Prognostic Implications of MALAT1 and BACH1 Expression and Their Correlation with CTCs and Mo-MDSCs in Triple Negative Breast Cancer and Surgical Management Options
Background. Triple negative breast cancer (TNBC) is a biologically separate entity of breast cancer that cannot get benefits from targeted or endocrine therapy. Objective. To assess the expression of MALAT1 and BACH1, as well as monocyte-myeloid-derived suppressor cell (Mo-MDSC) levels and circulati...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hindawi Limited
2022-01-01
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| Series: | International Journal of Breast Cancer |
| Online Access: | http://dx.doi.org/10.1155/2022/8096764 |
| _version_ | 1798025044813676544 |
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| author | Samah Said Elbasateeny Mahmoud Abdou Yassin Mohamed Mahmoud Mokhtar Adel Mohamed Ismail Huda Fathy Ebian Samia Hussein Sherin Attia Shazly Mai Mohammed Abdelwabab |
| author_facet | Samah Said Elbasateeny Mahmoud Abdou Yassin Mohamed Mahmoud Mokhtar Adel Mohamed Ismail Huda Fathy Ebian Samia Hussein Sherin Attia Shazly Mai Mohammed Abdelwabab |
| author_sort | Samah Said Elbasateeny |
| collection | DOAJ |
| description | Background. Triple negative breast cancer (TNBC) is a biologically separate entity of breast cancer that cannot get benefits from targeted or endocrine therapy. Objective. To assess the expression of MALAT1 and BACH1, as well as monocyte-myeloid-derived suppressor cell (Mo-MDSC) levels and circulating tumor cell (CTC) count in TNBC to correlate these markers with the clinic-pathological criteria of TNCB patients and to evaluate their roles as predictive markers for selection of the patients that can be operated by oncoplastic conserving breast surgery. Methods. Eighty-eight TNBC were managed by modified doughnut breast oncoplastic surgery in early stages and by modified radical mastectomy for patients with late stages unsuitable for breast-conserving. All were examined for MALAT1 and BACH1 expression by immunohistochemistry and RT-PCR as well as Mo-MDSC levels and CTCs. Results. MALAT1 and BACH1 expressions are correlated with the larger size, lymph node, distance metastasis, and TNM staging (p<0.05). CTCs≥5 and high MO-MDSCs were significantly more in TNBC with MALAT1 and BACH1 overexpression. The survival study proved that DFS for patients with both positive expression of MALAT1 and BACH1 was shorter than that of one positive expression, and both negative expression p≤0.001, CTCs≥5, and high Mo-MDSCs are associated with poor outcomes. No significant difference between modified round block and modified radical mastectomy techniques as regards recurrence. However, all postoperative management outcomes were significantly better in patients operated by oncoplastic conserving breast surgery. Conclusion. BACH1 and MALAT1 expressions are significantly upregulated in TNBC. They are correlated with CTCs and Mo-MDCs, and all are associated with poor outcomes. Not all TNBC patients have a bad prognosis, patients negative for one of MALAT1 and BACH1 or both, have a slightly good prognosis, and so can be managed by breast oncoplastic conserving surgery. |
| first_indexed | 2024-04-11T18:12:28Z |
| format | Article |
| id | doaj.art-db8c21f43dd94e0ba9910c1ac4b5ddba |
| institution | Directory Open Access Journal |
| issn | 2090-3189 |
| language | English |
| last_indexed | 2024-04-11T18:12:28Z |
| publishDate | 2022-01-01 |
| publisher | Hindawi Limited |
| record_format | Article |
| series | International Journal of Breast Cancer |
| spelling | doaj.art-db8c21f43dd94e0ba9910c1ac4b5ddba2022-12-22T04:10:04ZengHindawi LimitedInternational Journal of Breast Cancer2090-31892022-01-01202210.1155/2022/8096764Prognostic Implications of MALAT1 and BACH1 Expression and Their Correlation with CTCs and Mo-MDSCs in Triple Negative Breast Cancer and Surgical Management OptionsSamah Said Elbasateeny0Mahmoud Abdou Yassin1Mohamed Mahmoud Mokhtar2Adel Mohamed Ismail3Huda Fathy Ebian4Samia Hussein5Sherin Attia Shazly6Mai Mohammed Abdelwabab7Pathology DepartmentGeneral Surgery DepartmentGeneral Surgery DepartmentSurgical Oncology DepartmentClinical Pathology DepartmentMedical Biochemistry and Molecular Biology DepartmentObstetrics and Gynecology DepartmentPathology DepartmentBackground. Triple negative breast cancer (TNBC) is a biologically separate entity of breast cancer that cannot get benefits from targeted or endocrine therapy. Objective. To assess the expression of MALAT1 and BACH1, as well as monocyte-myeloid-derived suppressor cell (Mo-MDSC) levels and circulating tumor cell (CTC) count in TNBC to correlate these markers with the clinic-pathological criteria of TNCB patients and to evaluate their roles as predictive markers for selection of the patients that can be operated by oncoplastic conserving breast surgery. Methods. Eighty-eight TNBC were managed by modified doughnut breast oncoplastic surgery in early stages and by modified radical mastectomy for patients with late stages unsuitable for breast-conserving. All were examined for MALAT1 and BACH1 expression by immunohistochemistry and RT-PCR as well as Mo-MDSC levels and CTCs. Results. MALAT1 and BACH1 expressions are correlated with the larger size, lymph node, distance metastasis, and TNM staging (p<0.05). CTCs≥5 and high MO-MDSCs were significantly more in TNBC with MALAT1 and BACH1 overexpression. The survival study proved that DFS for patients with both positive expression of MALAT1 and BACH1 was shorter than that of one positive expression, and both negative expression p≤0.001, CTCs≥5, and high Mo-MDSCs are associated with poor outcomes. No significant difference between modified round block and modified radical mastectomy techniques as regards recurrence. However, all postoperative management outcomes were significantly better in patients operated by oncoplastic conserving breast surgery. Conclusion. BACH1 and MALAT1 expressions are significantly upregulated in TNBC. They are correlated with CTCs and Mo-MDCs, and all are associated with poor outcomes. Not all TNBC patients have a bad prognosis, patients negative for one of MALAT1 and BACH1 or both, have a slightly good prognosis, and so can be managed by breast oncoplastic conserving surgery.http://dx.doi.org/10.1155/2022/8096764 |
| spellingShingle | Samah Said Elbasateeny Mahmoud Abdou Yassin Mohamed Mahmoud Mokhtar Adel Mohamed Ismail Huda Fathy Ebian Samia Hussein Sherin Attia Shazly Mai Mohammed Abdelwabab Prognostic Implications of MALAT1 and BACH1 Expression and Their Correlation with CTCs and Mo-MDSCs in Triple Negative Breast Cancer and Surgical Management Options International Journal of Breast Cancer |
| title | Prognostic Implications of MALAT1 and BACH1 Expression and Their Correlation with CTCs and Mo-MDSCs in Triple Negative Breast Cancer and Surgical Management Options |
| title_full | Prognostic Implications of MALAT1 and BACH1 Expression and Their Correlation with CTCs and Mo-MDSCs in Triple Negative Breast Cancer and Surgical Management Options |
| title_fullStr | Prognostic Implications of MALAT1 and BACH1 Expression and Their Correlation with CTCs and Mo-MDSCs in Triple Negative Breast Cancer and Surgical Management Options |
| title_full_unstemmed | Prognostic Implications of MALAT1 and BACH1 Expression and Their Correlation with CTCs and Mo-MDSCs in Triple Negative Breast Cancer and Surgical Management Options |
| title_short | Prognostic Implications of MALAT1 and BACH1 Expression and Their Correlation with CTCs and Mo-MDSCs in Triple Negative Breast Cancer and Surgical Management Options |
| title_sort | prognostic implications of malat1 and bach1 expression and their correlation with ctcs and mo mdscs in triple negative breast cancer and surgical management options |
| url | http://dx.doi.org/10.1155/2022/8096764 |
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