Microfluidic Based Physical Approaches towards Single-Cell Intracellular Delivery and Analysis
The ability to deliver foreign molecules into a single living cell with high transfection efficiency and high cell viability is of great interest in cell biology for applications in therapeutic development, diagnostics, and drug delivery towards personalized medicine. Various physical delivery metho...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/12/6/631 |
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| author | Kiran Kaladharan Ashish Kumar Pallavi Gupta Kavitha Illath Tuhin Subhra Santra Fan-Gang Tseng |
| author_facet | Kiran Kaladharan Ashish Kumar Pallavi Gupta Kavitha Illath Tuhin Subhra Santra Fan-Gang Tseng |
| author_sort | Kiran Kaladharan |
| collection | DOAJ |
| description | The ability to deliver foreign molecules into a single living cell with high transfection efficiency and high cell viability is of great interest in cell biology for applications in therapeutic development, diagnostics, and drug delivery towards personalized medicine. Various physical delivery methods have long demonstrated the ability to deliver cargo molecules directly to the cytoplasm or nucleus and the mechanisms underlying most of the approaches have been extensively investigated. However, most of these techniques are bulk approaches that are cell-specific and have low throughput delivery. In comparison to bulk measurements, single-cell measurement technologies can provide a better understanding of the interactions among molecules, organelles, cells, and the microenvironment, which can aid in the development of therapeutics and diagnostic tools. To elucidate distinct responses during cell genetic modification, methods to achieve transfection at the single-cell level are of great interest. In recent years, single-cell technologies have become increasingly robust and accessible, although limitations exist. This review article aims to cover various microfluidic-based physical methods for single-cell intracellular delivery such as electroporation, mechanoporation, microinjection, sonoporation, optoporation, magnetoporation, and thermoporation and their analysis. The mechanisms of various physical methods, their applications, limitations, and prospects are also elaborated. |
| first_indexed | 2024-03-10T10:55:02Z |
| format | Article |
| id | doaj.art-db89a579034f46eab3e4d013d738363c |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T10:55:02Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-db89a579034f46eab3e4d013d738363c2023-11-21T21:56:53ZengMDPI AGMicromachines2072-666X2021-05-0112663110.3390/mi12060631Microfluidic Based Physical Approaches towards Single-Cell Intracellular Delivery and AnalysisKiran Kaladharan0Ashish Kumar1Pallavi Gupta2Kavitha Illath3Tuhin Subhra Santra4Fan-Gang Tseng5Department of Engineering and System Science, National Tsing Hua University, Hsinchu 300044, TaiwanDepartment of Engineering and System Science, National Tsing Hua University, Hsinchu 300044, TaiwanDepartment of Engineering Design, Indian Institute of Technology Madras, Chennai 600036, IndiaDepartment of Engineering Design, Indian Institute of Technology Madras, Chennai 600036, IndiaDepartment of Engineering Design, Indian Institute of Technology Madras, Chennai 600036, IndiaDepartment of Engineering and System Science, National Tsing Hua University, Hsinchu 300044, TaiwanThe ability to deliver foreign molecules into a single living cell with high transfection efficiency and high cell viability is of great interest in cell biology for applications in therapeutic development, diagnostics, and drug delivery towards personalized medicine. Various physical delivery methods have long demonstrated the ability to deliver cargo molecules directly to the cytoplasm or nucleus and the mechanisms underlying most of the approaches have been extensively investigated. However, most of these techniques are bulk approaches that are cell-specific and have low throughput delivery. In comparison to bulk measurements, single-cell measurement technologies can provide a better understanding of the interactions among molecules, organelles, cells, and the microenvironment, which can aid in the development of therapeutics and diagnostic tools. To elucidate distinct responses during cell genetic modification, methods to achieve transfection at the single-cell level are of great interest. In recent years, single-cell technologies have become increasingly robust and accessible, although limitations exist. This review article aims to cover various microfluidic-based physical methods for single-cell intracellular delivery such as electroporation, mechanoporation, microinjection, sonoporation, optoporation, magnetoporation, and thermoporation and their analysis. The mechanisms of various physical methods, their applications, limitations, and prospects are also elaborated.https://www.mdpi.com/2072-666X/12/6/631physical methods for intracellular deliverysingle-cellmicrofluidic devicestransfection efficiencycell viability |
| spellingShingle | Kiran Kaladharan Ashish Kumar Pallavi Gupta Kavitha Illath Tuhin Subhra Santra Fan-Gang Tseng Microfluidic Based Physical Approaches towards Single-Cell Intracellular Delivery and Analysis Micromachines physical methods for intracellular delivery single-cell microfluidic devices transfection efficiency cell viability |
| title | Microfluidic Based Physical Approaches towards Single-Cell Intracellular Delivery and Analysis |
| title_full | Microfluidic Based Physical Approaches towards Single-Cell Intracellular Delivery and Analysis |
| title_fullStr | Microfluidic Based Physical Approaches towards Single-Cell Intracellular Delivery and Analysis |
| title_full_unstemmed | Microfluidic Based Physical Approaches towards Single-Cell Intracellular Delivery and Analysis |
| title_short | Microfluidic Based Physical Approaches towards Single-Cell Intracellular Delivery and Analysis |
| title_sort | microfluidic based physical approaches towards single cell intracellular delivery and analysis |
| topic | physical methods for intracellular delivery single-cell microfluidic devices transfection efficiency cell viability |
| url | https://www.mdpi.com/2072-666X/12/6/631 |
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