Effects of additional gonadotropin-releasing hormone and prostaglandin F2α treatment to an estradiol/progesterone-based embryo transfer protocol for recipient lactating dairy cows
ABSTRACT: This study was designed to evaluate whether the utilization of a second PGF2α treatment at the end of an estradiol/progesterone (E2/P4)-based protocol with or without GnRH at the beginning of the protocol would improve pregnancy rates of lactating Holstein cows assigned to timed embryo tra...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-02-01
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| Series: | Journal of Dairy Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022030222006956 |
| _version_ | 1797946394986676224 |
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| author | M.H.C. Pereira B.I. Cappellozza R.L.A. Cerri C.P. Sanches, Jr. T.G. Guida L.F.S.P. Barbosa J.E.P. Santos J.L.M. Vasconcelos |
| author_facet | M.H.C. Pereira B.I. Cappellozza R.L.A. Cerri C.P. Sanches, Jr. T.G. Guida L.F.S.P. Barbosa J.E.P. Santos J.L.M. Vasconcelos |
| author_sort | M.H.C. Pereira |
| collection | DOAJ |
| description | ABSTRACT: This study was designed to evaluate whether the utilization of a second PGF2α treatment at the end of an estradiol/progesterone (E2/P4)-based protocol with or without GnRH at the beginning of the protocol would improve pregnancy rates of lactating Holstein cows assigned to timed embryo transfer. A total of 501 lactating Holstein cows in 5 farms were enrolled in the experiment. Within farm, cows were blocked by parity and, within block, were assigned randomly to (1) insertion of an intravaginal P4 device (controlled internal drug-releasing device; CIDR) and estradiol benzoate on d −11, PGF2α on d −4, CIDR withdrawal and an injection of estradiol cypionate on d −2, and timed embryo transfer on d 7 (1-PGF; n = 164); (2) the same treatments as 1-PGF, but with PGF2α administered on d −4 and −2 (2-PGF; n = 171); and (3) 2-PGF with the addition of a GnRH treatment on d −11 (GnRH+2-PGF; n = 166). Ovaries were scanned by transrectal ultrasonography on d −11, −4, and 7, and blood samples were collected on d −11, −4, 0, and 7 for P4 determination. Treatment comparisons were performed using contrasts. The proportion of cows with a new corpus luteum on d −4 was greater in GnRH+2-PGF cows. Cows in 1-PGF had a greater P4 concentration on d 0 but lesser P4 on d 7 compared with cows in the other groups. Cows assigned to receive 2-PGF (2-PGF and GnRH+2-PGF) had greater estrus expression, and a greater proportion of cows ovulated to estradiol cypionate. No further contrast effects were observed for follicle diameter, double ovulation rate, pregnancy per embryo transfer (P/ET) on d 32 and 60, or pregnancy loss. As P4 concentration on d −4 increased, P/ET on d 60 tended to increase. Cows with P4 ≥3.66 ng/mL on d −4 had greater P/ET on d 32 and 60 than those with P4 below that threshold. Regardless of treatment, cows with P4 concentration ≥3.66 ng/mL also had a greater pregnancy per synchronized protocol (P/SP) on d 60. Also, a P4 concentration on d −4 (low or high) × follicle diameter (continuous) interaction tendency was observed when evaluating P/ET. Although P/ET did not differ among cows with different follicles sizes with reduced P4 concentration on d −4 (<3.66 ng/mL), it increased in cows with larger follicles exposed to increased P4 concentration (≥3.66 ng/mL). When P4 on d 0 was evaluated, P/ET on d 32 and 60 was greater for cows with low (≤0.09 ng/mL) versus high (>0.21 ng/mL) P4; as P4 concentration on d 0 increased, P/ET linearly decreased. In summary, cows with increased P4 concentrations during growth of the ovulatory follicular wave had improved P/ET. Administering a second PGF2α dose reduced P4 concentration on d 0 and increased ovulatory response to the protocol, but no benefits were observed on P/ET or P/SP. |
| first_indexed | 2024-04-10T21:10:21Z |
| format | Article |
| id | doaj.art-db910f5be01a45b19212c75abea04196 |
| institution | Directory Open Access Journal |
| issn | 0022-0302 |
| language | English |
| last_indexed | 2024-04-10T21:10:21Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dairy Science |
| spelling | doaj.art-db910f5be01a45b19212c75abea041962023-01-21T04:19:51ZengElsevierJournal of Dairy Science0022-03022023-02-01106214141428Effects of additional gonadotropin-releasing hormone and prostaglandin F2α treatment to an estradiol/progesterone-based embryo transfer protocol for recipient lactating dairy cowsM.H.C. Pereira0B.I. Cappellozza1R.L.A. Cerri2C.P. Sanches, Jr.3T.G. Guida4L.F.S.P. Barbosa5J.E.P. Santos6J.L.M. Vasconcelos7Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista (UNESP), Botucatu, SP 18618-000, BrazilChr. Hansen Animal Health & Nutrition, Hoersholm, 2970, DenmarkFaculty of Land and Food Systems, University of British Columbia, Vancouver, Canada V6T 1Z4Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista (UNESP), Botucatu, SP 18618-000, BrazilFaculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista (UNESP), Botucatu, SP 18618-000, BrazilFaculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista (UNESP), Botucatu, SP 18618-000, BrazilDepartment of Animal Sciences, D.H. Barron Reproductive and Perinatal Biology Research Program, University of Florida, Gainesville 32611Department of Animal Production, São Paulo State University, Botucatu 18168-000, Brazil; Corresponding authorABSTRACT: This study was designed to evaluate whether the utilization of a second PGF2α treatment at the end of an estradiol/progesterone (E2/P4)-based protocol with or without GnRH at the beginning of the protocol would improve pregnancy rates of lactating Holstein cows assigned to timed embryo transfer. A total of 501 lactating Holstein cows in 5 farms were enrolled in the experiment. Within farm, cows were blocked by parity and, within block, were assigned randomly to (1) insertion of an intravaginal P4 device (controlled internal drug-releasing device; CIDR) and estradiol benzoate on d −11, PGF2α on d −4, CIDR withdrawal and an injection of estradiol cypionate on d −2, and timed embryo transfer on d 7 (1-PGF; n = 164); (2) the same treatments as 1-PGF, but with PGF2α administered on d −4 and −2 (2-PGF; n = 171); and (3) 2-PGF with the addition of a GnRH treatment on d −11 (GnRH+2-PGF; n = 166). Ovaries were scanned by transrectal ultrasonography on d −11, −4, and 7, and blood samples were collected on d −11, −4, 0, and 7 for P4 determination. Treatment comparisons were performed using contrasts. The proportion of cows with a new corpus luteum on d −4 was greater in GnRH+2-PGF cows. Cows in 1-PGF had a greater P4 concentration on d 0 but lesser P4 on d 7 compared with cows in the other groups. Cows assigned to receive 2-PGF (2-PGF and GnRH+2-PGF) had greater estrus expression, and a greater proportion of cows ovulated to estradiol cypionate. No further contrast effects were observed for follicle diameter, double ovulation rate, pregnancy per embryo transfer (P/ET) on d 32 and 60, or pregnancy loss. As P4 concentration on d −4 increased, P/ET on d 60 tended to increase. Cows with P4 ≥3.66 ng/mL on d −4 had greater P/ET on d 32 and 60 than those with P4 below that threshold. Regardless of treatment, cows with P4 concentration ≥3.66 ng/mL also had a greater pregnancy per synchronized protocol (P/SP) on d 60. Also, a P4 concentration on d −4 (low or high) × follicle diameter (continuous) interaction tendency was observed when evaluating P/ET. Although P/ET did not differ among cows with different follicles sizes with reduced P4 concentration on d −4 (<3.66 ng/mL), it increased in cows with larger follicles exposed to increased P4 concentration (≥3.66 ng/mL). When P4 on d 0 was evaluated, P/ET on d 32 and 60 was greater for cows with low (≤0.09 ng/mL) versus high (>0.21 ng/mL) P4; as P4 concentration on d 0 increased, P/ET linearly decreased. In summary, cows with increased P4 concentrations during growth of the ovulatory follicular wave had improved P/ET. Administering a second PGF2α dose reduced P4 concentration on d 0 and increased ovulatory response to the protocol, but no benefits were observed on P/ET or P/SP.http://www.sciencedirect.com/science/article/pii/S0022030222006956embryo transfergonadotropin-releasing hormoneprogesteroneprostaglandin |
| spellingShingle | M.H.C. Pereira B.I. Cappellozza R.L.A. Cerri C.P. Sanches, Jr. T.G. Guida L.F.S.P. Barbosa J.E.P. Santos J.L.M. Vasconcelos Effects of additional gonadotropin-releasing hormone and prostaglandin F2α treatment to an estradiol/progesterone-based embryo transfer protocol for recipient lactating dairy cows Journal of Dairy Science embryo transfer gonadotropin-releasing hormone progesterone prostaglandin |
| title | Effects of additional gonadotropin-releasing hormone and prostaglandin F2α treatment to an estradiol/progesterone-based embryo transfer protocol for recipient lactating dairy cows |
| title_full | Effects of additional gonadotropin-releasing hormone and prostaglandin F2α treatment to an estradiol/progesterone-based embryo transfer protocol for recipient lactating dairy cows |
| title_fullStr | Effects of additional gonadotropin-releasing hormone and prostaglandin F2α treatment to an estradiol/progesterone-based embryo transfer protocol for recipient lactating dairy cows |
| title_full_unstemmed | Effects of additional gonadotropin-releasing hormone and prostaglandin F2α treatment to an estradiol/progesterone-based embryo transfer protocol for recipient lactating dairy cows |
| title_short | Effects of additional gonadotropin-releasing hormone and prostaglandin F2α treatment to an estradiol/progesterone-based embryo transfer protocol for recipient lactating dairy cows |
| title_sort | effects of additional gonadotropin releasing hormone and prostaglandin f2α treatment to an estradiol progesterone based embryo transfer protocol for recipient lactating dairy cows |
| topic | embryo transfer gonadotropin-releasing hormone progesterone prostaglandin |
| url | http://www.sciencedirect.com/science/article/pii/S0022030222006956 |
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