The Degradation of Intramuscular Connective Tissue In Vitro with Purified Cathepsin L from Bovine Pancreas
To investigate the possible degradation of the intramuscular connective tissue (IMCT) with cathepsin L, isolated IMCTs were incubated with purified cathepsin L in vitro. Here, we prepared purified cathepsin L from bovine pancreas by using DEAE Sephacel, Sephacryl S-100 HR, SP Sepharose FF, and con A...
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MDPI AG
2023-09-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/18/3517 |
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| author | Yingbo Peng Wanhong He Shuang Teng Muneer Ahmed Jamali |
| author_facet | Yingbo Peng Wanhong He Shuang Teng Muneer Ahmed Jamali |
| author_sort | Yingbo Peng |
| collection | DOAJ |
| description | To investigate the possible degradation of the intramuscular connective tissue (IMCT) with cathepsin L, isolated IMCTs were incubated with purified cathepsin L in vitro. Here, we prepared purified cathepsin L from bovine pancreas by using DEAE Sephacel, Sephacryl S-100 HR, SP Sepharose FF, and con A-Sepharose affinity chromatography in sequence. An SDS-PAGE analysis of CNBr-digested peptides showed that the degradation of collagen in IMCT could take place on terminal non-helical peptides rather than the triple helix region. Decorin (DCN) was clearly degraded at a pH of 5.0. The T<sub>P</sub> and T<sub>O</sub> of intramuscular connective tissue decreased to 41.41 °C and 43.79 °C, respectively. In the cathepsin L treatment of pH 5.0, the decreases in the T<sub>P</sub> and T<sub>O</sub> of IMCT were more sensitive than they were at pH 5.5~6.5. |
| first_indexed | 2024-03-10T22:45:46Z |
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| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T22:45:46Z |
| publishDate | 2023-09-01 |
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| spelling | doaj.art-db9383e84fb447cf94661a8f94d915e02023-11-19T10:44:18ZengMDPI AGFoods2304-81582023-09-011218351710.3390/foods12183517The Degradation of Intramuscular Connective Tissue In Vitro with Purified Cathepsin L from Bovine PancreasYingbo Peng0Wanhong He1Shuang Teng2Muneer Ahmed Jamali3College of Engineering, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Animal Products Technology, Sindh Agriculture University, Tandojam 70060, PakistanTo investigate the possible degradation of the intramuscular connective tissue (IMCT) with cathepsin L, isolated IMCTs were incubated with purified cathepsin L in vitro. Here, we prepared purified cathepsin L from bovine pancreas by using DEAE Sephacel, Sephacryl S-100 HR, SP Sepharose FF, and con A-Sepharose affinity chromatography in sequence. An SDS-PAGE analysis of CNBr-digested peptides showed that the degradation of collagen in IMCT could take place on terminal non-helical peptides rather than the triple helix region. Decorin (DCN) was clearly degraded at a pH of 5.0. The T<sub>P</sub> and T<sub>O</sub> of intramuscular connective tissue decreased to 41.41 °C and 43.79 °C, respectively. In the cathepsin L treatment of pH 5.0, the decreases in the T<sub>P</sub> and T<sub>O</sub> of IMCT were more sensitive than they were at pH 5.5~6.5.https://www.mdpi.com/2304-8158/12/18/3517intramuscular connective tissuecathepsin Lcollagendecorindegradation |
| spellingShingle | Yingbo Peng Wanhong He Shuang Teng Muneer Ahmed Jamali The Degradation of Intramuscular Connective Tissue In Vitro with Purified Cathepsin L from Bovine Pancreas Foods intramuscular connective tissue cathepsin L collagen decorin degradation |
| title | The Degradation of Intramuscular Connective Tissue In Vitro with Purified Cathepsin L from Bovine Pancreas |
| title_full | The Degradation of Intramuscular Connective Tissue In Vitro with Purified Cathepsin L from Bovine Pancreas |
| title_fullStr | The Degradation of Intramuscular Connective Tissue In Vitro with Purified Cathepsin L from Bovine Pancreas |
| title_full_unstemmed | The Degradation of Intramuscular Connective Tissue In Vitro with Purified Cathepsin L from Bovine Pancreas |
| title_short | The Degradation of Intramuscular Connective Tissue In Vitro with Purified Cathepsin L from Bovine Pancreas |
| title_sort | degradation of intramuscular connective tissue in vitro with purified cathepsin l from bovine pancreas |
| topic | intramuscular connective tissue cathepsin L collagen decorin degradation |
| url | https://www.mdpi.com/2304-8158/12/18/3517 |
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