Single-Cell Sequencing on Marine Life: Application and Future Development
Single-cell RNA-sequencing (scRNA-seq) is the genomic approach that directly examines gene expression information from individual cells and provides a higher resolution of cellular differences than bulk RNA-seq. In the past decade, with the rapid development of microfluid, barcoding, and next-genera...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Marine Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2022.906267/full |
| _version_ | 1811241611484463104 |
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| author | Jing Li Jing Li Hao Wang Chaolun Li Chaolun Li Chaolun Li |
| author_facet | Jing Li Jing Li Hao Wang Chaolun Li Chaolun Li Chaolun Li |
| author_sort | Jing Li |
| collection | DOAJ |
| description | Single-cell RNA-sequencing (scRNA-seq) is the genomic approach that directly examines gene expression information from individual cells and provides a higher resolution of cellular differences than bulk RNA-seq. In the past decade, with the rapid development of microfluid, barcoding, and next-generation sequencing (NGS) technologies, scRNA-seq has revolutionized transcriptomic studies and gained great success and broad prospects. However, compared with the wide use in the few model animals, the application of scRNA-seq in marine organisms is limited due to the high cost of early scRNA-seq and the immature of single-cell methods for marine organisms. Recently, with the increase of genomic data, maturation of scRNA-seq platform and downstream bioinformatics algorithms, the scRNA-seq has been successfully applied in several marine model animals with great success, which demonstrated that the scRNA-seq could be the ideal and powerful tool to extend our understanding of marine organisms’ evolutionary and physiological processes and their adaptation to ecological niches. Here, we review the state-of-the-art improvements of single-cell sequencing techniques and new studies that apply single-cell methods to marine organisms. We also summarize the opportunities and challenges scientists may face in further single-cell research and propose several appealing prospects that may benefit from the combination of single-cell techniques and marine organisms. |
| first_indexed | 2024-04-12T13:39:39Z |
| format | Article |
| id | doaj.art-db7c153dff3649af95854fe31ab0ea22 |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-04-12T13:39:39Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-db7c153dff3649af95854fe31ab0ea222022-12-22T03:30:54ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452022-06-01910.3389/fmars.2022.906267906267Single-Cell Sequencing on Marine Life: Application and Future DevelopmentJing Li0Jing Li1Hao Wang2Chaolun Li3Chaolun Li4Chaolun Li5Center of Deep-Sea Research, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaCenter of Deep-Sea Research, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaCenter of Deep-Sea Research, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaLaboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaSingle-cell RNA-sequencing (scRNA-seq) is the genomic approach that directly examines gene expression information from individual cells and provides a higher resolution of cellular differences than bulk RNA-seq. In the past decade, with the rapid development of microfluid, barcoding, and next-generation sequencing (NGS) technologies, scRNA-seq has revolutionized transcriptomic studies and gained great success and broad prospects. However, compared with the wide use in the few model animals, the application of scRNA-seq in marine organisms is limited due to the high cost of early scRNA-seq and the immature of single-cell methods for marine organisms. Recently, with the increase of genomic data, maturation of scRNA-seq platform and downstream bioinformatics algorithms, the scRNA-seq has been successfully applied in several marine model animals with great success, which demonstrated that the scRNA-seq could be the ideal and powerful tool to extend our understanding of marine organisms’ evolutionary and physiological processes and their adaptation to ecological niches. Here, we review the state-of-the-art improvements of single-cell sequencing techniques and new studies that apply single-cell methods to marine organisms. We also summarize the opportunities and challenges scientists may face in further single-cell research and propose several appealing prospects that may benefit from the combination of single-cell techniques and marine organisms.https://www.frontiersin.org/articles/10.3389/fmars.2022.906267/fullscRNA-seqmarine organismsnRNA-seqfuture directionscell isolation method |
| spellingShingle | Jing Li Jing Li Hao Wang Chaolun Li Chaolun Li Chaolun Li Single-Cell Sequencing on Marine Life: Application and Future Development Frontiers in Marine Science scRNA-seq marine organism snRNA-seq future directions cell isolation method |
| title | Single-Cell Sequencing on Marine Life: Application and Future Development |
| title_full | Single-Cell Sequencing on Marine Life: Application and Future Development |
| title_fullStr | Single-Cell Sequencing on Marine Life: Application and Future Development |
| title_full_unstemmed | Single-Cell Sequencing on Marine Life: Application and Future Development |
| title_short | Single-Cell Sequencing on Marine Life: Application and Future Development |
| title_sort | single cell sequencing on marine life application and future development |
| topic | scRNA-seq marine organism snRNA-seq future directions cell isolation method |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2022.906267/full |
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