Evaluation of Histological Changes in Back Muscle Injuries in Rats over Time
Study DesignAnimal model study.PurposeThe purpose of this study was to evaluate the histological variation in the injured muscle and production of calcitonin gene-related peptide in rats over time.Overview of LiteratureVertebral surgery has been reported to cause atrophy of the back muscles, which m...
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| Format: | Article |
| Language: | English |
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Korean Spine Society
2017-02-01
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| Series: | Asian Spine Journal |
| Subjects: | |
| Online Access: | http://www.asianspinejournal.org/upload/pdf/asj-11-88.pdf |
| _version_ | 1818508056102699008 |
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| author | Koki Abe Kazuhide Inage Yoshihiro Sakuma Sumihisa Orita Kazuyo Yamauchi Miyako Suzuki Go Kubota Yasuhiro Oikawa Takeshi Sainoh Jun Sato Kazuki Fujimoto Yasuhiro Shiga Hirohito Kanamoto Kazuhisa Takahashi Seiji Ohtori |
| author_facet | Koki Abe Kazuhide Inage Yoshihiro Sakuma Sumihisa Orita Kazuyo Yamauchi Miyako Suzuki Go Kubota Yasuhiro Oikawa Takeshi Sainoh Jun Sato Kazuki Fujimoto Yasuhiro Shiga Hirohito Kanamoto Kazuhisa Takahashi Seiji Ohtori |
| author_sort | Koki Abe |
| collection | DOAJ |
| description | Study DesignAnimal model study.PurposeThe purpose of this study was to evaluate the histological variation in the injured muscle and production of calcitonin gene-related peptide in rats over time.Overview of LiteratureVertebral surgery has been reported to cause atrophy of the back muscles, which may result in pain. However, few reports have described the time series histological variation in the injured muscle and changes in the dominant nerve.MethodsWe used 30 male, 8-week-old Sprague-Dawley rats. The right and left sides of the paravertebral muscle were considered as the injured and uninjured sides, respectively. A 115 g weight was dropped from a height of 1 m on the right paravertebral muscle. Hematoxylin and eosin (H&E) staining of the muscle was performed 1–3 weeks after injury for histological evaluation. Fluoro-Gold (FG) was injected into the paravertebral muscle. The L2 dorsal root ganglia on both sides were resected 1, 2, and 3 weeks after injury, and immunohistochemical staining for calcitonin gene-related peptide was performed.ResultsH&E staining of the paravertebral muscle showed infiltration of inflammatory cells and the presence of granulation tissue in the injured part on the ipsilateral side 1 week after injury. Muscle atrophy occurred 3 weeks after injury, but was repaired via spontaneous replacement of muscle cells/fibers. In contrast, compared with the uninjured side, the percentage of cells double-labeled with FG and calcitonin gene-related peptide in FG-positive cells in the dorsal root ganglia of the injured side was significantly increased at each time point throughout the study period.ConclusionsThese results suggest that sensitization of the dominant nerve in the dorsal root ganglia, which may be caused by cicatrix formation, can protract injured muscle pain. This information may be helpful in elucidating the underlying mechanism of persistent pain after back muscle injury. |
| first_indexed | 2024-12-10T22:26:34Z |
| format | Article |
| id | doaj.art-dba649ecda354a7dbdda6f594a4bdd5c |
| institution | Directory Open Access Journal |
| issn | 1976-1902 1976-7846 |
| language | English |
| last_indexed | 2024-12-10T22:26:34Z |
| publishDate | 2017-02-01 |
| publisher | Korean Spine Society |
| record_format | Article |
| series | Asian Spine Journal |
| spelling | doaj.art-dba649ecda354a7dbdda6f594a4bdd5c2022-12-22T01:31:10ZengKorean Spine SocietyAsian Spine Journal1976-19021976-78462017-02-01111889210.4184/asj.2017.11.1.88302Evaluation of Histological Changes in Back Muscle Injuries in Rats over TimeKoki Abe0Kazuhide Inage1Yoshihiro Sakuma2Sumihisa Orita3Kazuyo Yamauchi4Miyako Suzuki5Go Kubota6Yasuhiro Oikawa7Takeshi Sainoh8Jun Sato9Kazuki Fujimoto10Yasuhiro Shiga11Hirohito Kanamoto12Kazuhisa Takahashi13Seiji Ohtori14Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.Department of Orthopaedic Surgery, National Hospital Organization, Chiba Medical Center, Chiba, Japan.Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.Department of Orthopaedic Surgery, Chiba Children's Hospital, Chiba, Japan.Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan.Study DesignAnimal model study.PurposeThe purpose of this study was to evaluate the histological variation in the injured muscle and production of calcitonin gene-related peptide in rats over time.Overview of LiteratureVertebral surgery has been reported to cause atrophy of the back muscles, which may result in pain. However, few reports have described the time series histological variation in the injured muscle and changes in the dominant nerve.MethodsWe used 30 male, 8-week-old Sprague-Dawley rats. The right and left sides of the paravertebral muscle were considered as the injured and uninjured sides, respectively. A 115 g weight was dropped from a height of 1 m on the right paravertebral muscle. Hematoxylin and eosin (H&E) staining of the muscle was performed 1–3 weeks after injury for histological evaluation. Fluoro-Gold (FG) was injected into the paravertebral muscle. The L2 dorsal root ganglia on both sides were resected 1, 2, and 3 weeks after injury, and immunohistochemical staining for calcitonin gene-related peptide was performed.ResultsH&E staining of the paravertebral muscle showed infiltration of inflammatory cells and the presence of granulation tissue in the injured part on the ipsilateral side 1 week after injury. Muscle atrophy occurred 3 weeks after injury, but was repaired via spontaneous replacement of muscle cells/fibers. In contrast, compared with the uninjured side, the percentage of cells double-labeled with FG and calcitonin gene-related peptide in FG-positive cells in the dorsal root ganglia of the injured side was significantly increased at each time point throughout the study period.ConclusionsThese results suggest that sensitization of the dominant nerve in the dorsal root ganglia, which may be caused by cicatrix formation, can protract injured muscle pain. This information may be helpful in elucidating the underlying mechanism of persistent pain after back muscle injury.http://www.asianspinejournal.org/upload/pdf/asj-11-88.pdfBack musclesSpineRatsCalcitonin gene-related peptideGanglia, sensory |
| spellingShingle | Koki Abe Kazuhide Inage Yoshihiro Sakuma Sumihisa Orita Kazuyo Yamauchi Miyako Suzuki Go Kubota Yasuhiro Oikawa Takeshi Sainoh Jun Sato Kazuki Fujimoto Yasuhiro Shiga Hirohito Kanamoto Kazuhisa Takahashi Seiji Ohtori Evaluation of Histological Changes in Back Muscle Injuries in Rats over Time Asian Spine Journal Back muscles Spine Rats Calcitonin gene-related peptide Ganglia, sensory |
| title | Evaluation of Histological Changes in Back Muscle Injuries in Rats over Time |
| title_full | Evaluation of Histological Changes in Back Muscle Injuries in Rats over Time |
| title_fullStr | Evaluation of Histological Changes in Back Muscle Injuries in Rats over Time |
| title_full_unstemmed | Evaluation of Histological Changes in Back Muscle Injuries in Rats over Time |
| title_short | Evaluation of Histological Changes in Back Muscle Injuries in Rats over Time |
| title_sort | evaluation of histological changes in back muscle injuries in rats over time |
| topic | Back muscles Spine Rats Calcitonin gene-related peptide Ganglia, sensory |
| url | http://www.asianspinejournal.org/upload/pdf/asj-11-88.pdf |
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