Scalable continuous evolution for the generation of diverse enzyme variants encompassing promiscuous activities

Generating rich ortholog diversity for biocatalysts can be difficult due to the deep evolutionary processes involved. Here the authors use OrthoRep to rapidly evolve TrpB to produce sequence-diverse variants with altered substrate promiscuity.

Bibliographic Details
Main Authors: Gordon Rix, Ella J. Watkins-Dulaney, Patrick J. Almhjell, Christina E. Boville, Frances H. Arnold, Chang C. Liu
Format: Article
Language:English
Published: Nature Portfolio 2020-11-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-19539-6

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