AaABCG40 Enhances Artemisinin Content and Modulates Drought Tolerance in Artemisia annua
The phytohormone Abscisic acid (ABA) regulates plant growth, development, and responses to abiotic stresses, including senescence, seed germination, cold stress and drought. Several kinds of researches indicate that exogenous ABA can enhance artemisinin content in A. annua. Some transcription factor...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-06-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2020.00950/full |
| _version_ | 1818255727956852736 |
|---|---|
| author | Xueqing Fu Hang Liu Danial Hassani Bowen Peng Xin Yan Yuting Wang Chen Wang Ling Li Pin Liu Qifang Pan Jingya Zhao Hongmei Qian Xiaofen Sun Kexuan Tang |
| author_facet | Xueqing Fu Hang Liu Danial Hassani Bowen Peng Xin Yan Yuting Wang Chen Wang Ling Li Pin Liu Qifang Pan Jingya Zhao Hongmei Qian Xiaofen Sun Kexuan Tang |
| author_sort | Xueqing Fu |
| collection | DOAJ |
| description | The phytohormone Abscisic acid (ABA) regulates plant growth, development, and responses to abiotic stresses, including senescence, seed germination, cold stress and drought. Several kinds of researches indicate that exogenous ABA can enhance artemisinin content in A. annua. Some transcription factors related to ABA signaling are identified to increase artemisinin accumulation through activating the artemisinin synthase genes. However, no prior study on ABA transporter has been performed in A. annua. Here, we identified a pleiotropic drug resistance (PDR) transporter gene AaPDR4/AaABCG40 from A. annua. AaABCG40 was expressed mainly in roots, leaves, buds, and trichomes. GUS activity is primarily observed in roots and the vascular tissues of young leaves in proAaABCG40: GUS transgenic A. annua plants. When AaABCG40 was transferred into yeast AD12345678, yeasts expressing AaABCG40 accumulated more ABA than the control. The AaABCG40 overexpressing plants showed higher artemisinin content and stronger drought tolerance. Besides, the expression of CYP71AV1 in OE-AaABCG40 plants showed more sensitivity to exogenous ABA than that in both wild-type and iAaABCG40 plants. According to these results, they strongly suggest that AaABCG40 is involved in ABA transport in A. annua. |
| first_indexed | 2024-12-12T17:16:28Z |
| format | Article |
| id | doaj.art-db95dc6425444c398df60d97a42d5598 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-12T17:16:28Z |
| publishDate | 2020-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-db95dc6425444c398df60d97a42d55982022-12-22T00:17:46ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2020-06-011110.3389/fpls.2020.00950524733AaABCG40 Enhances Artemisinin Content and Modulates Drought Tolerance in Artemisia annuaXueqing FuHang LiuDanial HassaniBowen PengXin YanYuting WangChen WangLing LiPin LiuQifang PanJingya ZhaoHongmei QianXiaofen SunKexuan TangThe phytohormone Abscisic acid (ABA) regulates plant growth, development, and responses to abiotic stresses, including senescence, seed germination, cold stress and drought. Several kinds of researches indicate that exogenous ABA can enhance artemisinin content in A. annua. Some transcription factors related to ABA signaling are identified to increase artemisinin accumulation through activating the artemisinin synthase genes. However, no prior study on ABA transporter has been performed in A. annua. Here, we identified a pleiotropic drug resistance (PDR) transporter gene AaPDR4/AaABCG40 from A. annua. AaABCG40 was expressed mainly in roots, leaves, buds, and trichomes. GUS activity is primarily observed in roots and the vascular tissues of young leaves in proAaABCG40: GUS transgenic A. annua plants. When AaABCG40 was transferred into yeast AD12345678, yeasts expressing AaABCG40 accumulated more ABA than the control. The AaABCG40 overexpressing plants showed higher artemisinin content and stronger drought tolerance. Besides, the expression of CYP71AV1 in OE-AaABCG40 plants showed more sensitivity to exogenous ABA than that in both wild-type and iAaABCG40 plants. According to these results, they strongly suggest that AaABCG40 is involved in ABA transport in A. annua.https://www.frontiersin.org/article/10.3389/fpls.2020.00950/fullArtemisia annuaartemisininpleiotropic drug resistance (PDR) transporterdrought toleranceabscisic acid |
| spellingShingle | Xueqing Fu Hang Liu Danial Hassani Bowen Peng Xin Yan Yuting Wang Chen Wang Ling Li Pin Liu Qifang Pan Jingya Zhao Hongmei Qian Xiaofen Sun Kexuan Tang AaABCG40 Enhances Artemisinin Content and Modulates Drought Tolerance in Artemisia annua Frontiers in Plant Science Artemisia annua artemisinin pleiotropic drug resistance (PDR) transporter drought tolerance abscisic acid |
| title | AaABCG40 Enhances Artemisinin Content and Modulates Drought Tolerance in Artemisia annua |
| title_full | AaABCG40 Enhances Artemisinin Content and Modulates Drought Tolerance in Artemisia annua |
| title_fullStr | AaABCG40 Enhances Artemisinin Content and Modulates Drought Tolerance in Artemisia annua |
| title_full_unstemmed | AaABCG40 Enhances Artemisinin Content and Modulates Drought Tolerance in Artemisia annua |
| title_short | AaABCG40 Enhances Artemisinin Content and Modulates Drought Tolerance in Artemisia annua |
| title_sort | aaabcg40 enhances artemisinin content and modulates drought tolerance in artemisia annua |
| topic | Artemisia annua artemisinin pleiotropic drug resistance (PDR) transporter drought tolerance abscisic acid |
| url | https://www.frontiersin.org/article/10.3389/fpls.2020.00950/full |
| work_keys_str_mv | AT xueqingfu aaabcg40enhancesartemisinincontentandmodulatesdroughttoleranceinartemisiaannua AT hangliu aaabcg40enhancesartemisinincontentandmodulatesdroughttoleranceinartemisiaannua AT danialhassani aaabcg40enhancesartemisinincontentandmodulatesdroughttoleranceinartemisiaannua AT bowenpeng aaabcg40enhancesartemisinincontentandmodulatesdroughttoleranceinartemisiaannua AT xinyan aaabcg40enhancesartemisinincontentandmodulatesdroughttoleranceinartemisiaannua AT yutingwang aaabcg40enhancesartemisinincontentandmodulatesdroughttoleranceinartemisiaannua AT chenwang aaabcg40enhancesartemisinincontentandmodulatesdroughttoleranceinartemisiaannua AT lingli aaabcg40enhancesartemisinincontentandmodulatesdroughttoleranceinartemisiaannua AT pinliu aaabcg40enhancesartemisinincontentandmodulatesdroughttoleranceinartemisiaannua AT qifangpan aaabcg40enhancesartemisinincontentandmodulatesdroughttoleranceinartemisiaannua AT jingyazhao aaabcg40enhancesartemisinincontentandmodulatesdroughttoleranceinartemisiaannua AT hongmeiqian aaabcg40enhancesartemisinincontentandmodulatesdroughttoleranceinartemisiaannua AT xiaofensun aaabcg40enhancesartemisinincontentandmodulatesdroughttoleranceinartemisiaannua AT kexuantang aaabcg40enhancesartemisinincontentandmodulatesdroughttoleranceinartemisiaannua |