Immunoprofiling of fresh HAM/TSP blood samples shows altered innate cell responsiveness.
The Human T-cell Leukemia Virus-1 (HTLV-1)-Associated Myelopathy/Tropical Spastic Paraparesis (HAM/TSP) is a devastating neurodegenerative disease with no effective treatment, which affects an increasing number of people in Brazil. Immune cells from the adaptive compartment are involved in disease m...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-11-01
|
| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://doi.org/10.1371/journal.pntd.0009940 |
| _version_ | 1798035407367045120 |
|---|---|
| author | Brenda Rocamonde Nicolas Futsch Noemia Orii Omran Allatif Augusto Cesar Penalva de Oliveira Renaud Mahieux Jorge Casseb Hélène Dutartre |
| author_facet | Brenda Rocamonde Nicolas Futsch Noemia Orii Omran Allatif Augusto Cesar Penalva de Oliveira Renaud Mahieux Jorge Casseb Hélène Dutartre |
| author_sort | Brenda Rocamonde |
| collection | DOAJ |
| description | The Human T-cell Leukemia Virus-1 (HTLV-1)-Associated Myelopathy/Tropical Spastic Paraparesis (HAM/TSP) is a devastating neurodegenerative disease with no effective treatment, which affects an increasing number of people in Brazil. Immune cells from the adaptive compartment are involved in disease manifestation but whether innate cell functions participate in disease occurrence has not been evaluated. In this study, we analyzed innate cell responses at steady state and after blood cell stimulation using an agonist of the toll-like receptor (TLR)7/8-signaling pathway in blood samples from HTLV-1-infected volunteers, including asymptomatic carriers and HAM/TSP patients. We observed a lower response of IFNα+ DCs and monocytes in HAM/TSP compared to asymptomatic carriers, as a potential consequence of corticosteroid treatments. In contrast, a higher frequency of monocytes producing MIP-1α and pDC producing IL-12 was detected in HAM/TSP blood samples, together with higher IFNγ responsiveness of NK cells, suggesting an increased sensitivity to inflammatory response in HAM/TSP patients compared to asymptomatic carriers. This sustained inflammatory responsiveness could be linked or be at the origin of the neuroinflammatory status in HAM/TSP patients. Therefore, the mechanism underlying this dysregulations could shed light onto the origins of HAM/TSP disease. |
| first_indexed | 2024-04-11T20:57:46Z |
| format | Article |
| id | doaj.art-db81415417f94efdb20bd8811409b193 |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2024-04-11T20:57:46Z |
| publishDate | 2021-11-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-db81415417f94efdb20bd8811409b1932022-12-22T04:03:38ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352021-11-011511e000994010.1371/journal.pntd.0009940Immunoprofiling of fresh HAM/TSP blood samples shows altered innate cell responsiveness.Brenda RocamondeNicolas FutschNoemia OriiOmran AllatifAugusto Cesar Penalva de OliveiraRenaud MahieuxJorge CassebHélène DutartreThe Human T-cell Leukemia Virus-1 (HTLV-1)-Associated Myelopathy/Tropical Spastic Paraparesis (HAM/TSP) is a devastating neurodegenerative disease with no effective treatment, which affects an increasing number of people in Brazil. Immune cells from the adaptive compartment are involved in disease manifestation but whether innate cell functions participate in disease occurrence has not been evaluated. In this study, we analyzed innate cell responses at steady state and after blood cell stimulation using an agonist of the toll-like receptor (TLR)7/8-signaling pathway in blood samples from HTLV-1-infected volunteers, including asymptomatic carriers and HAM/TSP patients. We observed a lower response of IFNα+ DCs and monocytes in HAM/TSP compared to asymptomatic carriers, as a potential consequence of corticosteroid treatments. In contrast, a higher frequency of monocytes producing MIP-1α and pDC producing IL-12 was detected in HAM/TSP blood samples, together with higher IFNγ responsiveness of NK cells, suggesting an increased sensitivity to inflammatory response in HAM/TSP patients compared to asymptomatic carriers. This sustained inflammatory responsiveness could be linked or be at the origin of the neuroinflammatory status in HAM/TSP patients. Therefore, the mechanism underlying this dysregulations could shed light onto the origins of HAM/TSP disease.https://doi.org/10.1371/journal.pntd.0009940 |
| spellingShingle | Brenda Rocamonde Nicolas Futsch Noemia Orii Omran Allatif Augusto Cesar Penalva de Oliveira Renaud Mahieux Jorge Casseb Hélène Dutartre Immunoprofiling of fresh HAM/TSP blood samples shows altered innate cell responsiveness. PLoS Neglected Tropical Diseases |
| title | Immunoprofiling of fresh HAM/TSP blood samples shows altered innate cell responsiveness. |
| title_full | Immunoprofiling of fresh HAM/TSP blood samples shows altered innate cell responsiveness. |
| title_fullStr | Immunoprofiling of fresh HAM/TSP blood samples shows altered innate cell responsiveness. |
| title_full_unstemmed | Immunoprofiling of fresh HAM/TSP blood samples shows altered innate cell responsiveness. |
| title_short | Immunoprofiling of fresh HAM/TSP blood samples shows altered innate cell responsiveness. |
| title_sort | immunoprofiling of fresh ham tsp blood samples shows altered innate cell responsiveness |
| url | https://doi.org/10.1371/journal.pntd.0009940 |
| work_keys_str_mv | AT brendarocamonde immunoprofilingoffreshhamtspbloodsamplesshowsalteredinnatecellresponsiveness AT nicolasfutsch immunoprofilingoffreshhamtspbloodsamplesshowsalteredinnatecellresponsiveness AT noemiaorii immunoprofilingoffreshhamtspbloodsamplesshowsalteredinnatecellresponsiveness AT omranallatif immunoprofilingoffreshhamtspbloodsamplesshowsalteredinnatecellresponsiveness AT augustocesarpenalvadeoliveira immunoprofilingoffreshhamtspbloodsamplesshowsalteredinnatecellresponsiveness AT renaudmahieux immunoprofilingoffreshhamtspbloodsamplesshowsalteredinnatecellresponsiveness AT jorgecasseb immunoprofilingoffreshhamtspbloodsamplesshowsalteredinnatecellresponsiveness AT helenedutartre immunoprofilingoffreshhamtspbloodsamplesshowsalteredinnatecellresponsiveness |