Identification and Protective Efficacy of <i>Eimeria tenella</i> Rhoptry Kinase Family Protein 17
<i>Eimeria tenella</i> encodes a genome of approximately 8000 genes. To date, however, very few data are available regarding <i>E. tenella</i> rhoptry kinase family proteins. In the present study, the gene fragment encoding the mature peptide of the rhoptry kinase family prot...
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2022-02-01
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| author | Xiaoxin Liu Bingjin Mu Wenbin Zheng Yijing Meng Linmei Yu Wenwei Gao Xingquan Zhu Qing Liu |
| author_facet | Xiaoxin Liu Bingjin Mu Wenbin Zheng Yijing Meng Linmei Yu Wenwei Gao Xingquan Zhu Qing Liu |
| author_sort | Xiaoxin Liu |
| collection | DOAJ |
| description | <i>Eimeria tenella</i> encodes a genome of approximately 8000 genes. To date, however, very few data are available regarding <i>E. tenella</i> rhoptry kinase family proteins. In the present study, the gene fragment encoding the mature peptide of the rhoptry kinase family protein 17 of <i>E. tenella</i> (EtROP17) was amplified by PCR and expressed in <i>E</i><i>. coli.</i> Then, we generated polyclonal antibodies that recognize EtROP17 and investigated the expression of EtROP17 in the merozoite stage of <i>E. tenella</i> by immunofluorescent staining and Western blot analysis. Meanwhile, the protective efficacy of rEtROP17 against <i>E. tenella</i> was evaluated in chickens. Sequencing analysis showed that a single base difference at sequence position 1901 was observed between the SD-01 strain and the Houghton strain. EtROP17 was expressed in the merozoite stage of <i>E. tenella.</i> The results of the animal challenge experiments demonstrated that vaccination with rEtROP17 significantly reduced cecal lesions and oocyst outputs compared with the challenged control group. Our findings indicate that EtROP17 could serve as a potential candidate for developing a new vaccine against <i>E. tenella</i>. |
| first_indexed | 2024-03-09T20:49:59Z |
| format | Article |
| id | doaj.art-dba880e64eec48c8a6acc89d23abbc23 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T20:49:59Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
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| spelling | doaj.art-dba880e64eec48c8a6acc89d23abbc232023-11-23T22:36:22ZengMDPI AGAnimals2076-26152022-02-0112555610.3390/ani12050556Identification and Protective Efficacy of <i>Eimeria tenella</i> Rhoptry Kinase Family Protein 17Xiaoxin Liu0Bingjin Mu1Wenbin Zheng2Yijing Meng3Linmei Yu4Wenwei Gao5Xingquan Zhu6Qing Liu7College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China<i>Eimeria tenella</i> encodes a genome of approximately 8000 genes. To date, however, very few data are available regarding <i>E. tenella</i> rhoptry kinase family proteins. In the present study, the gene fragment encoding the mature peptide of the rhoptry kinase family protein 17 of <i>E. tenella</i> (EtROP17) was amplified by PCR and expressed in <i>E</i><i>. coli.</i> Then, we generated polyclonal antibodies that recognize EtROP17 and investigated the expression of EtROP17 in the merozoite stage of <i>E. tenella</i> by immunofluorescent staining and Western blot analysis. Meanwhile, the protective efficacy of rEtROP17 against <i>E. tenella</i> was evaluated in chickens. Sequencing analysis showed that a single base difference at sequence position 1901 was observed between the SD-01 strain and the Houghton strain. EtROP17 was expressed in the merozoite stage of <i>E. tenella.</i> The results of the animal challenge experiments demonstrated that vaccination with rEtROP17 significantly reduced cecal lesions and oocyst outputs compared with the challenged control group. Our findings indicate that EtROP17 could serve as a potential candidate for developing a new vaccine against <i>E. tenella</i>.https://www.mdpi.com/2076-2615/12/5/556<i>Eimeria tenella</i>rhoptry kinase family protein 17merozoiteprotective efficacy |
| spellingShingle | Xiaoxin Liu Bingjin Mu Wenbin Zheng Yijing Meng Linmei Yu Wenwei Gao Xingquan Zhu Qing Liu Identification and Protective Efficacy of <i>Eimeria tenella</i> Rhoptry Kinase Family Protein 17 Animals <i>Eimeria tenella</i> rhoptry kinase family protein 17 merozoite protective efficacy |
| title | Identification and Protective Efficacy of <i>Eimeria tenella</i> Rhoptry Kinase Family Protein 17 |
| title_full | Identification and Protective Efficacy of <i>Eimeria tenella</i> Rhoptry Kinase Family Protein 17 |
| title_fullStr | Identification and Protective Efficacy of <i>Eimeria tenella</i> Rhoptry Kinase Family Protein 17 |
| title_full_unstemmed | Identification and Protective Efficacy of <i>Eimeria tenella</i> Rhoptry Kinase Family Protein 17 |
| title_short | Identification and Protective Efficacy of <i>Eimeria tenella</i> Rhoptry Kinase Family Protein 17 |
| title_sort | identification and protective efficacy of i eimeria tenella i rhoptry kinase family protein 17 |
| topic | <i>Eimeria tenella</i> rhoptry kinase family protein 17 merozoite protective efficacy |
| url | https://www.mdpi.com/2076-2615/12/5/556 |
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