Comparative Study of <i>Plantago media</i> Extracts in the Treatment of <i>Acanthamoeba</i> sp. Trophozoites
(1) Background: The aim of the study was to compare the potency of <i>Plantago media</i> L. (Plantaginaceae) extracts on <i>Acanthamoeba</i> sp. trophozoites, which are opportunistic protozoan parasites leading to several dangerous diseases; (2) Methods: The chromatographical...
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MDPI AG
2023-06-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/13/12/7075 |
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| author | Anna Budzianowska Monika Derda Jaromir Budzianowski Agnieszka Szopa Małgorzata Kikowska |
| author_facet | Anna Budzianowska Monika Derda Jaromir Budzianowski Agnieszka Szopa Małgorzata Kikowska |
| author_sort | Anna Budzianowska |
| collection | DOAJ |
| description | (1) Background: The aim of the study was to compare the potency of <i>Plantago media</i> L. (Plantaginaceae) extracts on <i>Acanthamoeba</i> sp. trophozoites, which are opportunistic protozoan parasites leading to several dangerous diseases; (2) Methods: The chromatographically (TLC, HPLC-DAD) characterized water fractions of the extracts from biomass from in vitro cultures (shoots and roots), leaves, and inflorescences from field cultivation were used for the study of the acanthamoebic activity in a Thoma haemocytometer chamber; (3) Results: The anti-amoebic effect at the lowest concentration (1.0 mg/mL) was demonstrated only by the extract of the leaves from the cultivation (50.50% inhibition). The remaining samples inhibited the growth of parasites from a concentration of 5.0 mg/mL in the range of 41.36% inflorescences to 63.89% shoots in vitro. Quantitative determinations of phenolic compounds in the tested extracts indicate a tendency to increase the potency of the anti-amoebic effect with the content of a phenylethanoid glycoside—acteoside. The maximum content of this compound was determined in leaves from field cultivation (6.64%) and the minimum in inflorescences (0.65%). This is confirmed by the range of the lowest IC<sub>50</sub> values (the strongest biological activity) for the tested samples, 0.95–1.80 mg/mL for leaves from cultivation, and the high values, 9.70–5.30 mg/mL for inflorescences and in-vitro-derived roots. The strength of the biological activity of the extracts correlated with the content of acteoside, which constituted 84–93% of the sum of phenolic compounds determined; (4) Conclusions: The performed investigations proved the anti-acanthamoebic efficacy of <i>Plantago media</i> organs, including those obtainable by biotechnological methods, and indicated phenylethanoid glycosides, their main phenolic constituents, to be responsible for the activity. To our knowledge, this is the first report on the amoebicidal activity of <i>Plantago media</i> extracts from biomass produced by biotechnological methods and organs of an intact plant. |
| first_indexed | 2024-03-11T02:49:09Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-11T02:49:09Z |
| publishDate | 2023-06-01 |
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| spelling | doaj.art-db7dbc54a3c2430196a61acb119c19f62023-11-18T09:08:39ZengMDPI AGApplied Sciences2076-34172023-06-011312707510.3390/app13127075Comparative Study of <i>Plantago media</i> Extracts in the Treatment of <i>Acanthamoeba</i> sp. TrophozoitesAnna Budzianowska0Monika Derda1Jaromir Budzianowski2Agnieszka Szopa3Małgorzata Kikowska4Laboratory of Pharmaceutical Biology and Biotechnology, Department and Division of Practical Cosmetology and Skin Diseases Prophylaxis, Poznan University of Medical Sciences, 3 Rokietnicka St., 60-806 Poznań, PolandChair and Department of Biology and Medical Parasitology, Poznan University of Medical Sciences, 4 Święcickiego St., 61-781 Poznań, PolandLaboratory of Pharmaceutical Biology and Biotechnology, Department and Division of Practical Cosmetology and Skin Diseases Prophylaxis, Poznan University of Medical Sciences, 3 Rokietnicka St., 60-806 Poznań, PolandDepartment of Pharmaceutical Botany, Collegium Medicum, Jagiellonian University, 9 Medyczna St., 30-688 Kraków, PolandLaboratory of Pharmaceutical Biology and Biotechnology, Department and Division of Practical Cosmetology and Skin Diseases Prophylaxis, Poznan University of Medical Sciences, 3 Rokietnicka St., 60-806 Poznań, Poland(1) Background: The aim of the study was to compare the potency of <i>Plantago media</i> L. (Plantaginaceae) extracts on <i>Acanthamoeba</i> sp. trophozoites, which are opportunistic protozoan parasites leading to several dangerous diseases; (2) Methods: The chromatographically (TLC, HPLC-DAD) characterized water fractions of the extracts from biomass from in vitro cultures (shoots and roots), leaves, and inflorescences from field cultivation were used for the study of the acanthamoebic activity in a Thoma haemocytometer chamber; (3) Results: The anti-amoebic effect at the lowest concentration (1.0 mg/mL) was demonstrated only by the extract of the leaves from the cultivation (50.50% inhibition). The remaining samples inhibited the growth of parasites from a concentration of 5.0 mg/mL in the range of 41.36% inflorescences to 63.89% shoots in vitro. Quantitative determinations of phenolic compounds in the tested extracts indicate a tendency to increase the potency of the anti-amoebic effect with the content of a phenylethanoid glycoside—acteoside. The maximum content of this compound was determined in leaves from field cultivation (6.64%) and the minimum in inflorescences (0.65%). This is confirmed by the range of the lowest IC<sub>50</sub> values (the strongest biological activity) for the tested samples, 0.95–1.80 mg/mL for leaves from cultivation, and the high values, 9.70–5.30 mg/mL for inflorescences and in-vitro-derived roots. The strength of the biological activity of the extracts correlated with the content of acteoside, which constituted 84–93% of the sum of phenolic compounds determined; (4) Conclusions: The performed investigations proved the anti-acanthamoebic efficacy of <i>Plantago media</i> organs, including those obtainable by biotechnological methods, and indicated phenylethanoid glycosides, their main phenolic constituents, to be responsible for the activity. To our knowledge, this is the first report on the amoebicidal activity of <i>Plantago media</i> extracts from biomass produced by biotechnological methods and organs of an intact plant.https://www.mdpi.com/2076-3417/13/12/7075<i>Plantago media</i>in vitro culturesplant phenolicsphenylethanoid glycosidesacteoside (verbascoside)acanthamoebic activity |
| spellingShingle | Anna Budzianowska Monika Derda Jaromir Budzianowski Agnieszka Szopa Małgorzata Kikowska Comparative Study of <i>Plantago media</i> Extracts in the Treatment of <i>Acanthamoeba</i> sp. Trophozoites Applied Sciences <i>Plantago media</i> in vitro cultures plant phenolics phenylethanoid glycosides acteoside (verbascoside) acanthamoebic activity |
| title | Comparative Study of <i>Plantago media</i> Extracts in the Treatment of <i>Acanthamoeba</i> sp. Trophozoites |
| title_full | Comparative Study of <i>Plantago media</i> Extracts in the Treatment of <i>Acanthamoeba</i> sp. Trophozoites |
| title_fullStr | Comparative Study of <i>Plantago media</i> Extracts in the Treatment of <i>Acanthamoeba</i> sp. Trophozoites |
| title_full_unstemmed | Comparative Study of <i>Plantago media</i> Extracts in the Treatment of <i>Acanthamoeba</i> sp. Trophozoites |
| title_short | Comparative Study of <i>Plantago media</i> Extracts in the Treatment of <i>Acanthamoeba</i> sp. Trophozoites |
| title_sort | comparative study of i plantago media i extracts in the treatment of i acanthamoeba i sp trophozoites |
| topic | <i>Plantago media</i> in vitro cultures plant phenolics phenylethanoid glycosides acteoside (verbascoside) acanthamoebic activity |
| url | https://www.mdpi.com/2076-3417/13/12/7075 |
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