Effect of Topical Prostaglandin F2α Analogs on Selected Oxidative Stress Parameters in the Tear Film
<i>Background and Objectives</i>: Topically administered antiglaucoma medications, especially those containing benzalkonium chloride (BAC), may cause local adverse effects and compromise ocular surface. The aim of the study was to assess the effect of topical prostaglandin F2α a...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-07-01
|
| Series: | Medicina |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1010-660X/55/7/366 |
| _version_ | 1797710055924039680 |
|---|---|
| author | Lech Sedlak Maria Zych Weronika Wojnar Dorota Wyględowska-Promieńska |
| author_facet | Lech Sedlak Maria Zych Weronika Wojnar Dorota Wyględowska-Promieńska |
| author_sort | Lech Sedlak |
| collection | DOAJ |
| description | <i>Background and Objectives</i>: Topically administered antiglaucoma medications, especially those containing benzalkonium chloride (BAC), may cause local adverse effects and compromise ocular surface. The aim of the study was to assess the effect of topical prostaglandin F2α analogs (PGAs): preservative-free latanoprost, BAC-preserved latanoprost, preservative-free tafluprost, and BAC-preserved bimatoprost, on selected oxidative stress parameters in the tear film. <i>Materials and Methods</i>: The patients were divided into five groups: group C (<i>n</i> = 25) control group—subjects who did not use topical antiglaucoma medications, group L (<i>n</i> = 22)—patients using topical preservative-free latanoprost, group L+BAC (<i>n</i> = 25)—patients using topical BAC-preserved latanoprost, group T (<i>n</i> = 19)—patients using topical preservative-free tafluprost, and group B+BAC (<i>n</i> = 17)—patients using topical BAC-preserved bimatoprost. The oxidative stress markers in the tear film samples were evaluated: total protein (TP) concentration, advanced oxidation protein products (AOPP) content, total sulfhydryl (-SH) groups content, the activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), as well as Total Oxidant Status (TOS), Total Antioxidant Response (TAR), and Oxidative Stress Index (OSI). <i>Results</i>: The TP concentrations in the groups L, L+BAC, and B+BAC were statistically significantly higher in comparison with group C. The SOD and CAT activities in the groups L+BAC and B+BAC were statistically significantly higher when compared to group C. As compared to group C, AOPP and TOS were statistically significantly higher in all the study groups. OSI was found to be statistically significantly higher in the groups L+BAC, T, and B+BAC in comparison with group C. <i>Conclusion</i>: Use of topical PGAs by the patients with ocular hypertension or primary open-angle glaucoma is associated with increased oxidative stress in the tear film which is additionally exacerbated by the presence of BAC in the formulation. |
| first_indexed | 2024-03-12T06:46:38Z |
| format | Article |
| id | doaj.art-dbaecfc6b0ef411a8f5d8d9a264cc5d8 |
| institution | Directory Open Access Journal |
| issn | 1010-660X |
| language | English |
| last_indexed | 2024-03-12T06:46:38Z |
| publishDate | 2019-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Medicina |
| spelling | doaj.art-dbaecfc6b0ef411a8f5d8d9a264cc5d82023-09-03T00:33:11ZengMDPI AGMedicina1010-660X2019-07-0155736610.3390/medicina55070366medicina55070366Effect of Topical Prostaglandin F2α Analogs on Selected Oxidative Stress Parameters in the Tear FilmLech Sedlak0Maria Zych1Weronika Wojnar2Dorota Wyględowska-Promieńska3Department of Ophthalmology, School of Medicine in Katowice, Medical University of Silesia in Katowice, 40-514 Katowice, PolandDepartment of Pharmacognosy and Phytochemistry, School of Pharmacy with the Division of Laboratory Medicine, Medical University of Silesia in Katowice, 41-200 Sosnowiec, PolandDepartment of Pharmacognosy and Phytochemistry, School of Pharmacy with the Division of Laboratory Medicine, Medical University of Silesia in Katowice, 41-200 Sosnowiec, PolandDepartment of Ophthalmology, School of Medicine in Katowice, Medical University of Silesia in Katowice, 40-514 Katowice, Poland<i>Background and Objectives</i>: Topically administered antiglaucoma medications, especially those containing benzalkonium chloride (BAC), may cause local adverse effects and compromise ocular surface. The aim of the study was to assess the effect of topical prostaglandin F2α analogs (PGAs): preservative-free latanoprost, BAC-preserved latanoprost, preservative-free tafluprost, and BAC-preserved bimatoprost, on selected oxidative stress parameters in the tear film. <i>Materials and Methods</i>: The patients were divided into five groups: group C (<i>n</i> = 25) control group—subjects who did not use topical antiglaucoma medications, group L (<i>n</i> = 22)—patients using topical preservative-free latanoprost, group L+BAC (<i>n</i> = 25)—patients using topical BAC-preserved latanoprost, group T (<i>n</i> = 19)—patients using topical preservative-free tafluprost, and group B+BAC (<i>n</i> = 17)—patients using topical BAC-preserved bimatoprost. The oxidative stress markers in the tear film samples were evaluated: total protein (TP) concentration, advanced oxidation protein products (AOPP) content, total sulfhydryl (-SH) groups content, the activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), as well as Total Oxidant Status (TOS), Total Antioxidant Response (TAR), and Oxidative Stress Index (OSI). <i>Results</i>: The TP concentrations in the groups L, L+BAC, and B+BAC were statistically significantly higher in comparison with group C. The SOD and CAT activities in the groups L+BAC and B+BAC were statistically significantly higher when compared to group C. As compared to group C, AOPP and TOS were statistically significantly higher in all the study groups. OSI was found to be statistically significantly higher in the groups L+BAC, T, and B+BAC in comparison with group C. <i>Conclusion</i>: Use of topical PGAs by the patients with ocular hypertension or primary open-angle glaucoma is associated with increased oxidative stress in the tear film which is additionally exacerbated by the presence of BAC in the formulation.https://www.mdpi.com/1010-660X/55/7/366glaucomaocular surfacebenzalkonium chloridelatanoprosttafluprostbimatoprosttears |
| spellingShingle | Lech Sedlak Maria Zych Weronika Wojnar Dorota Wyględowska-Promieńska Effect of Topical Prostaglandin F2α Analogs on Selected Oxidative Stress Parameters in the Tear Film Medicina glaucoma ocular surface benzalkonium chloride latanoprost tafluprost bimatoprost tears |
| title | Effect of Topical Prostaglandin F2α Analogs on Selected Oxidative Stress Parameters in the Tear Film |
| title_full | Effect of Topical Prostaglandin F2α Analogs on Selected Oxidative Stress Parameters in the Tear Film |
| title_fullStr | Effect of Topical Prostaglandin F2α Analogs on Selected Oxidative Stress Parameters in the Tear Film |
| title_full_unstemmed | Effect of Topical Prostaglandin F2α Analogs on Selected Oxidative Stress Parameters in the Tear Film |
| title_short | Effect of Topical Prostaglandin F2α Analogs on Selected Oxidative Stress Parameters in the Tear Film |
| title_sort | effect of topical prostaglandin f2α analogs on selected oxidative stress parameters in the tear film |
| topic | glaucoma ocular surface benzalkonium chloride latanoprost tafluprost bimatoprost tears |
| url | https://www.mdpi.com/1010-660X/55/7/366 |
| work_keys_str_mv | AT lechsedlak effectoftopicalprostaglandinf2aanalogsonselectedoxidativestressparametersinthetearfilm AT mariazych effectoftopicalprostaglandinf2aanalogsonselectedoxidativestressparametersinthetearfilm AT weronikawojnar effectoftopicalprostaglandinf2aanalogsonselectedoxidativestressparametersinthetearfilm AT dorotawygledowskapromienska effectoftopicalprostaglandinf2aanalogsonselectedoxidativestressparametersinthetearfilm |