Ovarian Stem Cells (OSCs) from the Cryopreserved Ovarian Cortex: A Potential for Neo-Oogenesis in Women with Cancer-Treatment Related Infertility: A Case Report and a Review of Literature
Cancer treatment related infertility (CTRI) affects more than one third of young women undergoing anti-cancer protocols, inducing a premature exhaustion of the ovarian reserve. In addition to ovarian suppression by GnRHa, oocyte and cortex cryopreservation has gained interest in patients with estrog...
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MDPI AG
2022-05-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/44/5/157 |
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| author | Erica Silvestris Carla Minoia Attilio Guarini Giuseppina Opinto Antonio Negri Miriam Dellino Raffaele Tinelli Gennaro Cormio Angelo Virgilio Paradiso Giuseppe De Palma |
| author_facet | Erica Silvestris Carla Minoia Attilio Guarini Giuseppina Opinto Antonio Negri Miriam Dellino Raffaele Tinelli Gennaro Cormio Angelo Virgilio Paradiso Giuseppe De Palma |
| author_sort | Erica Silvestris |
| collection | DOAJ |
| description | Cancer treatment related infertility (CTRI) affects more than one third of young women undergoing anti-cancer protocols, inducing a premature exhaustion of the ovarian reserve. In addition to ovarian suppression by GnRHa, oocyte and cortex cryopreservation has gained interest in patients with estrogen-sensitive tumors for whom the hormonal burst to prompt the multiple follicular growth could provide a further pro-life tumor pulsing. On the other hand, cortex reimplantation implies a few drawbacks due to the unknown consistency of the follicles to be reimplanted or the risk of reintroducing malignant cells. The capability of ovarian stem cells (OCSs) from fresh ovarian cortex fragments to differentiate in vitro to mature oocytes provides a tool to overcome these drawbacks. In fact, since ovarian cortex sampling and cryopreservation is practicable before gonadotoxic treatments, the recruitment of OSCs from defrosted fragments could provide a novel opportunity to verify their suitability to be expanded in vitro as oocyte like cells (OLCs). Here, we describe in very preliminary experiments the consistency of an OSC population from a single cryopreserved ovarian cortex after thawing as well as both their viability and their suitability to be further explored in their property to differentiate in OLCs, thus reinforcing interest in stemness studies in the treatment of female CTRI. |
| first_indexed | 2024-03-10T03:06:12Z |
| format | Article |
| id | doaj.art-db6ce438e7b74c23bce1a20513aa87fd |
| institution | Directory Open Access Journal |
| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-10T03:06:12Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj.art-db6ce438e7b74c23bce1a20513aa87fd2023-11-23T10:32:32ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452022-05-014452309232010.3390/cimb44050157Ovarian Stem Cells (OSCs) from the Cryopreserved Ovarian Cortex: A Potential for Neo-Oogenesis in Women with Cancer-Treatment Related Infertility: A Case Report and a Review of LiteratureErica Silvestris0Carla Minoia1Attilio Guarini2Giuseppina Opinto3Antonio Negri4Miriam Dellino5Raffaele Tinelli6Gennaro Cormio7Angelo Virgilio Paradiso8Giuseppe De Palma9Gynecologic Oncology Unit, IRCCS Istituto Tumori “Giovanni Paolo II”, 70124 Bari, ItalyHaematology Unit, IRCCS Istituto Tumori “Giovanni Paolo II”, 70124 Bari, ItalyHaematology Unit, IRCCS Istituto Tumori “Giovanni Paolo II”, 70124 Bari, ItalyHaematology Unit, IRCCS Istituto Tumori “Giovanni Paolo II”, 70124 Bari, ItalyHaematology Unit, IRCCS Istituto Tumori “Giovanni Paolo II”, 70124 Bari, ItalyDepartment of Obstetrics and Gynecology, “San Paolo” Hospital, 70123 Bari, ItalyDepartment of Obstetrics and Gynecology, “Valle d’Itria” Hospital, 74015 Martina Franca, ItalyUnit of Obstetrics and Gynecology, Department of Biomedical Sciences and Human Oncology, University of Bari “Aldo Moro”, 70124 Bari, ItalyInstitutional BioBank, Experimental Oncology and Biobank Management Unit, IRCCS Istituto Tumori “Giovanni Paolo II”, 70124 Bari, ItalyInstitutional BioBank, Experimental Oncology and Biobank Management Unit, IRCCS Istituto Tumori “Giovanni Paolo II”, 70124 Bari, ItalyCancer treatment related infertility (CTRI) affects more than one third of young women undergoing anti-cancer protocols, inducing a premature exhaustion of the ovarian reserve. In addition to ovarian suppression by GnRHa, oocyte and cortex cryopreservation has gained interest in patients with estrogen-sensitive tumors for whom the hormonal burst to prompt the multiple follicular growth could provide a further pro-life tumor pulsing. On the other hand, cortex reimplantation implies a few drawbacks due to the unknown consistency of the follicles to be reimplanted or the risk of reintroducing malignant cells. The capability of ovarian stem cells (OCSs) from fresh ovarian cortex fragments to differentiate in vitro to mature oocytes provides a tool to overcome these drawbacks. In fact, since ovarian cortex sampling and cryopreservation is practicable before gonadotoxic treatments, the recruitment of OSCs from defrosted fragments could provide a novel opportunity to verify their suitability to be expanded in vitro as oocyte like cells (OLCs). Here, we describe in very preliminary experiments the consistency of an OSC population from a single cryopreserved ovarian cortex after thawing as well as both their viability and their suitability to be further explored in their property to differentiate in OLCs, thus reinforcing interest in stemness studies in the treatment of female CTRI.https://www.mdpi.com/1467-3045/44/5/157cancer treatment related infertility (CTRI)cryopreserved ovarian cortexoocyte like cells (OLCs)ovarian stem cells (OSCs)stem cell differentiation |
| spellingShingle | Erica Silvestris Carla Minoia Attilio Guarini Giuseppina Opinto Antonio Negri Miriam Dellino Raffaele Tinelli Gennaro Cormio Angelo Virgilio Paradiso Giuseppe De Palma Ovarian Stem Cells (OSCs) from the Cryopreserved Ovarian Cortex: A Potential for Neo-Oogenesis in Women with Cancer-Treatment Related Infertility: A Case Report and a Review of Literature Current Issues in Molecular Biology cancer treatment related infertility (CTRI) cryopreserved ovarian cortex oocyte like cells (OLCs) ovarian stem cells (OSCs) stem cell differentiation |
| title | Ovarian Stem Cells (OSCs) from the Cryopreserved Ovarian Cortex: A Potential for Neo-Oogenesis in Women with Cancer-Treatment Related Infertility: A Case Report and a Review of Literature |
| title_full | Ovarian Stem Cells (OSCs) from the Cryopreserved Ovarian Cortex: A Potential for Neo-Oogenesis in Women with Cancer-Treatment Related Infertility: A Case Report and a Review of Literature |
| title_fullStr | Ovarian Stem Cells (OSCs) from the Cryopreserved Ovarian Cortex: A Potential for Neo-Oogenesis in Women with Cancer-Treatment Related Infertility: A Case Report and a Review of Literature |
| title_full_unstemmed | Ovarian Stem Cells (OSCs) from the Cryopreserved Ovarian Cortex: A Potential for Neo-Oogenesis in Women with Cancer-Treatment Related Infertility: A Case Report and a Review of Literature |
| title_short | Ovarian Stem Cells (OSCs) from the Cryopreserved Ovarian Cortex: A Potential for Neo-Oogenesis in Women with Cancer-Treatment Related Infertility: A Case Report and a Review of Literature |
| title_sort | ovarian stem cells oscs from the cryopreserved ovarian cortex a potential for neo oogenesis in women with cancer treatment related infertility a case report and a review of literature |
| topic | cancer treatment related infertility (CTRI) cryopreserved ovarian cortex oocyte like cells (OLCs) ovarian stem cells (OSCs) stem cell differentiation |
| url | https://www.mdpi.com/1467-3045/44/5/157 |
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