Efficient decolorization of recalcitrant dyes at neutral/alkaline pH by a new bacterial laccase-mediator system
Laccases and laccase-mediator systems (LMS) are versatile catalysts that can oxidize a broad range of substrates coupled to the sole reduction of dioxygen to water. They possess many biotechnological applications in paper, textile, and food industries, bioethanol production, organic synthesis, detec...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-07-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651321003481 |
| _version_ | 1819127896533368832 |
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| author | Lucy L. Coria-Oriundo Fernando Battaglini Sonia A. Wirth |
| author_facet | Lucy L. Coria-Oriundo Fernando Battaglini Sonia A. Wirth |
| author_sort | Lucy L. Coria-Oriundo |
| collection | DOAJ |
| description | Laccases and laccase-mediator systems (LMS) are versatile catalysts that can oxidize a broad range of substrates coupled to the sole reduction of dioxygen to water. They possess many biotechnological applications in paper, textile, and food industries, bioethanol production, organic synthesis, detection and degradation of pollutants, and biofuel cell development. In particular, bacterial laccases are getting relevance due to their activity in a wide range of pH and temperature and their robustness under harsh conditions. However, the enzyme and the redox mediator's availability and costs limit their large-scale commercial use. Here we demonstrate that β-(10-phenothiazyl)-propionic acid can be used as an efficient and low-cost redox mediator for decolorizing synthetic dyes by the recombinant laccase SilA from Streptomyces ipomoeae produced in E. coli. This new LMS can decolorize more than 80% indigo carmine and malachite green in 1 h at pH = 8.0 and 2 h in tap water (pH = 6.8). Furthermore, it decolorized more than 40% of anthraquinone dye remazol brilliant blue R and 80% of azo dye xylidine ponceau in 5 h at 50 °C, pH 8.0. It supported at least 3 decolorization cycles without losing activity, representing an attractive candidate for a cost-effective and environmentally friendly LMS functional at neutral to alkaline pH. |
| first_indexed | 2024-12-22T08:19:13Z |
| format | Article |
| id | doaj.art-db7e2321e33f4bbea09010b4584127fd |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-12-22T08:19:13Z |
| publishDate | 2021-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-db7e2321e33f4bbea09010b4584127fd2022-12-21T18:32:49ZengElsevierEcotoxicology and Environmental Safety0147-65132021-07-01217112237Efficient decolorization of recalcitrant dyes at neutral/alkaline pH by a new bacterial laccase-mediator systemLucy L. Coria-Oriundo0Fernando Battaglini1Sonia A. Wirth2Instituto de Química Física de los Materiales, Medio Ambiente y Energía, INQUIMAE, DQIAQF, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, Ciudad Universitaria, C1428EGA Buenos Aires, Argentina; Facultad de Ciencias, Universidad Nacional de Ingeniería, Av. Tupac Amaru 210, Lima 25, PerúInstituto de Química Física de los Materiales, Medio Ambiente y Energía, INQUIMAE, DQIAQF, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, Ciudad Universitaria, C1428EGA Buenos Aires, ArgentinaLaboratorio de Agrobiotecnología, Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes 2160, Ciudad Universitaria, C1428EGA, Argentina; Instituto de Biodiversidad y Biología Experimental y Aplicada, IBBEA-CONICET-UBA, Intendente Güiraldes 2160, Ciudad Universitaria, C1428EGA, Argentina; Corresponding author at: Instituto de Biodiversidad y Biología Experimental y Aplicada, IBBEA-CONICET-UBA, Intendente Güiraldes 2160, Ciudad Universitaria, C1428EGA, Argentina.Laccases and laccase-mediator systems (LMS) are versatile catalysts that can oxidize a broad range of substrates coupled to the sole reduction of dioxygen to water. They possess many biotechnological applications in paper, textile, and food industries, bioethanol production, organic synthesis, detection and degradation of pollutants, and biofuel cell development. In particular, bacterial laccases are getting relevance due to their activity in a wide range of pH and temperature and their robustness under harsh conditions. However, the enzyme and the redox mediator's availability and costs limit their large-scale commercial use. Here we demonstrate that β-(10-phenothiazyl)-propionic acid can be used as an efficient and low-cost redox mediator for decolorizing synthetic dyes by the recombinant laccase SilA from Streptomyces ipomoeae produced in E. coli. This new LMS can decolorize more than 80% indigo carmine and malachite green in 1 h at pH = 8.0 and 2 h in tap water (pH = 6.8). Furthermore, it decolorized more than 40% of anthraquinone dye remazol brilliant blue R and 80% of azo dye xylidine ponceau in 5 h at 50 °C, pH 8.0. It supported at least 3 decolorization cycles without losing activity, representing an attractive candidate for a cost-effective and environmentally friendly LMS functional at neutral to alkaline pH.http://www.sciencedirect.com/science/article/pii/S0147651321003481PhenothiazineBacillus subtilis CotAStreptomyces ipomoeae SilADye decolorizationLaccase |
| spellingShingle | Lucy L. Coria-Oriundo Fernando Battaglini Sonia A. Wirth Efficient decolorization of recalcitrant dyes at neutral/alkaline pH by a new bacterial laccase-mediator system Ecotoxicology and Environmental Safety Phenothiazine Bacillus subtilis CotA Streptomyces ipomoeae SilA Dye decolorization Laccase |
| title | Efficient decolorization of recalcitrant dyes at neutral/alkaline pH by a new bacterial laccase-mediator system |
| title_full | Efficient decolorization of recalcitrant dyes at neutral/alkaline pH by a new bacterial laccase-mediator system |
| title_fullStr | Efficient decolorization of recalcitrant dyes at neutral/alkaline pH by a new bacterial laccase-mediator system |
| title_full_unstemmed | Efficient decolorization of recalcitrant dyes at neutral/alkaline pH by a new bacterial laccase-mediator system |
| title_short | Efficient decolorization of recalcitrant dyes at neutral/alkaline pH by a new bacterial laccase-mediator system |
| title_sort | efficient decolorization of recalcitrant dyes at neutral alkaline ph by a new bacterial laccase mediator system |
| topic | Phenothiazine Bacillus subtilis CotA Streptomyces ipomoeae SilA Dye decolorization Laccase |
| url | http://www.sciencedirect.com/science/article/pii/S0147651321003481 |
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