Early Succession of Community Structures and Biotic Interactions of Gut Microbes in <i>Eriocheir sinensis</i> Megalopa after Desalination
As an enduring Chinese freshwater aquaculture product, the <i>Eriocheir sinensis</i> has a high economic value and is characterized by a catadromous life style that undergoes seawater–freshwater migration. However, little is known about their gut microbial status as they move from saltwa...
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| Format: | Article |
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MDPI AG
2024-03-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/12/3/560 |
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| author | Wenlei Xue Hao Wu Xinyu Wu Nannan Li Ximei Nie Tianheng Gao |
| author_facet | Wenlei Xue Hao Wu Xinyu Wu Nannan Li Ximei Nie Tianheng Gao |
| author_sort | Wenlei Xue |
| collection | DOAJ |
| description | As an enduring Chinese freshwater aquaculture product, the <i>Eriocheir sinensis</i> has a high economic value and is characterized by a catadromous life style that undergoes seawater–freshwater migration. However, little is known about their gut microbial status as they move from saltwater to freshwater acclimatization. Here, we sampled and cultivated <i>Eriocheir sinensis</i> megalopa from three aquaculture desalination ponds and investigated their gut microbiota diversity, community structures and biotic interactions from megalopa stage to the first juvenile stage after desalination for 9 days. Our results revealed that during the transition from megalopa to the first juvenile in <i>Eriocheir sinensis</i>, a significant change in gut microbial composition was observed (for instance, changes in relative abundance of dominant phyla), which was, however, not influenced by different sampling sites. The species diversity (such as the richness) of the gut microbiota showed a hump-shaped pattern along the succession. However, the compositional differences of the gut microbes showed constantly increasing patterns during the succession after freshwater adaption for all three sampling sites. Further co-occurrence analysis also showed that the complexity of the ecological networks in gut microbes was significantly enhanced during the development, such as increasing numbers of network links, connectivity and modularity, and was confirmed by decreasing average path length and proportions of negative links. Taken together, the differences in community structures and biological interactions of gut microorganisms were more pronounced in <i>Eriocheir sinensis</i> megalopa during desalination than in diversity and species compositions. This implies that the gut microbes of <i>Eriocheir sinensis</i> megalopa would become more robust and adaptive during the developmental process. |
| first_indexed | 2024-04-24T17:59:51Z |
| format | Article |
| id | doaj.art-db8ff6e6987b474fb4bcc6bc0bd75eb0 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-04-24T17:59:51Z |
| publishDate | 2024-03-01 |
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| series | Microorganisms |
| spelling | doaj.art-db8ff6e6987b474fb4bcc6bc0bd75eb02024-03-27T13:55:50ZengMDPI AGMicroorganisms2076-26072024-03-0112356010.3390/microorganisms12030560Early Succession of Community Structures and Biotic Interactions of Gut Microbes in <i>Eriocheir sinensis</i> Megalopa after DesalinationWenlei Xue0Hao Wu1Xinyu Wu2Nannan Li3Ximei Nie4Tianheng Gao5Jiangsu Province Engineering Research Center for Marine Bio-Resources Sustainable Utilization, College of Oceanography, Hohai University, Nanjing 210024, ChinaJiangsu Province Engineering Research Center for Marine Bio-Resources Sustainable Utilization, College of Oceanography, Hohai University, Nanjing 210024, ChinaJiangsu Province Engineering Research Center for Marine Bio-Resources Sustainable Utilization, College of Oceanography, Hohai University, Nanjing 210024, ChinaJiangsu Province Engineering Research Center for Marine Bio-Resources Sustainable Utilization, College of Oceanography, Hohai University, Nanjing 210024, ChinaJiangsu Province Engineering Research Center for Aquatic Animals Breeding and Green Efficient Aquacultural Technology, College of Marine Science and Engineering, Nanjing Normal University, Nanjing 210023, ChinaJiangsu Province Engineering Research Center for Marine Bio-Resources Sustainable Utilization, College of Oceanography, Hohai University, Nanjing 210024, ChinaAs an enduring Chinese freshwater aquaculture product, the <i>Eriocheir sinensis</i> has a high economic value and is characterized by a catadromous life style that undergoes seawater–freshwater migration. However, little is known about their gut microbial status as they move from saltwater to freshwater acclimatization. Here, we sampled and cultivated <i>Eriocheir sinensis</i> megalopa from three aquaculture desalination ponds and investigated their gut microbiota diversity, community structures and biotic interactions from megalopa stage to the first juvenile stage after desalination for 9 days. Our results revealed that during the transition from megalopa to the first juvenile in <i>Eriocheir sinensis</i>, a significant change in gut microbial composition was observed (for instance, changes in relative abundance of dominant phyla), which was, however, not influenced by different sampling sites. The species diversity (such as the richness) of the gut microbiota showed a hump-shaped pattern along the succession. However, the compositional differences of the gut microbes showed constantly increasing patterns during the succession after freshwater adaption for all three sampling sites. Further co-occurrence analysis also showed that the complexity of the ecological networks in gut microbes was significantly enhanced during the development, such as increasing numbers of network links, connectivity and modularity, and was confirmed by decreasing average path length and proportions of negative links. Taken together, the differences in community structures and biological interactions of gut microorganisms were more pronounced in <i>Eriocheir sinensis</i> megalopa during desalination than in diversity and species compositions. This implies that the gut microbes of <i>Eriocheir sinensis</i> megalopa would become more robust and adaptive during the developmental process.https://www.mdpi.com/2076-2607/12/3/560<i>Eriocheir sinensis</i> megalpopagut microbiotalarval developmentbacterial communitygut co-occurrence network |
| spellingShingle | Wenlei Xue Hao Wu Xinyu Wu Nannan Li Ximei Nie Tianheng Gao Early Succession of Community Structures and Biotic Interactions of Gut Microbes in <i>Eriocheir sinensis</i> Megalopa after Desalination Microorganisms <i>Eriocheir sinensis</i> megalpopa gut microbiota larval development bacterial community gut co-occurrence network |
| title | Early Succession of Community Structures and Biotic Interactions of Gut Microbes in <i>Eriocheir sinensis</i> Megalopa after Desalination |
| title_full | Early Succession of Community Structures and Biotic Interactions of Gut Microbes in <i>Eriocheir sinensis</i> Megalopa after Desalination |
| title_fullStr | Early Succession of Community Structures and Biotic Interactions of Gut Microbes in <i>Eriocheir sinensis</i> Megalopa after Desalination |
| title_full_unstemmed | Early Succession of Community Structures and Biotic Interactions of Gut Microbes in <i>Eriocheir sinensis</i> Megalopa after Desalination |
| title_short | Early Succession of Community Structures and Biotic Interactions of Gut Microbes in <i>Eriocheir sinensis</i> Megalopa after Desalination |
| title_sort | early succession of community structures and biotic interactions of gut microbes in i eriocheir sinensis i megalopa after desalination |
| topic | <i>Eriocheir sinensis</i> megalpopa gut microbiota larval development bacterial community gut co-occurrence network |
| url | https://www.mdpi.com/2076-2607/12/3/560 |
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