Antioxidant and Understanding the Anticancer Properties in Human Prostate and Breast Cancer Cell Lines of Chemically Characterized Methanol Extract from <i>Berberis hispanica</i> Boiss. & Reut
The current research was conducted to investigate the chemical profile, antiproliferative, and antioxidant activities of methanol extracts obtained by two different methods including maceration and Soxhlet from <i>Berberis hispanica</i> Boiss. & Reut. Antiproliferative activities wer...
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MDPI AG
2021-04-01
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| author | Loubna El Fakir Kaoutar Bouothmany Amal Alotaibi Mohammed Bourhia Riaz Ullah Saira Zahoor Mohamed El Mzibri Said Gmouh Tajelmolk Alaoui Abdelhamid Zaid Laila Benbacer |
| author_facet | Loubna El Fakir Kaoutar Bouothmany Amal Alotaibi Mohammed Bourhia Riaz Ullah Saira Zahoor Mohamed El Mzibri Said Gmouh Tajelmolk Alaoui Abdelhamid Zaid Laila Benbacer |
| author_sort | Loubna El Fakir |
| collection | DOAJ |
| description | The current research was conducted to investigate the chemical profile, antiproliferative, and antioxidant activities of methanol extracts obtained by two different methods including maceration and Soxhlet from <i>Berberis hispanica</i> Boiss. & Reut. Antiproliferative activities were evaluated by the MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay in four human cancer cell lines including prostate (LnCap and 22 RV1) and breast cancer (MDA-MB-231 and MCF7). The antioxidant power was evaluated by DPPH ((2,2-diphenyl-1-picryl-hydrazyl-hydrate), ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), and FRAPS (Ferric reducing antioxidant power) tests. The chemical composition was conducted by gas chromatography-mass spectrometry (GC-MS) after methylation. Total phenolic and flavonoid contents were assessed using the Folin–Ciocalteu method. The phytochemical analysis showed that the tested extracts possessed inserting potentially active compounds. The MTT test revealed that both extracts (maceration and Soxhlet) reduced cell viability in all cell lines tested. In breast cancer cell lines MDA-MB-231 and MCF-7, the IC<sub>50</sub> values obtained by maceration were 16.55 ± 0.58 and 17.95 ± 0.58 µg/mL, respectively. These values were slightly lower than those obtained with the Soxhlet extract toward MDA-MB-231 (19.93 ± 0.74 µg/mL) and MCF-7 (20.22 ± 0.89 µg/mL). Regarding prostate cancer cells 22 RV and LnCap, the IC<sub>50</sub> values obtained by maceration extract (22 RV: 11.75 ± 0.35 µg/mL; LnCap: 11.91 ± 0.54 µg/mL) were also slightly lower than those obtained with Soxhlet (22 RV: 13.47 ± 0.52 µg/mL; LnCap: 19.64 ± 1.05 µg/mL). The antioxidant activity showed that the studied extracts had considerable antioxidant activity (DPPH, FRAP, and ABTS) with particular attention to the extract obtained with maceration. The <i>Berberis hispanica</i> Bois. and Reut. can serve society as it provides potentially bioactive compounds that may find application in the medical sector to control such diseases. |
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| spelling | doaj.art-dbaf70a6df424c1eb2a38c4d14c6faab2023-11-21T15:32:34ZengMDPI AGApplied Sciences2076-34172021-04-01118351010.3390/app11083510Antioxidant and Understanding the Anticancer Properties in Human Prostate and Breast Cancer Cell Lines of Chemically Characterized Methanol Extract from <i>Berberis hispanica</i> Boiss. & ReutLoubna El Fakir0Kaoutar Bouothmany1Amal Alotaibi2Mohammed Bourhia3Riaz Ullah4Saira Zahoor5Mohamed El Mzibri6Said Gmouh7Tajelmolk Alaoui8Abdelhamid Zaid9Laila Benbacer10Laboratory of Management and Development of Natural Resources, Faculty of Sciences, University Moulay Ismail, Meknes B.P. 11201, MoroccoLaboratory of Pharmacology and Toxicology, Life Science Division, National Centre for Energy, Sciences, and Nuclear Techniques, Rabat B.P. 10001, MoroccoBasic Science Department, College of Medicine, Princess Nourah Bint Abdulrahman University, Riyadh 11564, Saudi ArabiaLaboratory of Chemistry-Biochemistry, Environment, Nutrition, and Health, Faculty of Medicine and Pharmacy, University Hassan II, Casablanca B.P. 5696, MoroccoDepartment of Pharmacognosy (MAPPRC), College of Pharmacy, King Saud University, Riyadh 11564, Saudi ArabiaDepartment of Pharmaceutics, College of Clinical Pharmacy, Imam Abdulrehman Bin Faisal University, P.O. Box 1982, Dammam 31441, Saudi ArabiaLaboratory of Pharmacology and Toxicology, Life Science Division, National Centre for Energy, Sciences, and Nuclear Techniques, Rabat B.P. 10001, MoroccoLaboratory of Engineering and Materials LIMAT, Faculty of Sciences Ben M’Sik, University Hassan II, Casablanca B.P. 7955, MoroccoLaboratory of Management and Development of Natural Resources, Faculty of Sciences, University Moulay Ismail, Meknes B.P. 11201, MoroccoLaboratory of Management and Development of Natural Resources, Faculty of Sciences, University Moulay Ismail, Meknes B.P. 11201, MoroccoLaboratory of Pharmacology and Toxicology, Life Science Division, National Centre for Energy, Sciences, and Nuclear Techniques, Rabat B.P. 10001, MoroccoThe current research was conducted to investigate the chemical profile, antiproliferative, and antioxidant activities of methanol extracts obtained by two different methods including maceration and Soxhlet from <i>Berberis hispanica</i> Boiss. & Reut. Antiproliferative activities were evaluated by the MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay in four human cancer cell lines including prostate (LnCap and 22 RV1) and breast cancer (MDA-MB-231 and MCF7). The antioxidant power was evaluated by DPPH ((2,2-diphenyl-1-picryl-hydrazyl-hydrate), ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), and FRAPS (Ferric reducing antioxidant power) tests. The chemical composition was conducted by gas chromatography-mass spectrometry (GC-MS) after methylation. Total phenolic and flavonoid contents were assessed using the Folin–Ciocalteu method. The phytochemical analysis showed that the tested extracts possessed inserting potentially active compounds. The MTT test revealed that both extracts (maceration and Soxhlet) reduced cell viability in all cell lines tested. In breast cancer cell lines MDA-MB-231 and MCF-7, the IC<sub>50</sub> values obtained by maceration were 16.55 ± 0.58 and 17.95 ± 0.58 µg/mL, respectively. These values were slightly lower than those obtained with the Soxhlet extract toward MDA-MB-231 (19.93 ± 0.74 µg/mL) and MCF-7 (20.22 ± 0.89 µg/mL). Regarding prostate cancer cells 22 RV and LnCap, the IC<sub>50</sub> values obtained by maceration extract (22 RV: 11.75 ± 0.35 µg/mL; LnCap: 11.91 ± 0.54 µg/mL) were also slightly lower than those obtained with Soxhlet (22 RV: 13.47 ± 0.52 µg/mL; LnCap: 19.64 ± 1.05 µg/mL). The antioxidant activity showed that the studied extracts had considerable antioxidant activity (DPPH, FRAP, and ABTS) with particular attention to the extract obtained with maceration. The <i>Berberis hispanica</i> Bois. and Reut. can serve society as it provides potentially bioactive compounds that may find application in the medical sector to control such diseases.https://www.mdpi.com/2076-3417/11/8/3510<i>Berberis hispanica</i> Bois. and Reut.antiproliferative activityGS-MSantioxidant activitycancer cell lines |
| spellingShingle | Loubna El Fakir Kaoutar Bouothmany Amal Alotaibi Mohammed Bourhia Riaz Ullah Saira Zahoor Mohamed El Mzibri Said Gmouh Tajelmolk Alaoui Abdelhamid Zaid Laila Benbacer Antioxidant and Understanding the Anticancer Properties in Human Prostate and Breast Cancer Cell Lines of Chemically Characterized Methanol Extract from <i>Berberis hispanica</i> Boiss. & Reut Applied Sciences <i>Berberis hispanica</i> Bois. and Reut. antiproliferative activity GS-MS antioxidant activity cancer cell lines |
| title | Antioxidant and Understanding the Anticancer Properties in Human Prostate and Breast Cancer Cell Lines of Chemically Characterized Methanol Extract from <i>Berberis hispanica</i> Boiss. & Reut |
| title_full | Antioxidant and Understanding the Anticancer Properties in Human Prostate and Breast Cancer Cell Lines of Chemically Characterized Methanol Extract from <i>Berberis hispanica</i> Boiss. & Reut |
| title_fullStr | Antioxidant and Understanding the Anticancer Properties in Human Prostate and Breast Cancer Cell Lines of Chemically Characterized Methanol Extract from <i>Berberis hispanica</i> Boiss. & Reut |
| title_full_unstemmed | Antioxidant and Understanding the Anticancer Properties in Human Prostate and Breast Cancer Cell Lines of Chemically Characterized Methanol Extract from <i>Berberis hispanica</i> Boiss. & Reut |
| title_short | Antioxidant and Understanding the Anticancer Properties in Human Prostate and Breast Cancer Cell Lines of Chemically Characterized Methanol Extract from <i>Berberis hispanica</i> Boiss. & Reut |
| title_sort | antioxidant and understanding the anticancer properties in human prostate and breast cancer cell lines of chemically characterized methanol extract from i berberis hispanica i boiss reut |
| topic | <i>Berberis hispanica</i> Bois. and Reut. antiproliferative activity GS-MS antioxidant activity cancer cell lines |
| url | https://www.mdpi.com/2076-3417/11/8/3510 |
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