Dynamic of Composition and Diversity of Gut Microbiota in Triatoma rubrofasciata in Different Developmental Stages and Environmental Conditions
Triatoma rubrofasciata (T. rubrofasciata), one kind of triatomine insects, is the vector of Trypanosoma cruzi (T. cruzi), which lead to American trypanosomiasis. Although the gut microbiome may play an essential role in the development and susceptibility of triatomine, there is limited research on t...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-11-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2020.587708/full |
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| author | Yue Hu Yue Hu Yue Hu Hanguo Xie Minzhao Gao Ping Huang Ping Huang Ping Huang Hongli Zhou Hongli Zhou Yubin Ma Yubin Ma Minyu Zhou Minyu Zhou Jinying Liang Jinying Liang Jun Yang Zhiyue Lv Zhiyue Lv Zhiyue Lv |
| author_facet | Yue Hu Yue Hu Yue Hu Hanguo Xie Minzhao Gao Ping Huang Ping Huang Ping Huang Hongli Zhou Hongli Zhou Yubin Ma Yubin Ma Minyu Zhou Minyu Zhou Jinying Liang Jinying Liang Jun Yang Zhiyue Lv Zhiyue Lv Zhiyue Lv |
| author_sort | Yue Hu |
| collection | DOAJ |
| description | Triatoma rubrofasciata (T. rubrofasciata), one kind of triatomine insects, is the vector of Trypanosoma cruzi (T. cruzi), which lead to American trypanosomiasis. Although the gut microbiome may play an essential role in the development and susceptibility of triatomine, there is limited research on the gut microbiota of T. rubrofasciata. To elucidate the effect of the vector’s developmental stages and environmental conditions on the gut microbiome, we employed 16S rRNA gene sequencing to profile the gut bacterial community diversity and composition of T. rubrofasciata. Significant shifts were observed in the overall gut microbe diversity and composition across the development of T. rubrofasciata and specific bacteria were detected in different stages. Serratia and Burkholderia-Caballeronia-Paraburkholderia were dominant in the 1st nymphal stage, while the abundance of Staphylococcus was low in the 1st nymphal stage. Oceanicaulis were undetectable in the adult stage and Odoribacter peaked in the 2nd nymphal stage. Moreover, Staphylococcus was correlated negatively with Serratia. Likewise, the total gut microbiota diversity and composition of T. rubrofasciata differentiated significantly by environmental conditions. The ingestion of a bloodmeal increased alpha diversity of gut bacterial communities, and Staphylococcus was more abundant in laboratory-reared bugs whereas Enterococcus enriched in wild-caught bugs. Furthermore, Pantoea was negatively correlated with Staphylococcus, and positively related to Bacillus only. The phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) algorithm showed obvious metagenomic functional differences by environmental conditions, and Chagas disease relevant pathway was enriched in wild-caught T. rubrofasciata. |
| first_indexed | 2024-12-13T05:22:22Z |
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| id | doaj.art-db93f0b5a2a740718b6aef17825e77f2 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-13T05:22:22Z |
| publishDate | 2020-11-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-db93f0b5a2a740718b6aef17825e77f22022-12-21T23:58:18ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882020-11-011010.3389/fcimb.2020.587708587708Dynamic of Composition and Diversity of Gut Microbiota in Triatoma rubrofasciata in Different Developmental Stages and Environmental ConditionsYue Hu0Yue Hu1Yue Hu2Hanguo Xie3Minzhao Gao4Ping Huang5Ping Huang6Ping Huang7Hongli Zhou8Hongli Zhou9Yubin Ma10Yubin Ma11Minyu Zhou12Minyu Zhou13Jinying Liang14Jinying Liang15Jun Yang16Zhiyue Lv17Zhiyue Lv18Zhiyue Lv19Joint Program of Pathobiology, Fifth Affiliated Hospital, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, ChinaKey Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, ChinaKey Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of Education, Guangzhou, ChinaProvincial Key Laboratory of Zoonosis Research, Fujian Center for Disease Control and Prevention, Fuzhou, ChinaJoint Program of Pathobiology, Fifth Affiliated Hospital, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, ChinaJoint Program of Pathobiology, Fifth Affiliated Hospital, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, ChinaKey Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, ChinaKey Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of Education, Guangzhou, ChinaJoint Program of Pathobiology, Fifth Affiliated Hospital, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, ChinaKey Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of Education, Guangzhou, ChinaJoint Program of Pathobiology, Fifth Affiliated Hospital, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, ChinaKey Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of Education, Guangzhou, ChinaJoint Program of Pathobiology, Fifth Affiliated Hospital, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, ChinaKey Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of Education, Guangzhou, ChinaJoint Program of Pathobiology, Fifth Affiliated Hospital, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, ChinaKey Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of Education, Guangzhou, ChinaKey Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, ChinaJoint Program of Pathobiology, Fifth Affiliated Hospital, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, ChinaKey Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, ChinaKey Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of Education, Guangzhou, ChinaTriatoma rubrofasciata (T. rubrofasciata), one kind of triatomine insects, is the vector of Trypanosoma cruzi (T. cruzi), which lead to American trypanosomiasis. Although the gut microbiome may play an essential role in the development and susceptibility of triatomine, there is limited research on the gut microbiota of T. rubrofasciata. To elucidate the effect of the vector’s developmental stages and environmental conditions on the gut microbiome, we employed 16S rRNA gene sequencing to profile the gut bacterial community diversity and composition of T. rubrofasciata. Significant shifts were observed in the overall gut microbe diversity and composition across the development of T. rubrofasciata and specific bacteria were detected in different stages. Serratia and Burkholderia-Caballeronia-Paraburkholderia were dominant in the 1st nymphal stage, while the abundance of Staphylococcus was low in the 1st nymphal stage. Oceanicaulis were undetectable in the adult stage and Odoribacter peaked in the 2nd nymphal stage. Moreover, Staphylococcus was correlated negatively with Serratia. Likewise, the total gut microbiota diversity and composition of T. rubrofasciata differentiated significantly by environmental conditions. The ingestion of a bloodmeal increased alpha diversity of gut bacterial communities, and Staphylococcus was more abundant in laboratory-reared bugs whereas Enterococcus enriched in wild-caught bugs. Furthermore, Pantoea was negatively correlated with Staphylococcus, and positively related to Bacillus only. The phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) algorithm showed obvious metagenomic functional differences by environmental conditions, and Chagas disease relevant pathway was enriched in wild-caught T. rubrofasciata.https://www.frontiersin.org/articles/10.3389/fcimb.2020.587708/fullTriatoma rubrofasciatagut microbiotadevelopmental stagesenvironmental conditions16S rRNA gene sequencing |
| spellingShingle | Yue Hu Yue Hu Yue Hu Hanguo Xie Minzhao Gao Ping Huang Ping Huang Ping Huang Hongli Zhou Hongli Zhou Yubin Ma Yubin Ma Minyu Zhou Minyu Zhou Jinying Liang Jinying Liang Jun Yang Zhiyue Lv Zhiyue Lv Zhiyue Lv Dynamic of Composition and Diversity of Gut Microbiota in Triatoma rubrofasciata in Different Developmental Stages and Environmental Conditions Frontiers in Cellular and Infection Microbiology Triatoma rubrofasciata gut microbiota developmental stages environmental conditions 16S rRNA gene sequencing |
| title | Dynamic of Composition and Diversity of Gut Microbiota in Triatoma rubrofasciata in Different Developmental Stages and Environmental Conditions |
| title_full | Dynamic of Composition and Diversity of Gut Microbiota in Triatoma rubrofasciata in Different Developmental Stages and Environmental Conditions |
| title_fullStr | Dynamic of Composition and Diversity of Gut Microbiota in Triatoma rubrofasciata in Different Developmental Stages and Environmental Conditions |
| title_full_unstemmed | Dynamic of Composition and Diversity of Gut Microbiota in Triatoma rubrofasciata in Different Developmental Stages and Environmental Conditions |
| title_short | Dynamic of Composition and Diversity of Gut Microbiota in Triatoma rubrofasciata in Different Developmental Stages and Environmental Conditions |
| title_sort | dynamic of composition and diversity of gut microbiota in triatoma rubrofasciata in different developmental stages and environmental conditions |
| topic | Triatoma rubrofasciata gut microbiota developmental stages environmental conditions 16S rRNA gene sequencing |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2020.587708/full |
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