Safe Pseudovirus-based Assay for Neutralization Antibodies against Influenza A(H7N9) Virus

Serologic studies are urgently needed to assist in understanding an outbreak of influenza A(H7N9) virus. However, a biosafety level 3 laboratory is required for conventional serologic assays with live lethal virus. We describe a safe pseudovirus–based neutralization assay with preliminary assessment...

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Main Authors: Chao Qiu, Yang Huang, Anli Zhang, Di Tian, Yanmin Wan, Xiaoling Zhang, Wanju Zhang, Zhiyong Zhang, Zhenghong Yuan, Yunwen Hu, Xiaoyan Zhang, Jianqing Xu
Format: Article
Language:English
Published: Centers for Disease Control and Prevention 2013-10-01
Series:Emerging Infectious Diseases
Subjects:
Online Access:https://wwwnc.cdc.gov/eid/article/19/10/13-0728_article
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author Chao Qiu
Yang Huang
Anli Zhang
Di Tian
Yanmin Wan
Xiaoling Zhang
Wanju Zhang
Zhiyong Zhang
Zhenghong Yuan
Yunwen Hu
Xiaoyan Zhang
Jianqing Xu
author_facet Chao Qiu
Yang Huang
Anli Zhang
Di Tian
Yanmin Wan
Xiaoling Zhang
Wanju Zhang
Zhiyong Zhang
Zhenghong Yuan
Yunwen Hu
Xiaoyan Zhang
Jianqing Xu
author_sort Chao Qiu
collection DOAJ
description Serologic studies are urgently needed to assist in understanding an outbreak of influenza A(H7N9) virus. However, a biosafety level 3 laboratory is required for conventional serologic assays with live lethal virus. We describe a safe pseudovirus–based neutralization assay with preliminary assessment using subtype H7N9–infected samples and controls.
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1080-6059
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publishDate 2013-10-01
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spelling doaj.art-dbb27cdc8905430388c8ac640c6930412022-12-21T18:18:10ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592013-10-0119101685168710.3201/eid1910.130728Safe Pseudovirus-based Assay for Neutralization Antibodies against Influenza A(H7N9) VirusChao QiuYang HuangAnli ZhangDi TianYanmin WanXiaoling ZhangWanju ZhangZhiyong ZhangZhenghong YuanYunwen HuXiaoyan ZhangJianqing XuSerologic studies are urgently needed to assist in understanding an outbreak of influenza A(H7N9) virus. However, a biosafety level 3 laboratory is required for conventional serologic assays with live lethal virus. We describe a safe pseudovirus–based neutralization assay with preliminary assessment using subtype H7N9–infected samples and controls.https://wwwnc.cdc.gov/eid/article/19/10/13-0728_articleInfluenzasubtype H7N9serologyneutralization testspseudovirusviruses
spellingShingle Chao Qiu
Yang Huang
Anli Zhang
Di Tian
Yanmin Wan
Xiaoling Zhang
Wanju Zhang
Zhiyong Zhang
Zhenghong Yuan
Yunwen Hu
Xiaoyan Zhang
Jianqing Xu
Safe Pseudovirus-based Assay for Neutralization Antibodies against Influenza A(H7N9) Virus
Emerging Infectious Diseases
Influenza
subtype H7N9
serology
neutralization tests
pseudovirus
viruses
title Safe Pseudovirus-based Assay for Neutralization Antibodies against Influenza A(H7N9) Virus
title_full Safe Pseudovirus-based Assay for Neutralization Antibodies against Influenza A(H7N9) Virus
title_fullStr Safe Pseudovirus-based Assay for Neutralization Antibodies against Influenza A(H7N9) Virus
title_full_unstemmed Safe Pseudovirus-based Assay for Neutralization Antibodies against Influenza A(H7N9) Virus
title_short Safe Pseudovirus-based Assay for Neutralization Antibodies against Influenza A(H7N9) Virus
title_sort safe pseudovirus based assay for neutralization antibodies against influenza a h7n9 virus
topic Influenza
subtype H7N9
serology
neutralization tests
pseudovirus
viruses
url https://wwwnc.cdc.gov/eid/article/19/10/13-0728_article
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