Ovary of Zebrafish during Spawning Season: Ultrastructure and Immunohistochemical Profiles of Sox9 and Myostatin
This study sought to examine the ovarian cellular and stromal components of the zebrafish (<i>Danio rerio</i>) throughout the spawning season using light and electron microscopic tools. The ovaries of zebrafish showed oocytes in all stages of follicular development and degeneration (atre...
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MDPI AG
2023-10-01
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| Online Access: | https://www.mdpi.com/2076-2615/13/21/3362 |
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| author | Dalia Mohamedien Doaa M. Mokhtar Nada Abdellah Mahmoud Awad Marco Albano Ramy K. A. Sayed |
| author_facet | Dalia Mohamedien Doaa M. Mokhtar Nada Abdellah Mahmoud Awad Marco Albano Ramy K. A. Sayed |
| author_sort | Dalia Mohamedien |
| collection | DOAJ |
| description | This study sought to examine the ovarian cellular and stromal components of the zebrafish (<i>Danio rerio</i>) throughout the spawning season using light and electron microscopic tools. The ovaries of zebrafish showed oocytes in all stages of follicular development and degeneration (atresia). Six stages of oogenesis were demonstrated: oogonia, early oocytes, late oocytes, vacuolated follicles, the yolk globule stage (vitellogenesis), and mature follicles. The SOX9 protein was expressed in the ooplasm of the primary and previtellogenic oocytes and the theca cell layer of the mature follicles. Myostatin was expressed in the granulosa and theca cells. Many stem cells in the ovarian stroma expressed myostatin and SOX9. During the spawning season, the EM results indicated that the zona radiata increased in thickness and was crossed perpendicularly by pore canals that contained processes from both oocytes and zona granulosa. The granulosa cells contained many mitochondria, rER, sER, and vesicles. Meanwhile, the thecal layer consisted of fibroblast-like cells. Atretic follicles could be demonstrated that involved both oocytes and their follicular walls. Several types of cells were distinguished in the ovarian stroma, including mast cells, telocytes, lymphocytes, fibroblasts, endocrine cells, macrophages, adipocytes, dendritic cells, and steroidogenic (stromal) cells. The ovary of the zebrafish serves as a model to investigate follicular development. |
| first_indexed | 2024-03-11T11:35:08Z |
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| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T11:35:08Z |
| publishDate | 2023-10-01 |
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| spelling | doaj.art-dbb1c9ee1f9e4eda9698d19881c8b7b72023-11-10T14:57:51ZengMDPI AGAnimals2076-26152023-10-011321336210.3390/ani13213362Ovary of Zebrafish during Spawning Season: Ultrastructure and Immunohistochemical Profiles of Sox9 and MyostatinDalia Mohamedien0Doaa M. Mokhtar1Nada Abdellah2Mahmoud Awad3Marco Albano4Ramy K. A. Sayed5Department of Histology, Faculty of Veterinary Medicine, South Valley University, Qena 83523, EgyptDepartment of Cell and Tissues, Faculty of Veterinary Medicine, Assiut University, Assiut 71526, EgyptDepartment of Histology, Faculty of Veterinary Medicine, Sohag University, Sohag 82524, EgyptDepartment of Histology, Faculty of Veterinary Medicine, South Valley University, Qena 83523, EgyptDepartment of Veterinary Sciences, University of Messina, Polo Universitario Dell’Annunziata, 98168 Messina, ItalyDepartment of Anatomy and Embryology, Faculty of Veterinary Medicine, Sohag University, Sohag 82524, EgyptThis study sought to examine the ovarian cellular and stromal components of the zebrafish (<i>Danio rerio</i>) throughout the spawning season using light and electron microscopic tools. The ovaries of zebrafish showed oocytes in all stages of follicular development and degeneration (atresia). Six stages of oogenesis were demonstrated: oogonia, early oocytes, late oocytes, vacuolated follicles, the yolk globule stage (vitellogenesis), and mature follicles. The SOX9 protein was expressed in the ooplasm of the primary and previtellogenic oocytes and the theca cell layer of the mature follicles. Myostatin was expressed in the granulosa and theca cells. Many stem cells in the ovarian stroma expressed myostatin and SOX9. During the spawning season, the EM results indicated that the zona radiata increased in thickness and was crossed perpendicularly by pore canals that contained processes from both oocytes and zona granulosa. The granulosa cells contained many mitochondria, rER, sER, and vesicles. Meanwhile, the thecal layer consisted of fibroblast-like cells. Atretic follicles could be demonstrated that involved both oocytes and their follicular walls. Several types of cells were distinguished in the ovarian stroma, including mast cells, telocytes, lymphocytes, fibroblasts, endocrine cells, macrophages, adipocytes, dendritic cells, and steroidogenic (stromal) cells. The ovary of the zebrafish serves as a model to investigate follicular development.https://www.mdpi.com/2076-2615/13/21/3362theca cellsovarian follicleselectron microscopyoogenesisovarian stroma |
| spellingShingle | Dalia Mohamedien Doaa M. Mokhtar Nada Abdellah Mahmoud Awad Marco Albano Ramy K. A. Sayed Ovary of Zebrafish during Spawning Season: Ultrastructure and Immunohistochemical Profiles of Sox9 and Myostatin Animals theca cells ovarian follicles electron microscopy oogenesis ovarian stroma |
| title | Ovary of Zebrafish during Spawning Season: Ultrastructure and Immunohistochemical Profiles of Sox9 and Myostatin |
| title_full | Ovary of Zebrafish during Spawning Season: Ultrastructure and Immunohistochemical Profiles of Sox9 and Myostatin |
| title_fullStr | Ovary of Zebrafish during Spawning Season: Ultrastructure and Immunohistochemical Profiles of Sox9 and Myostatin |
| title_full_unstemmed | Ovary of Zebrafish during Spawning Season: Ultrastructure and Immunohistochemical Profiles of Sox9 and Myostatin |
| title_short | Ovary of Zebrafish during Spawning Season: Ultrastructure and Immunohistochemical Profiles of Sox9 and Myostatin |
| title_sort | ovary of zebrafish during spawning season ultrastructure and immunohistochemical profiles of sox9 and myostatin |
| topic | theca cells ovarian follicles electron microscopy oogenesis ovarian stroma |
| url | https://www.mdpi.com/2076-2615/13/21/3362 |
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