Metabolic engineering of fast-growing Vibrio natriegens for efficient pyruvate production
Abstract Background Pyruvate is a widely used value-added chemical which also serves as a hub of various metabolic pathways. The fastest-growing bacterium Vibrio natriegens is a promising chassis for synthetic biology applications with high substrate uptake rates. The aim of this study was to invest...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-09-01
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| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12934-023-02185-0 |
| _version_ | 1797555556349640704 |
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| author | Fengli Wu Shucai Wang Yanfeng Peng Yufeng Guo Qinhong Wang |
| author_facet | Fengli Wu Shucai Wang Yanfeng Peng Yufeng Guo Qinhong Wang |
| author_sort | Fengli Wu |
| collection | DOAJ |
| description | Abstract Background Pyruvate is a widely used value-added chemical which also serves as a hub of various metabolic pathways. The fastest-growing bacterium Vibrio natriegens is a promising chassis for synthetic biology applications with high substrate uptake rates. The aim of this study was to investigate if the high substrate uptake rates of V. natriegens enable pyruvate production at high productivities. Results Two prophage gene clusters and several essential genes for the biosynthesis of byproducts were first deleted. In order to promote pyruvate accumulation, the key gene aceE encoding pyruvate dehydrogenase complex E1 component was down-regulated to reduce the carbon flux into the tricarboxylic acid cycle. Afterwards, the expression of ppc gene encoding phosphoenolpyruvate carboxylase was fine-tuned to balance the cell growth and pyruvate synthesis. The resulting strain PYR32 was able to produce 54.22 g/L pyruvate from glucose within 16 h, with a yield of 1.17 mol/mol and an average productivity of 3.39 g/L/h. In addition, this strain was also able to efficiently convert sucrose or gluconate into pyruvate at high titers. Conclusion A novel strain of V. natriegens was engineered which was capable to provide higher productivity in pyruvate synthesis. This study lays the foundation for the biosynthesis of pyruvate and its derivatives in fast-growing V. natriegens. |
| first_indexed | 2024-03-10T16:49:12Z |
| format | Article |
| id | doaj.art-db71cb79cf0e456cb158e9f38c683a0f |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-03-10T16:49:12Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-db71cb79cf0e456cb158e9f38c683a0f2023-11-20T11:22:44ZengBMCMicrobial Cell Factories1475-28592023-09-0122111010.1186/s12934-023-02185-0Metabolic engineering of fast-growing Vibrio natriegens for efficient pyruvate productionFengli Wu0Shucai Wang1Yanfeng Peng2Yufeng Guo3Qinhong Wang4Tianjin Institute of Industrial Biotechnology, Chinese Academy of SciencesTianjin Institute of Industrial Biotechnology, Chinese Academy of SciencesTianjin Institute of Industrial Biotechnology, Chinese Academy of SciencesTianjin Institute of Industrial Biotechnology, Chinese Academy of SciencesTianjin Institute of Industrial Biotechnology, Chinese Academy of SciencesAbstract Background Pyruvate is a widely used value-added chemical which also serves as a hub of various metabolic pathways. The fastest-growing bacterium Vibrio natriegens is a promising chassis for synthetic biology applications with high substrate uptake rates. The aim of this study was to investigate if the high substrate uptake rates of V. natriegens enable pyruvate production at high productivities. Results Two prophage gene clusters and several essential genes for the biosynthesis of byproducts were first deleted. In order to promote pyruvate accumulation, the key gene aceE encoding pyruvate dehydrogenase complex E1 component was down-regulated to reduce the carbon flux into the tricarboxylic acid cycle. Afterwards, the expression of ppc gene encoding phosphoenolpyruvate carboxylase was fine-tuned to balance the cell growth and pyruvate synthesis. The resulting strain PYR32 was able to produce 54.22 g/L pyruvate from glucose within 16 h, with a yield of 1.17 mol/mol and an average productivity of 3.39 g/L/h. In addition, this strain was also able to efficiently convert sucrose or gluconate into pyruvate at high titers. Conclusion A novel strain of V. natriegens was engineered which was capable to provide higher productivity in pyruvate synthesis. This study lays the foundation for the biosynthesis of pyruvate and its derivatives in fast-growing V. natriegens.https://doi.org/10.1186/s12934-023-02185-0Vibrio natriegensPyruvatePathway engineeringFine-tuning gene expressionFermentation |
| spellingShingle | Fengli Wu Shucai Wang Yanfeng Peng Yufeng Guo Qinhong Wang Metabolic engineering of fast-growing Vibrio natriegens for efficient pyruvate production Microbial Cell Factories Vibrio natriegens Pyruvate Pathway engineering Fine-tuning gene expression Fermentation |
| title | Metabolic engineering of fast-growing Vibrio natriegens for efficient pyruvate production |
| title_full | Metabolic engineering of fast-growing Vibrio natriegens for efficient pyruvate production |
| title_fullStr | Metabolic engineering of fast-growing Vibrio natriegens for efficient pyruvate production |
| title_full_unstemmed | Metabolic engineering of fast-growing Vibrio natriegens for efficient pyruvate production |
| title_short | Metabolic engineering of fast-growing Vibrio natriegens for efficient pyruvate production |
| title_sort | metabolic engineering of fast growing vibrio natriegens for efficient pyruvate production |
| topic | Vibrio natriegens Pyruvate Pathway engineering Fine-tuning gene expression Fermentation |
| url | https://doi.org/10.1186/s12934-023-02185-0 |
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