Edouard Pesquet Yuta Shimamoto Yoshihisa Oda Confined-microtubule assembly shapes three-dimensional cell wall structures in xylem vessels Nature Communications |
| title | Confined-microtubule assembly shapes three-dimensional cell wall structures in xylem vessels |
| title_full | Confined-microtubule assembly shapes three-dimensional cell wall structures in xylem vessels |
| title_fullStr | Confined-microtubule assembly shapes three-dimensional cell wall structures in xylem vessels |
| title_full_unstemmed | Confined-microtubule assembly shapes three-dimensional cell wall structures in xylem vessels |
| title_short | Confined-microtubule assembly shapes three-dimensional cell wall structures in xylem vessels |
| title_sort | confined microtubule assembly shapes three dimensional cell wall structures in xylem vessels |
| url | https://doi.org/10.1038/s41467-023-42487-w |
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