Substance-P Restores Cellular Activity of ADSC Impaired by Oxidative Stress
Oxidative stress induces cellular damage, which accelerates aging and promotes the development of serious illnesses. Adipose-derived stem cells (ADSCs) are novel cellular therapeutic tools and have been applied for tissue regeneration. However, ADSCs from aged and diseased individuals may be affecte...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-10-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/9/10/978 |
| _version_ | 1797551246358347776 |
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| author | Jeong Seop Park Jiyuan Piao Gabee Park Hyun Sook Hong |
| author_facet | Jeong Seop Park Jiyuan Piao Gabee Park Hyun Sook Hong |
| author_sort | Jeong Seop Park |
| collection | DOAJ |
| description | Oxidative stress induces cellular damage, which accelerates aging and promotes the development of serious illnesses. Adipose-derived stem cells (ADSCs) are novel cellular therapeutic tools and have been applied for tissue regeneration. However, ADSCs from aged and diseased individuals may be affected in vivo by the accumulation of free radicals, which can impair their therapeutic efficacy. Substance-P (SP) is a neuropeptide that is known to rescue stem cells from senescence and inflammatory attack, and this study explored the restorative effect of SP on ADSCs under oxidative stress. ADSCs were transiently exposed to H<sub>2</sub>O<sub>2</sub>, and then treated with SP. H<sub>2</sub>O<sub>2</sub> treatment decreased ADSC cell viability, proliferation, and cytokine production and this activity was not recovered even after the removal of H<sub>2</sub>O<sub>2</sub>. However, the addition of SP increased cell viability and restored paracrine potential, leading to the accelerated repopulation of ADSCs injured by H<sub>2</sub>O<sub>2</sub>. Furthermore, SP was capable of activating Akt/GSK-3β signaling, which was found to be downregulated following H<sub>2</sub>O<sub>2</sub> treatment. This might contribute to the restorative effect of SP on injured ADSCs. Collectively, SP can protect ADSCs from oxidant-induced cell damage, possibly by activating Akt/GSK-3β signaling in ADSCs. This study supports the possibility that SP can recover cell activity from oxidative stress-induced dysfunction. |
| first_indexed | 2024-03-10T15:41:51Z |
| format | Article |
| id | doaj.art-db7dd84fe1864c888ce26688edd47f43 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T15:41:51Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-db7dd84fe1864c888ce26688edd47f432023-11-20T16:47:50ZengMDPI AGAntioxidants2076-39212020-10-0191097810.3390/antiox9100978Substance-P Restores Cellular Activity of ADSC Impaired by Oxidative StressJeong Seop Park0Jiyuan Piao1Gabee Park2Hyun Sook Hong3Department of Biomedical Science and Technology, Graduate School, Kyung Hee University, Seoul 02447, KoreaDepartment of Genetic Engineering, College of Life Science and Graduate School of Biotechnology, Kyung Hee University, Yong In 17104, KoreaDepartment of Genetic Engineering, College of Life Science and Graduate School of Biotechnology, Kyung Hee University, Yong In 17104, KoreaDepartment of Biomedical Science and Technology, Graduate School, Kyung Hee University, Seoul 02447, KoreaOxidative stress induces cellular damage, which accelerates aging and promotes the development of serious illnesses. Adipose-derived stem cells (ADSCs) are novel cellular therapeutic tools and have been applied for tissue regeneration. However, ADSCs from aged and diseased individuals may be affected in vivo by the accumulation of free radicals, which can impair their therapeutic efficacy. Substance-P (SP) is a neuropeptide that is known to rescue stem cells from senescence and inflammatory attack, and this study explored the restorative effect of SP on ADSCs under oxidative stress. ADSCs were transiently exposed to H<sub>2</sub>O<sub>2</sub>, and then treated with SP. H<sub>2</sub>O<sub>2</sub> treatment decreased ADSC cell viability, proliferation, and cytokine production and this activity was not recovered even after the removal of H<sub>2</sub>O<sub>2</sub>. However, the addition of SP increased cell viability and restored paracrine potential, leading to the accelerated repopulation of ADSCs injured by H<sub>2</sub>O<sub>2</sub>. Furthermore, SP was capable of activating Akt/GSK-3β signaling, which was found to be downregulated following H<sub>2</sub>O<sub>2</sub> treatment. This might contribute to the restorative effect of SP on injured ADSCs. Collectively, SP can protect ADSCs from oxidant-induced cell damage, possibly by activating Akt/GSK-3β signaling in ADSCs. This study supports the possibility that SP can recover cell activity from oxidative stress-induced dysfunction.https://www.mdpi.com/2076-3921/9/10/978Substance-Padipose-derived stem cellsoxidative stressparacrine factors |
| spellingShingle | Jeong Seop Park Jiyuan Piao Gabee Park Hyun Sook Hong Substance-P Restores Cellular Activity of ADSC Impaired by Oxidative Stress Antioxidants Substance-P adipose-derived stem cells oxidative stress paracrine factors |
| title | Substance-P Restores Cellular Activity of ADSC Impaired by Oxidative Stress |
| title_full | Substance-P Restores Cellular Activity of ADSC Impaired by Oxidative Stress |
| title_fullStr | Substance-P Restores Cellular Activity of ADSC Impaired by Oxidative Stress |
| title_full_unstemmed | Substance-P Restores Cellular Activity of ADSC Impaired by Oxidative Stress |
| title_short | Substance-P Restores Cellular Activity of ADSC Impaired by Oxidative Stress |
| title_sort | substance p restores cellular activity of adsc impaired by oxidative stress |
| topic | Substance-P adipose-derived stem cells oxidative stress paracrine factors |
| url | https://www.mdpi.com/2076-3921/9/10/978 |
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