Detection of carbapenemase production in Enterobacteriaceae and Pseudomonas species by carbapenemase Nordmann–Poirel test
PURPOSE: Multidrug-resistant organisms causing community-acquired and hospital-acquired infections are increasing at a dangerous rate. Carbapenemase-producing Enterobacteriaceae and Pseudomonas species are an important source of concern since these organisms are not only resistant to beta-lactam ant...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Thieme Medical and Scientific Publishers Pvt. Ltd.
2019-04-01
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| Series: | Journal of Laboratory Physicians |
| Subjects: | |
| Online Access: | http://www.thieme-connect.de/DOI/DOI?10.4103/JLP.JLP_132_18 |
| _version_ | 1819011410792808448 |
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| author | Morubagal R. Rao Pooja Chandrashaker Rashmi P. Mahale Sowmya G. Shivappa Ranjitha S. Gowda Vidyavathi B. Chitharagi |
| author_facet | Morubagal R. Rao Pooja Chandrashaker Rashmi P. Mahale Sowmya G. Shivappa Ranjitha S. Gowda Vidyavathi B. Chitharagi |
| author_sort | Morubagal R. Rao |
| collection | DOAJ |
| description | PURPOSE: Multidrug-resistant organisms causing community-acquired and hospital-acquired infections are increasing at a dangerous rate. Carbapenemase-producing Enterobacteriaceae and Pseudomonas species are an important source of concern since these organisms are not only resistant to beta-lactam antibiotics but also show cross-resistance to other groups of antibiotics. In the present study, rapid detection of these carbapenemase-producing Enterobacteriaceae and Pseudomonas species by carbapenemase Nordmann–Poirel (Carba NP) test was evaluated by comparing with modified Hodge test (MHT).
MATERIALS AND METHODS: Imipenem-resistant Enterobacteriaceae and Pseudomonas species isolated from various samples such as pus, blood, sputum, urine, and endotracheal aspirates were processed for carbapenemase detection by MHT and Carba NP test. Kappa analysis was done to evaluate the percentage agreement between the two tests.
RESULTS: Seventy imipenem-resistant Enterobacteriaceae and Pseudomonas isolates were analyzed in the present study for carbapenemase production. 63.41% ofEnterobacteriaceae and 34.48% of Pseudomonas species were carbapenemase producers considering both the methods. By MHT, 36 (51.42%) isolates and, by Carba NP test, 35 (50%) isolates were positive for carbapenemase production out of the 70 isolates.
CONCLUSION: Carba NP test when compared to MHT is a simple, rapid, cost-effective biochemical test which can be used in all laboratories in the identification of life-threatening carbapenemase-producing Gram-negative bacteria. |
| first_indexed | 2024-12-21T01:27:43Z |
| format | Article |
| id | doaj.art-db9ff52a7f274ad6bf95197906423752 |
| institution | Directory Open Access Journal |
| issn | 0974-2727 0974-7826 |
| language | English |
| last_indexed | 2024-12-21T01:27:43Z |
| publishDate | 2019-04-01 |
| publisher | Thieme Medical and Scientific Publishers Pvt. Ltd. |
| record_format | Article |
| series | Journal of Laboratory Physicians |
| spelling | doaj.art-db9ff52a7f274ad6bf951979064237522022-12-21T19:20:28ZengThieme Medical and Scientific Publishers Pvt. Ltd.Journal of Laboratory Physicians0974-27270974-78262019-04-01110210711010.4103/JLP.JLP_132_18Detection of carbapenemase production in Enterobacteriaceae and Pseudomonas species by carbapenemase Nordmann–Poirel testMorubagal R. Rao0Pooja Chandrashaker1Rashmi P. Mahale2Sowmya G. Shivappa3Ranjitha S. Gowda4Vidyavathi B. Chitharagi5Department of Microbiology, JSS Medical College, JSS Academy of Higher Education and Research, Mysore, Karnataka, IndiaDepartment of Microbiology, JSS Medical College, JSS Academy of Higher Education and Research, Mysore, Karnataka, IndiaDepartment of Microbiology, JSS Medical College, JSS Academy of Higher Education and Research, Mysore, Karnataka, IndiaDepartment of Microbiology, JSS Medical College, JSS Academy of Higher Education and Research, Mysore, Karnataka, IndiaDepartment of Microbiology, JSS Medical College, JSS Academy of Higher Education and Research, Mysore, Karnataka, IndiaDepartment of Microbiology, JSS Medical College, JSS Academy of Higher Education and Research, Mysore, Karnataka, IndiaPURPOSE: Multidrug-resistant organisms causing community-acquired and hospital-acquired infections are increasing at a dangerous rate. Carbapenemase-producing Enterobacteriaceae and Pseudomonas species are an important source of concern since these organisms are not only resistant to beta-lactam antibiotics but also show cross-resistance to other groups of antibiotics. In the present study, rapid detection of these carbapenemase-producing Enterobacteriaceae and Pseudomonas species by carbapenemase Nordmann–Poirel (Carba NP) test was evaluated by comparing with modified Hodge test (MHT). MATERIALS AND METHODS: Imipenem-resistant Enterobacteriaceae and Pseudomonas species isolated from various samples such as pus, blood, sputum, urine, and endotracheal aspirates were processed for carbapenemase detection by MHT and Carba NP test. Kappa analysis was done to evaluate the percentage agreement between the two tests. RESULTS: Seventy imipenem-resistant Enterobacteriaceae and Pseudomonas isolates were analyzed in the present study for carbapenemase production. 63.41% ofEnterobacteriaceae and 34.48% of Pseudomonas species were carbapenemase producers considering both the methods. By MHT, 36 (51.42%) isolates and, by Carba NP test, 35 (50%) isolates were positive for carbapenemase production out of the 70 isolates. CONCLUSION: Carba NP test when compared to MHT is a simple, rapid, cost-effective biochemical test which can be used in all laboratories in the identification of life-threatening carbapenemase-producing Gram-negative bacteria.http://www.thieme-connect.de/DOI/DOI?10.4103/JLP.JLP_132_18carbapenemasecarbapenemase nordmann–poirelenterobacteriaceaemodified hodge testpseudomonas |
| spellingShingle | Morubagal R. Rao Pooja Chandrashaker Rashmi P. Mahale Sowmya G. Shivappa Ranjitha S. Gowda Vidyavathi B. Chitharagi Detection of carbapenemase production in Enterobacteriaceae and Pseudomonas species by carbapenemase Nordmann–Poirel test Journal of Laboratory Physicians carbapenemase carbapenemase nordmann–poirel enterobacteriaceae modified hodge test pseudomonas |
| title | Detection of carbapenemase production in Enterobacteriaceae and Pseudomonas species by carbapenemase Nordmann–Poirel test |
| title_full | Detection of carbapenemase production in Enterobacteriaceae and Pseudomonas species by carbapenemase Nordmann–Poirel test |
| title_fullStr | Detection of carbapenemase production in Enterobacteriaceae and Pseudomonas species by carbapenemase Nordmann–Poirel test |
| title_full_unstemmed | Detection of carbapenemase production in Enterobacteriaceae and Pseudomonas species by carbapenemase Nordmann–Poirel test |
| title_short | Detection of carbapenemase production in Enterobacteriaceae and Pseudomonas species by carbapenemase Nordmann–Poirel test |
| title_sort | detection of carbapenemase production in enterobacteriaceae and pseudomonas species by carbapenemase nordmann poirel test |
| topic | carbapenemase carbapenemase nordmann–poirel enterobacteriaceae modified hodge test pseudomonas |
| url | http://www.thieme-connect.de/DOI/DOI?10.4103/JLP.JLP_132_18 |
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