Echinochrome A Increases Mitochondrial Mass and Function by Modulating Mitochondrial Biogenesis Regulatory Genes
Echinochrome A (Ech A) is a natural pigment from sea urchins that has been reported to have antioxidant properties and a cardio protective effect against ischemia reperfusion injury. In this study, we ascertained whether Ech A enhances the mitochondrial biogenesis and oxidative phosphorylation in ra...
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| Format: | Article |
| Language: | English |
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MDPI AG
2014-08-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/12/8/4602 |
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| author | Seung Hun Jeong Hyoung Kyu Kim In-Sung Song Su Jin Noh Jubert Marquez Kyung Soo Ko Byoung Doo Rhee Nari Kim Natalia P. Mishchenko Sergey A. Fedoreyev Valentin A. Stonik Jin Han |
| author_facet | Seung Hun Jeong Hyoung Kyu Kim In-Sung Song Su Jin Noh Jubert Marquez Kyung Soo Ko Byoung Doo Rhee Nari Kim Natalia P. Mishchenko Sergey A. Fedoreyev Valentin A. Stonik Jin Han |
| author_sort | Seung Hun Jeong |
| collection | DOAJ |
| description | Echinochrome A (Ech A) is a natural pigment from sea urchins that has been reported to have antioxidant properties and a cardio protective effect against ischemia reperfusion injury. In this study, we ascertained whether Ech A enhances the mitochondrial biogenesis and oxidative phosphorylation in rat cardio myoblast H9c2 cells. To study the effects of Ech A on mitochondrial biogenesis, we measured mitochondrial mass, level of oxidative phosphorylation, and mitochondrial biogenesis regulatory gene expression. Ech A treatment did not induce cytotoxicity. However, Ech A treatment enhanced oxygen consumption rate and mitochondrial ATP level. Likewise, Ech A treatment increased mitochondrial contents in H9c2 cells. Furthermore, Ech A treatment up-regulated biogenesis of regulatory transcription genes, including proliferator-activated receptor gamma co-activator (PGC)-1α, estrogen-related receptor (ERR)-α, peroxisome proliferator-activator receptor (PPAR)-γ, and nuclear respiratory factor (NRF)-1 and such mitochondrial transcription regulatory genes as mitochondrial transcriptional factor A (TFAM), mitochondrial transcription factor B2 (TFB2M), mitochondrial DNA direct polymerase (POLMRT), single strand binding protein (SSBP) and Tu translation elongation factor (TUFM). In conclusion, these data suggest that Ech A is a potentiated marine drug which enhances mitochondrial biogenesis. |
| first_indexed | 2024-04-11T13:02:05Z |
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| id | doaj.art-dba490ab848545a3a86688e2f7d2e461 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T13:02:05Z |
| publishDate | 2014-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-dba490ab848545a3a86688e2f7d2e4612022-12-22T04:22:54ZengMDPI AGMarine Drugs1660-33972014-08-011284602461510.3390/md12084602md12084602Echinochrome A Increases Mitochondrial Mass and Function by Modulating Mitochondrial Biogenesis Regulatory GenesSeung Hun Jeong0Hyoung Kyu Kim1In-Sung Song2Su Jin Noh3Jubert Marquez4Kyung Soo Ko5Byoung Doo Rhee6Nari Kim7Natalia P. Mishchenko8Sergey A. Fedoreyev9Valentin A. Stonik10Jin Han11Cardiovascular and Metabolic Disease Center (CMDC), National Research Laboratory for Mitochondrial Signaling, Inje University, Busan 614-735, KoreaCardiovascular and Metabolic Disease Center (CMDC), National Research Laboratory for Mitochondrial Signaling, Inje University, Busan 614-735, KoreaCardiovascular and Metabolic Disease Center (CMDC), National Research Laboratory for Mitochondrial Signaling, Inje University, Busan 614-735, KoreaDepartment of Health Sciences and Technology, Graduate School of Inje University, Busan 614-735, KoreaDepartment of Health Sciences and Technology, Graduate School of Inje University, Busan 614-735, KoreaCardiovascular and Metabolic Disease Center (CMDC), National Research Laboratory for Mitochondrial Signaling, Inje University, Busan 614-735, KoreaCardiovascular and Metabolic Disease Center (CMDC), National Research Laboratory for Mitochondrial Signaling, Inje University, Busan 614-735, KoreaCardiovascular and Metabolic Disease Center (CMDC), National Research Laboratory for Mitochondrial Signaling, Inje University, Busan 614-735, KoreaGeorge B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Science, Prospect 100 let Vladivostoku, 159, Vladivostok 690022, RussiaGeorge B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Science, Prospect 100 let Vladivostoku, 159, Vladivostok 690022, RussiaGeorge B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Science, Prospect 100 let Vladivostoku, 159, Vladivostok 690022, RussiaCardiovascular and Metabolic Disease Center (CMDC), National Research Laboratory for Mitochondrial Signaling, Inje University, Busan 614-735, KoreaEchinochrome A (Ech A) is a natural pigment from sea urchins that has been reported to have antioxidant properties and a cardio protective effect against ischemia reperfusion injury. In this study, we ascertained whether Ech A enhances the mitochondrial biogenesis and oxidative phosphorylation in rat cardio myoblast H9c2 cells. To study the effects of Ech A on mitochondrial biogenesis, we measured mitochondrial mass, level of oxidative phosphorylation, and mitochondrial biogenesis regulatory gene expression. Ech A treatment did not induce cytotoxicity. However, Ech A treatment enhanced oxygen consumption rate and mitochondrial ATP level. Likewise, Ech A treatment increased mitochondrial contents in H9c2 cells. Furthermore, Ech A treatment up-regulated biogenesis of regulatory transcription genes, including proliferator-activated receptor gamma co-activator (PGC)-1α, estrogen-related receptor (ERR)-α, peroxisome proliferator-activator receptor (PPAR)-γ, and nuclear respiratory factor (NRF)-1 and such mitochondrial transcription regulatory genes as mitochondrial transcriptional factor A (TFAM), mitochondrial transcription factor B2 (TFB2M), mitochondrial DNA direct polymerase (POLMRT), single strand binding protein (SSBP) and Tu translation elongation factor (TUFM). In conclusion, these data suggest that Ech A is a potentiated marine drug which enhances mitochondrial biogenesis.http://www.mdpi.com/1660-3397/12/8/4602echinochrome Amitochondrial biogenesisoxygen consumption rate |
| spellingShingle | Seung Hun Jeong Hyoung Kyu Kim In-Sung Song Su Jin Noh Jubert Marquez Kyung Soo Ko Byoung Doo Rhee Nari Kim Natalia P. Mishchenko Sergey A. Fedoreyev Valentin A. Stonik Jin Han Echinochrome A Increases Mitochondrial Mass and Function by Modulating Mitochondrial Biogenesis Regulatory Genes Marine Drugs echinochrome A mitochondrial biogenesis oxygen consumption rate |
| title | Echinochrome A Increases Mitochondrial Mass and Function by Modulating Mitochondrial Biogenesis Regulatory Genes |
| title_full | Echinochrome A Increases Mitochondrial Mass and Function by Modulating Mitochondrial Biogenesis Regulatory Genes |
| title_fullStr | Echinochrome A Increases Mitochondrial Mass and Function by Modulating Mitochondrial Biogenesis Regulatory Genes |
| title_full_unstemmed | Echinochrome A Increases Mitochondrial Mass and Function by Modulating Mitochondrial Biogenesis Regulatory Genes |
| title_short | Echinochrome A Increases Mitochondrial Mass and Function by Modulating Mitochondrial Biogenesis Regulatory Genes |
| title_sort | echinochrome a increases mitochondrial mass and function by modulating mitochondrial biogenesis regulatory genes |
| topic | echinochrome A mitochondrial biogenesis oxygen consumption rate |
| url | http://www.mdpi.com/1660-3397/12/8/4602 |
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