Plasma Circulating mRNA Profile for the Non-Invasive Diagnosis of Colorectal Cancer Using NanoString Technologies
Colorectal cancer (CRC) is one of the most prevalent cancers and the second leading cause of cancer deaths in developed countries. Early CRC may have no symptoms and symptoms usually appear with more advanced diseases. Regular screening can identify people who are at increased risk of CRC in order t...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/5/3012 |
| _version_ | 1797264398901837824 |
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| author | Hin Fung Tsang Xiao Meng Pei Yin Kwan Evelyn Wong Sze Chuen Cesar Wong |
| author_facet | Hin Fung Tsang Xiao Meng Pei Yin Kwan Evelyn Wong Sze Chuen Cesar Wong |
| author_sort | Hin Fung Tsang |
| collection | DOAJ |
| description | Colorectal cancer (CRC) is one of the most prevalent cancers and the second leading cause of cancer deaths in developed countries. Early CRC may have no symptoms and symptoms usually appear with more advanced diseases. Regular screening can identify people who are at increased risk of CRC in order to offer earlier treatment. A cost-effective non-invasive platform for the screening and monitoring of CRC patients allows early detection and appropriate treatment of the disease, and the timely application of adjuvant therapy after surgical operation is needed. In this study, a cohort of 71 plasma samples that include 48 colonoscopy- and histopathology-confirmed CRC patients with TNM stages I to IV were recruited between 2017 and 2019. Plasma mRNA profiling was performed in CRC patients using NanoString nCounter. Normalized data were analyzed using a Mann–Whitney U test to determine statistically significant differences between samples from CRC patients and healthy subjects. A multiple-group comparison of clinical phenotypes was performed using the Kruskal–Wallis H test for statistically significant differences between multiple groups. Among the 27 selected circulating mRNA markers, all of them were found to be overexpressed (gene expression fold change > 2) in the plasma of patients from two or more CRC stages. In conclusion, NanoString-based targeted plasma CRC-associated mRNAs circulating the marker panel that can significantly distinguish CRC patients from a healthy population were developed for the non-invasive diagnosis of CRC using peripheral blood samples. |
| first_indexed | 2024-04-25T00:28:17Z |
| format | Article |
| id | doaj.art-dbab2cea5e34466e91332b930a503c72 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-04-25T00:28:17Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-dbab2cea5e34466e91332b930a503c722024-03-12T16:47:15ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-03-01255301210.3390/ijms25053012Plasma Circulating mRNA Profile for the Non-Invasive Diagnosis of Colorectal Cancer Using NanoString TechnologiesHin Fung Tsang0Xiao Meng Pei1Yin Kwan Evelyn Wong2Sze Chuen Cesar Wong3Department of Clinical Laboratory and Pathology, Hong Kong Adventist Hospital, Hong Kong SAR, ChinaDepartment of Applied Biology & Chemical Technology, The Hong Kong Polytechnic University, Hong Kong SAR, ChinaDepartment of Health Technology and Informatics, The Hong Kong Polytechnic University, Hong Kong SAR, ChinaDepartment of Applied Biology & Chemical Technology, The Hong Kong Polytechnic University, Hong Kong SAR, ChinaColorectal cancer (CRC) is one of the most prevalent cancers and the second leading cause of cancer deaths in developed countries. Early CRC may have no symptoms and symptoms usually appear with more advanced diseases. Regular screening can identify people who are at increased risk of CRC in order to offer earlier treatment. A cost-effective non-invasive platform for the screening and monitoring of CRC patients allows early detection and appropriate treatment of the disease, and the timely application of adjuvant therapy after surgical operation is needed. In this study, a cohort of 71 plasma samples that include 48 colonoscopy- and histopathology-confirmed CRC patients with TNM stages I to IV were recruited between 2017 and 2019. Plasma mRNA profiling was performed in CRC patients using NanoString nCounter. Normalized data were analyzed using a Mann–Whitney U test to determine statistically significant differences between samples from CRC patients and healthy subjects. A multiple-group comparison of clinical phenotypes was performed using the Kruskal–Wallis H test for statistically significant differences between multiple groups. Among the 27 selected circulating mRNA markers, all of them were found to be overexpressed (gene expression fold change > 2) in the plasma of patients from two or more CRC stages. In conclusion, NanoString-based targeted plasma CRC-associated mRNAs circulating the marker panel that can significantly distinguish CRC patients from a healthy population were developed for the non-invasive diagnosis of CRC using peripheral blood samples.https://www.mdpi.com/1422-0067/25/5/3012NanoStringnCounternon-invasive screeningplasma RNACRCcolorectal cancer |
| spellingShingle | Hin Fung Tsang Xiao Meng Pei Yin Kwan Evelyn Wong Sze Chuen Cesar Wong Plasma Circulating mRNA Profile for the Non-Invasive Diagnosis of Colorectal Cancer Using NanoString Technologies International Journal of Molecular Sciences NanoString nCounter non-invasive screening plasma RNA CRC colorectal cancer |
| title | Plasma Circulating mRNA Profile for the Non-Invasive Diagnosis of Colorectal Cancer Using NanoString Technologies |
| title_full | Plasma Circulating mRNA Profile for the Non-Invasive Diagnosis of Colorectal Cancer Using NanoString Technologies |
| title_fullStr | Plasma Circulating mRNA Profile for the Non-Invasive Diagnosis of Colorectal Cancer Using NanoString Technologies |
| title_full_unstemmed | Plasma Circulating mRNA Profile for the Non-Invasive Diagnosis of Colorectal Cancer Using NanoString Technologies |
| title_short | Plasma Circulating mRNA Profile for the Non-Invasive Diagnosis of Colorectal Cancer Using NanoString Technologies |
| title_sort | plasma circulating mrna profile for the non invasive diagnosis of colorectal cancer using nanostring technologies |
| topic | NanoString nCounter non-invasive screening plasma RNA CRC colorectal cancer |
| url | https://www.mdpi.com/1422-0067/25/5/3012 |
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