Microfluidic Based Physical Approaches towards Single-Cell Intracellular Delivery and Analysis

Microfluidic Based Physical Approaches towards Single-Cell Intracellular Delivery and Analysis

The ability to deliver foreign molecules into a single living cell with high transfection efficiency and high cell viability is of great interest in cell biology for applications in therapeutic development, diagnostics, and drug delivery towards personalized medicine. Various physical delivery metho...

Full description

Bibliographic Details
Main Authors:	Kiran Kaladharan, Ashish Kumar, Pallavi Gupta, Kavitha Illath, Tuhin Subhra Santra, Fan-Gang Tseng
Format:	Article
Language:	English
Published:	MDPI AG 2021-05-01
Series:	Micromachines
Subjects:	physical methods for intracellular delivery single-cell microfluidic devices transfection efficiency cell viability
Online Access:	https://www.mdpi.com/2072-666X/12/6/631

Similar Items

Microfluidic mechanoporation for cellular delivery and analysis
by: Pulasta Chakrabarty, et al.
Published: (2022-01-01)

Microfluidic and Nanofluidic Intracellular Delivery
by: Jeongsoo Hur, et al.
Published: (2021-08-01)

Microfluidic platforms for single neuron analysis
by: Pallavi Gupta, et al.
Published: (2022-01-01)

Laser-Induced Intracellular Delivery: Exploiting Gold-Coated Spiky Polymeric Nanoparticles and Gold Nanorods under Near-Infrared Pulses for Single-Cell Nano-Photon-Poration
by: Ashish Kumar, et al.
Published: (2024-01-01)

Electroactive nanoinjection platform for intracellular delivery and gene silencing
by: Ali-Reza Shokouhi, et al.
Published: (2023-08-01)

A high‐throughput microfluidic mechanoporation platform to enable intracellular delivery of cyclic peptides in cell‐based assays
by: Stephen H. Kasper, et al.
Published: (2023-09-01)

Recent Trends on Micro/Nanofluidic Single Cell Electroporation
by: Tuhin Subhra Santra, et al.
Published: (2013-09-01)

Microfluidic Formulation of DNA-Loaded Multicomponent Lipid Nanoparticles for Gene Delivery
by: Erica Quagliarini, et al.
Published: (2021-08-01)

Single cell transfection of human-induced pluripotent stem cells using a droplet-based microfluidic system
by: Camilo Pérez-Sosa, et al.
Published: (2022-01-01)

Microfluidic Devices in Advanced Caenorhabditis elegans Research
by: Muniesh Muthaiyan Shanmugam, et al.
Published: (2016-08-01)

Intracellular Delivery of DNA and Protein by a Novel Cell-Permeable Peptide Derived from DOT1L
by: Jingping Geng, et al.
Published: (2020-02-01)

Targeted Intracellular Delivery of Antibodies: The State of the Art
by: Tatiana A. Slastnikova, et al.
Published: (2018-10-01)

A Microfluidic System of Gene Transfer by Ultrasound
by: Cuimin Sun, et al.
Published: (2022-07-01)

Intracellular Delivery of Active Proteins by Polyphosphazene Polymers
by: Bareera Qamar, et al.
Published: (2021-02-01)

Association between adeno‐associated virus genomic titers and intracellular plasmid levels
by: Kyoko Masumi‐Koizumi, et al.
Published: (2024-01-01)

A Review of Single-Cell Adhesion Force Kinetics and Applications
by: Ashwini Shinde, et al.
Published: (2021-03-01)

Microfluidic Analyzer Enabling Quantitative Measurements of Specific Intracellular Proteins at the Single-Cell Level
by: Lixing Liu, et al.
Published: (2018-11-01)

Rapid and Highly Sensitive Electrochemical Technique for Cell Viability Assay via Monitoring of Intracellular NADH with New Double Mediator System
by: Mohammad Abdul ALIM, et al.
Published: (2021-11-01)

Cell Adhesion, Morphology, and Metabolism Variation via Acoustic Exposure within Microfluidic Cell Handling Systems
by: Citsabehsan Devendran, et al.
Published: (2019-12-01)

Effect of inserted spacer in hepatic cell-penetrating multifunctional peptide component on the DNA intracellular delivery of quaternary complexes based on modular design
by: Zhang L, et al.
Published: (2016-11-01)

Three-Dimensional Printing Enabled Droplet Microfluidic Device for Real-Time Monitoring of Single-Cell Viability and Blebbing Activity
by: Meiai Lin, et al.
Published: (2023-07-01)

A Microfluidic Probe Integrated Device for Spatiotemporal 3D Chemical Stimulation in Cells
by: Kenta Shinha, et al.
Published: (2020-07-01)

Response Surface Methodology to Efficiently Optimize Intracellular Delivery by Photoporation
by: Ilia Goemaere, et al.
Published: (2023-02-01)

Highly integrated microfluidics design /
by: Angelescu, Dan E., 1976-
Published: (c201)

Microfluidics for biological applications /
by: Tian, Wei-Cheng, et al.
Published: (2008)

Flavonoids Enhance Lipofection Efficiency and Ameliorate Cytotoxicity in Colon26 and HepG2 Cells via Oxidative Stress Regulation
by: Die Hu, et al.
Published: (2022-06-01)

Coupling of nanostraws with diverse physicochemical perforation strategies for intracellular DNA delivery
by: Juan Jiang, et al.
Published: (2024-03-01)

Serum Protects Cells and Increases Intracellular Delivery of Molecules by Nanoparticle-Mediated Photoporation
by: Kumar S, et al.
Published: (2021-05-01)

A Review on Electroporation-Based Intracellular Delivery
by: Junfeng Shi, et al.
Published: (2018-11-01)

Liposome-based Formulation for Intracellular Delivery of Functional Proteins
by: Benoît Chatin, et al.
Published: (2015-01-01)

Quantitative Image-Based Cell Viability (QuantICV) Assay for Microfluidic 3D Tissue Culture Applications
by: Louis Jun Ye Ong, et al.
Published: (2020-07-01)

Microfluidic cell-based assay using cotton fabrics /
by: Salasiah Mohamad, 1991-, author, et al.
Published: (2014)

A Microfluidic System for the Investigation of Tumor Cell Extravasation
by: Claudia Kühlbach, et al.
Published: (2018-05-01)

Efficient and scalable gene delivery method with easily generated cationic carbon dots
by: Manuel Algarra, et al.
Published: (2024-03-01)

Towards Intracellular Delivery of Peptides
by: Andreas L. Marzinzik, et al.
Published: (2013-12-01)

A solution for highly efficient electroporation of primary cytotoxic T lymphocytes
by: Nadia Alawar, et al.
Published: (2024-03-01)

Single-Cell Electroporation with Real-Time Impedance Assessment Using a Constriction Microchannel
by: Yifei Ye, et al.
Published: (2020-09-01)

Electric and Electrochemical Microfluidic Devices for Cell Analysis
by: Kaoru Hiramoto, et al.
Published: (2019-06-01)

Microfluidics and nanofluidics handbook : fabrication, implementation, and applications /
by: Mitra, Sushanta K., et al.
Published: (c201)

<i>N</i>-[4-(<i>N,N,N</i>-Trimethylammonium)Benzyl]Chitosan Chloride as a Gene Carrier: The Influence of Polyplex Composition and Cell Type
by: Sergei V. Raik, et al.
Published: (2021-05-01)