Spermatogenesis and nucleolar activity in Triatoma klugi (triatomine, Heteroptera)
Triatoma klugi is a Chagas disease vector in the Rio Grande do Sul State. Triatominae chromosomes are holocentric and sex chromosomes segregation is post-reductional. In this paper we describe the karyotype of male T. klugi and a meiotic analysis including the nucleolar behavior during spermatogenes...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2008-01-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300008 |
| _version_ | 1819210410662821888 |
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| author | Laiana Cristina da Costa Maria Tercília Vilela de Azeredo-Oliveira Ester Tartarotti |
| author_facet | Laiana Cristina da Costa Maria Tercília Vilela de Azeredo-Oliveira Ester Tartarotti |
| author_sort | Laiana Cristina da Costa |
| collection | DOAJ |
| description | Triatoma klugi is a Chagas disease vector in the Rio Grande do Sul State. Triatominae chromosomes are holocentric and sex chromosomes segregation is post-reductional. In this paper we describe the karyotype of male T. klugi and a meiotic analysis including the nucleolar behavior during spermatogenesis. Testis cells were analyzed after lacto-acetic orcein and silver nitrate staining. Two autosomes and the heterochromosomes presented nucleolar activity (Ag-NORs) during diplotene-diakinesis. The analysis of metaphase I and II revealed a karyotype with 2n = 20+XY. In metaphase I a prominent nucleolar mass was observed in the cell periphery and small silver grains were detected in metaphase II. During anaphase, the chromosomes segregated in parallel and a typical holocentric late migration behavior was observed. The restoration of the nucleolus was an important feature in this phase. During telophase nucleolar masses persisted and in early spermiogenesis the spermatids presented a small peripheral mass until elongation. The present study is a contribution to the study of chromatin behavior and nucleolar persistence in meiosis. |
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| format | Article |
| id | doaj.art-db72e0db102a4277b0e9aab5060cb8e2 |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-23T06:10:44Z |
| publishDate | 2008-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-db72e0db102a4277b0e9aab5060cb8e22022-12-21T17:57:27ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852008-01-0131243844410.1590/S1415-47572008000300008Spermatogenesis and nucleolar activity in Triatoma klugi (triatomine, Heteroptera)Laiana Cristina da CostaMaria Tercília Vilela de Azeredo-OliveiraEster TartarottiTriatoma klugi is a Chagas disease vector in the Rio Grande do Sul State. Triatominae chromosomes are holocentric and sex chromosomes segregation is post-reductional. In this paper we describe the karyotype of male T. klugi and a meiotic analysis including the nucleolar behavior during spermatogenesis. Testis cells were analyzed after lacto-acetic orcein and silver nitrate staining. Two autosomes and the heterochromosomes presented nucleolar activity (Ag-NORs) during diplotene-diakinesis. The analysis of metaphase I and II revealed a karyotype with 2n = 20+XY. In metaphase I a prominent nucleolar mass was observed in the cell periphery and small silver grains were detected in metaphase II. During anaphase, the chromosomes segregated in parallel and a typical holocentric late migration behavior was observed. The restoration of the nucleolus was an important feature in this phase. During telophase nucleolar masses persisted and in early spermiogenesis the spermatids presented a small peripheral mass until elongation. The present study is a contribution to the study of chromatin behavior and nucleolar persistence in meiosis.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300008Triatoma klugiTriatominecytogeneticsnucleoluskaryotypemeiosis |
| spellingShingle | Laiana Cristina da Costa Maria Tercília Vilela de Azeredo-Oliveira Ester Tartarotti Spermatogenesis and nucleolar activity in Triatoma klugi (triatomine, Heteroptera) Genetics and Molecular Biology Triatoma klugi Triatomine cytogenetics nucleolus karyotype meiosis |
| title | Spermatogenesis and nucleolar activity in Triatoma klugi (triatomine, Heteroptera) |
| title_full | Spermatogenesis and nucleolar activity in Triatoma klugi (triatomine, Heteroptera) |
| title_fullStr | Spermatogenesis and nucleolar activity in Triatoma klugi (triatomine, Heteroptera) |
| title_full_unstemmed | Spermatogenesis and nucleolar activity in Triatoma klugi (triatomine, Heteroptera) |
| title_short | Spermatogenesis and nucleolar activity in Triatoma klugi (triatomine, Heteroptera) |
| title_sort | spermatogenesis and nucleolar activity in triatoma klugi triatomine heteroptera |
| topic | Triatoma klugi Triatomine cytogenetics nucleolus karyotype meiosis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300008 |
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