The effects of a highly bioavailable curcumin PhytosomeTM preparation on the retinal architecture and glial reactivity in the GFAP-IL6 mice
Uncontrolled, chronic inflammation in the retina can disturb retinal structure and function leading to impaired visual function. For the first time, in a mouse model of chronic neuroinflammation (GFAP-IL6), we investigated the impact of chronic glial activation on the retinal microglia population an...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-10-01
|
| Series: | Frontiers in Ophthalmology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fopht.2023.1205542/full |
| _version_ | 1797665732744445952 |
|---|---|
| author | Víctor Pérez-Fernández Akshaya Lakshmi Thananjeyan Faheem Ullah Faheem Ullah Gerald Münch Morven Cameron Erika Gyengesi |
| author_facet | Víctor Pérez-Fernández Akshaya Lakshmi Thananjeyan Faheem Ullah Faheem Ullah Gerald Münch Morven Cameron Erika Gyengesi |
| author_sort | Víctor Pérez-Fernández |
| collection | DOAJ |
| description | Uncontrolled, chronic inflammation in the retina can disturb retinal structure and function leading to impaired visual function. For the first time, in a mouse model of chronic neuroinflammation (GFAP-IL6), we investigated the impact of chronic glial activation on the retinal microglia population and structure. In addition, we tested a curcumin PhytosomeTM preparation with enhanced bioavailability to investigate the effects of a cytokine-suppressing anti-inflammatory drug on retinal architecture. Curcumin PhytosomeTM was fed to 3-month old GFAP-IL6 mice for 4 weeks and compared to their untreated GFAP-IL6 counterparts as well as wild type mice on control diet. Microglial numbers and morphology together with neuronal numbers were characterized using immunohistochemistry and cell reconstruction in the retina, using retinal wholemount and slices. GFAP-IL6 mice showed a significant increase in Iba1-labelled mononuclear phagocytes, including microglia, and displayed altered glial morphology. This resulted in a reduction in cone density and a thinning of the retinal layers compared to wild type mice. Curcumin PhytosomeTM treatment contributed to decreased microglial density, significantly decreasing both soma and cell size compared to control diet, as well as preventing the thinning of the retinal layers. This study is the first to characterize the impact of chronic retinal inflammation in the GFAP-IL6 mouse and the therapeutic benefit of enhanced bioavailable curcumin PhytosomeTM to significantly reduce microglia density and prevent neuronal loss. These data suggest that curcumin could be used as a complementary therapy alongside traditional treatments to reduce associated retinal inflammation in a variety of retinal diseases. |
| first_indexed | 2024-03-11T19:48:53Z |
| format | Article |
| id | doaj.art-dbae1896178e4157bf4744c66e5bb0cb |
| institution | Directory Open Access Journal |
| issn | 2674-0826 |
| language | English |
| last_indexed | 2024-03-11T19:48:53Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Ophthalmology |
| spelling | doaj.art-dbae1896178e4157bf4744c66e5bb0cb2023-10-05T15:45:19ZengFrontiers Media S.A.Frontiers in Ophthalmology2674-08262023-10-01310.3389/fopht.2023.12055421205542The effects of a highly bioavailable curcumin PhytosomeTM preparation on the retinal architecture and glial reactivity in the GFAP-IL6 miceVíctor Pérez-Fernández0Akshaya Lakshmi Thananjeyan1Faheem Ullah2Faheem Ullah3Gerald Münch4Morven Cameron5Erika Gyengesi6Department of Anatomy and Cell Biology, Western Sydney University, Campbelltown, NSW, AustraliaDepartment of Anatomy and Cell Biology, Western Sydney University, Campbelltown, NSW, AustraliaDepartment of Pharmacology, Western Sydney University, Campbelltown, NSW, AustraliaNeurosurgery, Robert Wood Johnson Medical School, Rutgers University, Piscataway, NJ, United StatesDepartment of Pharmacology, Western Sydney University, Campbelltown, NSW, AustraliaDepartment of Anatomy and Cell Biology, Western Sydney University, Campbelltown, NSW, AustraliaDepartment of Pharmacology, Western Sydney University, Campbelltown, NSW, AustraliaUncontrolled, chronic inflammation in the retina can disturb retinal structure and function leading to impaired visual function. For the first time, in a mouse model of chronic neuroinflammation (GFAP-IL6), we investigated the impact of chronic glial activation on the retinal microglia population and structure. In addition, we tested a curcumin PhytosomeTM preparation with enhanced bioavailability to investigate the effects of a cytokine-suppressing anti-inflammatory drug on retinal architecture. Curcumin PhytosomeTM was fed to 3-month old GFAP-IL6 mice for 4 weeks and compared to their untreated GFAP-IL6 counterparts as well as wild type mice on control diet. Microglial numbers and morphology together with neuronal numbers were characterized using immunohistochemistry and cell reconstruction in the retina, using retinal wholemount and slices. GFAP-IL6 mice showed a significant increase in Iba1-labelled mononuclear phagocytes, including microglia, and displayed altered glial morphology. This resulted in a reduction in cone density and a thinning of the retinal layers compared to wild type mice. Curcumin PhytosomeTM treatment contributed to decreased microglial density, significantly decreasing both soma and cell size compared to control diet, as well as preventing the thinning of the retinal layers. This study is the first to characterize the impact of chronic retinal inflammation in the GFAP-IL6 mouse and the therapeutic benefit of enhanced bioavailable curcumin PhytosomeTM to significantly reduce microglia density and prevent neuronal loss. These data suggest that curcumin could be used as a complementary therapy alongside traditional treatments to reduce associated retinal inflammation in a variety of retinal diseases.https://www.frontiersin.org/articles/10.3389/fopht.2023.1205542/fullanti-inflammatoryglial activationretinal morphologycurcumin PhytosomeTMretinal microglia |
| spellingShingle | Víctor Pérez-Fernández Akshaya Lakshmi Thananjeyan Faheem Ullah Faheem Ullah Gerald Münch Morven Cameron Erika Gyengesi The effects of a highly bioavailable curcumin PhytosomeTM preparation on the retinal architecture and glial reactivity in the GFAP-IL6 mice Frontiers in Ophthalmology anti-inflammatory glial activation retinal morphology curcumin PhytosomeTM retinal microglia |
| title | The effects of a highly bioavailable curcumin PhytosomeTM preparation on the retinal architecture and glial reactivity in the GFAP-IL6 mice |
| title_full | The effects of a highly bioavailable curcumin PhytosomeTM preparation on the retinal architecture and glial reactivity in the GFAP-IL6 mice |
| title_fullStr | The effects of a highly bioavailable curcumin PhytosomeTM preparation on the retinal architecture and glial reactivity in the GFAP-IL6 mice |
| title_full_unstemmed | The effects of a highly bioavailable curcumin PhytosomeTM preparation on the retinal architecture and glial reactivity in the GFAP-IL6 mice |
| title_short | The effects of a highly bioavailable curcumin PhytosomeTM preparation on the retinal architecture and glial reactivity in the GFAP-IL6 mice |
| title_sort | effects of a highly bioavailable curcumin phytosometm preparation on the retinal architecture and glial reactivity in the gfap il6 mice |
| topic | anti-inflammatory glial activation retinal morphology curcumin PhytosomeTM retinal microglia |
| url | https://www.frontiersin.org/articles/10.3389/fopht.2023.1205542/full |
| work_keys_str_mv | AT victorperezfernandez theeffectsofahighlybioavailablecurcuminphytosometmpreparationontheretinalarchitectureandglialreactivityinthegfapil6mice AT akshayalakshmithananjeyan theeffectsofahighlybioavailablecurcuminphytosometmpreparationontheretinalarchitectureandglialreactivityinthegfapil6mice AT faheemullah theeffectsofahighlybioavailablecurcuminphytosometmpreparationontheretinalarchitectureandglialreactivityinthegfapil6mice AT faheemullah theeffectsofahighlybioavailablecurcuminphytosometmpreparationontheretinalarchitectureandglialreactivityinthegfapil6mice AT geraldmunch theeffectsofahighlybioavailablecurcuminphytosometmpreparationontheretinalarchitectureandglialreactivityinthegfapil6mice AT morvencameron theeffectsofahighlybioavailablecurcuminphytosometmpreparationontheretinalarchitectureandglialreactivityinthegfapil6mice AT erikagyengesi theeffectsofahighlybioavailablecurcuminphytosometmpreparationontheretinalarchitectureandglialreactivityinthegfapil6mice AT victorperezfernandez effectsofahighlybioavailablecurcuminphytosometmpreparationontheretinalarchitectureandglialreactivityinthegfapil6mice AT akshayalakshmithananjeyan effectsofahighlybioavailablecurcuminphytosometmpreparationontheretinalarchitectureandglialreactivityinthegfapil6mice AT faheemullah effectsofahighlybioavailablecurcuminphytosometmpreparationontheretinalarchitectureandglialreactivityinthegfapil6mice AT faheemullah effectsofahighlybioavailablecurcuminphytosometmpreparationontheretinalarchitectureandglialreactivityinthegfapil6mice AT geraldmunch effectsofahighlybioavailablecurcuminphytosometmpreparationontheretinalarchitectureandglialreactivityinthegfapil6mice AT morvencameron effectsofahighlybioavailablecurcuminphytosometmpreparationontheretinalarchitectureandglialreactivityinthegfapil6mice AT erikagyengesi effectsofahighlybioavailablecurcuminphytosometmpreparationontheretinalarchitectureandglialreactivityinthegfapil6mice |