Genetic diversity, chemical constituents and anatomical analysis of eight popular Olive (Olea europaea L.) cultivars in Al-Jouf region, Saudi Arabia
Abstract In light of the multitude of olive trees cultivated and the lack of the genetic diversity of available genotypes to select varieties and lines that are characterized by high diversity and better performance under the corresponding conditions, A comparison analysis of the genotyping and morp...
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| Format: | Article |
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Nature Portfolio
2024-06-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-65542-y |
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| author | Haifa A. S. Alhaithloul Nabil S. Awad Sameer H. Qari Rania F. El-Homosy El-Sayed M. Qaoud Mesfer M. Alqahtani Kholoud Z. Ghanem Abdulrahman Alasmari Fahad M. Alzuaibr Hesham S. Ghazzawy Mohamed A. Abdein |
| author_facet | Haifa A. S. Alhaithloul Nabil S. Awad Sameer H. Qari Rania F. El-Homosy El-Sayed M. Qaoud Mesfer M. Alqahtani Kholoud Z. Ghanem Abdulrahman Alasmari Fahad M. Alzuaibr Hesham S. Ghazzawy Mohamed A. Abdein |
| author_sort | Haifa A. S. Alhaithloul |
| collection | DOAJ |
| description | Abstract In light of the multitude of olive trees cultivated and the lack of the genetic diversity of available genotypes to select varieties and lines that are characterized by high diversity and better performance under the corresponding conditions, A comparison analysis of the genotyping and morphological characteristics of eight olive cultivars growing in Saudi Arabia’s Al-Jouf region was conducted and analyzed. Morpho-anatomical and chemical characteristics along with both inter-simple-sequence repeats (ISSRs) and start-codon-targeted (SCoT) markers were used to evaluate the genetic diversity among eight olive varieties in Al-Jouf, Saudi Arabia. Analyses of 27 morphological, chemical, and anatomical characteristics concluded the existence of genetic differences among the studied varieties. Moreover, six ISSR and eight SCoT primer combinations produced a total of 48 loci, of which 18 (10 ISSR and 8 SCoT) were polymorphic. The average polymorphism information content (PIC values of 0.48 and 0.44, respectively) and marker index (MI of 0.79 and 0.48, respectively) detected for ISSR and SCoT markers revealed the prevalence of high genetic diversity among the studied olive varieties. Based on chemical and anatomical characteristics and the selected molecular markers, the eight olive cultivars were grouped into two distinct clusters. Clusters in the adjacent joint dendrogram produced using ISSR, SCoT and combined data were similar, and grouped all individuals into two groups. However, the dendrogram generated on the basis of SCoT separated individuals into subgroups containing at least two varieties. The findings showed that both methods were effective in assessing diversity, and that SCoT markers can be used as a reliable and informative method for assessing genetic diversity and relationships among olive varieties and can serve as a complementary tool to provide a more complete understanding of the genetic diversity available in Olea europaea populations in Saudi Arabia. |
| first_indexed | 2025-03-21T12:07:25Z |
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| id | doaj.art-dba21af2a8d14b4ab1f1c38d66d23f16 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2025-03-21T12:07:25Z |
| publishDate | 2024-06-01 |
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| spelling | doaj.art-dba21af2a8d14b4ab1f1c38d66d23f162024-06-30T11:19:04ZengNature PortfolioScientific Reports2045-23222024-06-0114111710.1038/s41598-024-65542-yGenetic diversity, chemical constituents and anatomical analysis of eight popular Olive (Olea europaea L.) cultivars in Al-Jouf region, Saudi ArabiaHaifa A. S. Alhaithloul0Nabil S. Awad1Sameer H. Qari2Rania F. El-Homosy3El-Sayed M. Qaoud4Mesfer M. Alqahtani5Kholoud Z. Ghanem6Abdulrahman Alasmari7Fahad M. Alzuaibr8Hesham S. Ghazzawy9Mohamed A. Abdein10Biology Department, College of Science, Jouf UniversityDepartment of Genetics, Faculty of Agriculture and Natural Resources, Aswan UniversityBiology Department, Genetics and Molecular Biology Central Laboratory, Aljumum University College, Umm Al-Qura UniversityGenetics Department, Faculty of Agriculture, Assiut UniversityHorticultural Department, Faculty of Agriculture, Suez Canal UniversityDepartment of Biological Sciences, Faculty of Science and Humanities, Shaqra UniversityDepartment of Biological Science, College of Science &Humanities, Shaqra UniversityBiology Department, College of Science, University of TabukBiology Department, College of Science, University of TabukDate Palm Research Center of Excellence, King Faisal UniversitySeeds Development Department, El-Nada Misr Scientific Research and Development ProjectsAbstract In light of the multitude of olive trees cultivated and the lack of the genetic diversity of available genotypes to select varieties and lines that are characterized by high diversity and better performance under the corresponding conditions, A comparison analysis of the genotyping and morphological characteristics of eight olive cultivars growing in Saudi Arabia’s Al-Jouf region was conducted and analyzed. Morpho-anatomical and chemical characteristics along with both inter-simple-sequence repeats (ISSRs) and start-codon-targeted (SCoT) markers were used to evaluate the genetic diversity among eight olive varieties in Al-Jouf, Saudi Arabia. Analyses of 27 morphological, chemical, and anatomical characteristics concluded the existence of genetic differences among the studied varieties. Moreover, six ISSR and eight SCoT primer combinations produced a total of 48 loci, of which 18 (10 ISSR and 8 SCoT) were polymorphic. The average polymorphism information content (PIC values of 0.48 and 0.44, respectively) and marker index (MI of 0.79 and 0.48, respectively) detected for ISSR and SCoT markers revealed the prevalence of high genetic diversity among the studied olive varieties. Based on chemical and anatomical characteristics and the selected molecular markers, the eight olive cultivars were grouped into two distinct clusters. Clusters in the adjacent joint dendrogram produced using ISSR, SCoT and combined data were similar, and grouped all individuals into two groups. However, the dendrogram generated on the basis of SCoT separated individuals into subgroups containing at least two varieties. The findings showed that both methods were effective in assessing diversity, and that SCoT markers can be used as a reliable and informative method for assessing genetic diversity and relationships among olive varieties and can serve as a complementary tool to provide a more complete understanding of the genetic diversity available in Olea europaea populations in Saudi Arabia.https://doi.org/10.1038/s41598-024-65542-yOlive varietiesISSRSCoTAnatomyGenetic diversityChemical constituents |
| spellingShingle | Haifa A. S. Alhaithloul Nabil S. Awad Sameer H. Qari Rania F. El-Homosy El-Sayed M. Qaoud Mesfer M. Alqahtani Kholoud Z. Ghanem Abdulrahman Alasmari Fahad M. Alzuaibr Hesham S. Ghazzawy Mohamed A. Abdein Genetic diversity, chemical constituents and anatomical analysis of eight popular Olive (Olea europaea L.) cultivars in Al-Jouf region, Saudi Arabia Scientific Reports Olive varieties ISSR SCoT Anatomy Genetic diversity Chemical constituents |
| title | Genetic diversity, chemical constituents and anatomical analysis of eight popular Olive (Olea europaea L.) cultivars in Al-Jouf region, Saudi Arabia |
| title_full | Genetic diversity, chemical constituents and anatomical analysis of eight popular Olive (Olea europaea L.) cultivars in Al-Jouf region, Saudi Arabia |
| title_fullStr | Genetic diversity, chemical constituents and anatomical analysis of eight popular Olive (Olea europaea L.) cultivars in Al-Jouf region, Saudi Arabia |
| title_full_unstemmed | Genetic diversity, chemical constituents and anatomical analysis of eight popular Olive (Olea europaea L.) cultivars in Al-Jouf region, Saudi Arabia |
| title_short | Genetic diversity, chemical constituents and anatomical analysis of eight popular Olive (Olea europaea L.) cultivars in Al-Jouf region, Saudi Arabia |
| title_sort | genetic diversity chemical constituents and anatomical analysis of eight popular olive olea europaea l cultivars in al jouf region saudi arabia |
| topic | Olive varieties ISSR SCoT Anatomy Genetic diversity Chemical constituents |
| url | https://doi.org/10.1038/s41598-024-65542-y |
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