Mixed infections with <it>Chlamydia </it>and porcine epidemic diarrhea virus - a new <it>in vitro </it>model of chlamydial persistence
<p>Abstract</p> <p>Background</p> <p>Chlamydiae induce persistent infections, which have been associated with a wide range of chronic diseases in humans and animals. Mixed infections with <it>Chlamydia </it>and porcine epidemic diarrhea virus (PEDV) may resu...
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| Format: | Article |
| Language: | English |
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BMC
2010-07-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/10/201 |
| _version_ | 1811283401020276736 |
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| author | Kaiser Carmen Schifferli Andrea Ziegler Urs Dumrese Claudia Borel Nicole Pospischil Andreas |
| author_facet | Kaiser Carmen Schifferli Andrea Ziegler Urs Dumrese Claudia Borel Nicole Pospischil Andreas |
| author_sort | Kaiser Carmen |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Chlamydiae induce persistent infections, which have been associated with a wide range of chronic diseases in humans and animals. Mixed infections with <it>Chlamydia </it>and porcine epidemic diarrhea virus (PEDV) may result in generation of persistent chlamydial infections. To test this hypothesis, an <it>in vitro </it>model of dual infection with cell culture-adapted PEDV and <it>Chlamydia abortus </it>or <it>Chlamydia pecorum </it>in Vero cells was established.</p> <p>Results</p> <p>Infected cultures were investigated by immunofluorescence (IF), transmission electron microscopy (TEM) and re-infection experiments. By IF, <it>Chlamydia</it>-infected cells showed normal inclusions after 39 hpi. Dual infections with <it>Chlamydia abortus </it>revealed a heterogenous mix of inclusion types including small inclusions consisting of aberrant bodies (ABs), medium-sized inclusions consisting of ABs and reticulate bodies and normal inclusions. Only aberrant inclusions were observable in dual infection experiments with <it>Chlamydia pecorum </it>and PEDV. TEM examinations of mixed infections with <it>Chlamydia abortus </it>and <it>Chlamydia pecorum </it>revealed aberrant chlamydial inclusions containing reticulate-like, pleomorphic ABs, which were up to 2 μm in diameter. No re-differentiation into elementary bodies (EBs) was detected. In re-infection experiments, co-infected cells produced fewer EBs than monoinfected cells.</p> <p>Conclusions</p> <p>In the present study we confirm that PEDV co-infection alters the developmental cycle of member species of the family <it>Chlamydiaceae</it>, in a similar manner to other well-described persistence induction methods. Interestingly, this effect appears to be partially species-specific as <it>Chlamydia pecorum </it>appears more sensitive to PEDV co-infection than <it>Chlamydia abortus</it>, as evidenced by TEM and IF observations of a homogenous population of aberrant inclusions in PEDV - <it>Chlamydia pecorum </it>co-infections.</p> |
| first_indexed | 2024-04-13T02:12:05Z |
| format | Article |
| id | doaj.art-db7617a7f15d4cb589003084b6bf0f2f |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-04-13T02:12:05Z |
| publishDate | 2010-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-db7617a7f15d4cb589003084b6bf0f2f2022-12-22T03:07:17ZengBMCBMC Microbiology1471-21802010-07-0110120110.1186/1471-2180-10-201Mixed infections with <it>Chlamydia </it>and porcine epidemic diarrhea virus - a new <it>in vitro </it>model of chlamydial persistenceKaiser CarmenSchifferli AndreaZiegler UrsDumrese ClaudiaBorel NicolePospischil Andreas<p>Abstract</p> <p>Background</p> <p>Chlamydiae induce persistent infections, which have been associated with a wide range of chronic diseases in humans and animals. Mixed infections with <it>Chlamydia </it>and porcine epidemic diarrhea virus (PEDV) may result in generation of persistent chlamydial infections. To test this hypothesis, an <it>in vitro </it>model of dual infection with cell culture-adapted PEDV and <it>Chlamydia abortus </it>or <it>Chlamydia pecorum </it>in Vero cells was established.</p> <p>Results</p> <p>Infected cultures were investigated by immunofluorescence (IF), transmission electron microscopy (TEM) and re-infection experiments. By IF, <it>Chlamydia</it>-infected cells showed normal inclusions after 39 hpi. Dual infections with <it>Chlamydia abortus </it>revealed a heterogenous mix of inclusion types including small inclusions consisting of aberrant bodies (ABs), medium-sized inclusions consisting of ABs and reticulate bodies and normal inclusions. Only aberrant inclusions were observable in dual infection experiments with <it>Chlamydia pecorum </it>and PEDV. TEM examinations of mixed infections with <it>Chlamydia abortus </it>and <it>Chlamydia pecorum </it>revealed aberrant chlamydial inclusions containing reticulate-like, pleomorphic ABs, which were up to 2 μm in diameter. No re-differentiation into elementary bodies (EBs) was detected. In re-infection experiments, co-infected cells produced fewer EBs than monoinfected cells.</p> <p>Conclusions</p> <p>In the present study we confirm that PEDV co-infection alters the developmental cycle of member species of the family <it>Chlamydiaceae</it>, in a similar manner to other well-described persistence induction methods. Interestingly, this effect appears to be partially species-specific as <it>Chlamydia pecorum </it>appears more sensitive to PEDV co-infection than <it>Chlamydia abortus</it>, as evidenced by TEM and IF observations of a homogenous population of aberrant inclusions in PEDV - <it>Chlamydia pecorum </it>co-infections.</p>http://www.biomedcentral.com/1471-2180/10/201 |
| spellingShingle | Kaiser Carmen Schifferli Andrea Ziegler Urs Dumrese Claudia Borel Nicole Pospischil Andreas Mixed infections with <it>Chlamydia </it>and porcine epidemic diarrhea virus - a new <it>in vitro </it>model of chlamydial persistence BMC Microbiology |
| title | Mixed infections with <it>Chlamydia </it>and porcine epidemic diarrhea virus - a new <it>in vitro </it>model of chlamydial persistence |
| title_full | Mixed infections with <it>Chlamydia </it>and porcine epidemic diarrhea virus - a new <it>in vitro </it>model of chlamydial persistence |
| title_fullStr | Mixed infections with <it>Chlamydia </it>and porcine epidemic diarrhea virus - a new <it>in vitro </it>model of chlamydial persistence |
| title_full_unstemmed | Mixed infections with <it>Chlamydia </it>and porcine epidemic diarrhea virus - a new <it>in vitro </it>model of chlamydial persistence |
| title_short | Mixed infections with <it>Chlamydia </it>and porcine epidemic diarrhea virus - a new <it>in vitro </it>model of chlamydial persistence |
| title_sort | mixed infections with it chlamydia it and porcine epidemic diarrhea virus a new it in vitro it model of chlamydial persistence |
| url | http://www.biomedcentral.com/1471-2180/10/201 |
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