The effect of gelatinase production of Enterococcus faecalis on adhesion to dentin after irrigation with various endodontic irrigants
Objectives: The aim of this study was to evaluate whether the gelatinase production ability of Enterococcus faecalis provides any advantage on adhesion of this bacterium to dentin treated with various irrigants and their combinations. Materials and methods: Standardized dentin discs were randomly di...
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| Format: | Article |
| Language: | English |
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Medical Journals Sweden
2016-12-01
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| Series: | Acta Biomaterialia Odontologica Scandinavica |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/23337931.2016.1256212 |
| _version_ | 1826907976755052544 |
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| author | Mehmet Burak Guneser Ayce Unverdi Eldeniz |
| author_facet | Mehmet Burak Guneser Ayce Unverdi Eldeniz |
| author_sort | Mehmet Burak Guneser |
| collection | DOAJ |
| description | Objectives: The aim of this study was to evaluate whether the gelatinase production ability of Enterococcus faecalis provides any advantage on adhesion of this bacterium to dentin treated with various irrigants and their combinations. Materials and methods: Standardized dentin discs were randomly divided into five groups (n = 20): group 1: 2.5% sodium hypochlorite (NaOCl), group 2: 2% chlorhexidine (CHX), group 3: NaOCl + Saline + CHX, group 4: NaOCl + EDTA + NaOCl, group 5: QMix. After incubation of dentin discs with irrigants, each group was divided into two subgroups (n = 10) according to the bacterial strains used; a gelatinase-producing and a gelatinase-deficient strain of E. faecalis. After incubation of the discs with the bacterial suspensions aerobically for 48 h, XTT assay was conducted for bacterial adherence evaluation. Data were statistically analyzed by ANOVA and Tukey’s HSD tests (p = .05). Results: Gelatinase-producing E. faecalis adhered to dentin was significantly more than gelatinase-deficient E. faecalis in all test groups (p < .05). Adherence to CHX-treated dentin was lower than to the surfaces treated with other irrigants, alone or in combination (p < .05). These differences were significant except for comparisons with QMix for gelatinase-producing bacteria (p < .05). Conclusions: Gelatinase production of E. faecalis may be an important factor for bacterial adhesion. The addition of CHX to the irrigation regimen resulted in fewer adhered bacteria to dentin. QMix was not as effective as CHX in terms of bacterial adhesion prevention. |
| first_indexed | 2024-12-13T23:27:25Z |
| format | Article |
| id | doaj.art-db6e76e6c7d742b7abee7f5d460d2a50 |
| institution | Directory Open Access Journal |
| issn | 2333-7931 |
| language | English |
| last_indexed | 2025-02-17T09:16:10Z |
| publishDate | 2016-12-01 |
| publisher | Medical Journals Sweden |
| record_format | Article |
| series | Acta Biomaterialia Odontologica Scandinavica |
| spelling | doaj.art-db6e76e6c7d742b7abee7f5d460d2a502025-01-02T14:11:45ZengMedical Journals SwedenActa Biomaterialia Odontologica Scandinavica2333-79312016-12-012114414910.1080/23337931.2016.12562121256212The effect of gelatinase production of Enterococcus faecalis on adhesion to dentin after irrigation with various endodontic irrigantsMehmet Burak Guneser0Ayce Unverdi Eldeniz1Bezmialem Vakif UniversitySelcuk UniversityObjectives: The aim of this study was to evaluate whether the gelatinase production ability of Enterococcus faecalis provides any advantage on adhesion of this bacterium to dentin treated with various irrigants and their combinations. Materials and methods: Standardized dentin discs were randomly divided into five groups (n = 20): group 1: 2.5% sodium hypochlorite (NaOCl), group 2: 2% chlorhexidine (CHX), group 3: NaOCl + Saline + CHX, group 4: NaOCl + EDTA + NaOCl, group 5: QMix. After incubation of dentin discs with irrigants, each group was divided into two subgroups (n = 10) according to the bacterial strains used; a gelatinase-producing and a gelatinase-deficient strain of E. faecalis. After incubation of the discs with the bacterial suspensions aerobically for 48 h, XTT assay was conducted for bacterial adherence evaluation. Data were statistically analyzed by ANOVA and Tukey’s HSD tests (p = .05). Results: Gelatinase-producing E. faecalis adhered to dentin was significantly more than gelatinase-deficient E. faecalis in all test groups (p < .05). Adherence to CHX-treated dentin was lower than to the surfaces treated with other irrigants, alone or in combination (p < .05). These differences were significant except for comparisons with QMix for gelatinase-producing bacteria (p < .05). Conclusions: Gelatinase production of E. faecalis may be an important factor for bacterial adhesion. The addition of CHX to the irrigation regimen resulted in fewer adhered bacteria to dentin. QMix was not as effective as CHX in terms of bacterial adhesion prevention.http://dx.doi.org/10.1080/23337931.2016.1256212Dentin adhesion; Enterococcus faecalisgelatinaseirrigationQMix |
| spellingShingle | Mehmet Burak Guneser Ayce Unverdi Eldeniz The effect of gelatinase production of Enterococcus faecalis on adhesion to dentin after irrigation with various endodontic irrigants Acta Biomaterialia Odontologica Scandinavica Dentin adhesion; Enterococcus faecalis gelatinase irrigation QMix |
| title | The effect of gelatinase production of Enterococcus faecalis on adhesion to dentin after irrigation with various endodontic irrigants |
| title_full | The effect of gelatinase production of Enterococcus faecalis on adhesion to dentin after irrigation with various endodontic irrigants |
| title_fullStr | The effect of gelatinase production of Enterococcus faecalis on adhesion to dentin after irrigation with various endodontic irrigants |
| title_full_unstemmed | The effect of gelatinase production of Enterococcus faecalis on adhesion to dentin after irrigation with various endodontic irrigants |
| title_short | The effect of gelatinase production of Enterococcus faecalis on adhesion to dentin after irrigation with various endodontic irrigants |
| title_sort | effect of gelatinase production of enterococcus faecalis on adhesion to dentin after irrigation with various endodontic irrigants |
| topic | Dentin adhesion; Enterococcus faecalis gelatinase irrigation QMix |
| url | http://dx.doi.org/10.1080/23337931.2016.1256212 |
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