Detection of SARS-CoV-2 in Neonatal Autopsy Tissues and Placenta
Severe coronavirus disease in neonates is rare. We analyzed clinical, laboratory, and autopsy findings from a neonate in the United States who was delivered at 25 weeks of gestation and died 4 days after birth; the mother had asymptomatic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Centers for Disease Control and Prevention
2022-03-01
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| Series: | Emerging Infectious Diseases |
| Subjects: | |
| Online Access: | https://wwwnc.cdc.gov/eid/article/28/3/21-1735_article |
| _version_ | 1819278212441571328 |
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| author | Sarah Reagan-Steiner Julu Bhatnagar Roosecelis B. Martines Nicholas S. Milligan Carly Gisondo Frank B. Williams Elizabeth Lee Lindsey Estetter Hannah Bullock Cynthia S. Goldsmith Pamela Fair Julie Hand Gillian Richardson Kate R. Woodworth Titilope Oduyebo Romeo R. Galang Rebecca Phillips Elizaveta Belyaeva Xiao-Ming Yin Dana Meaney-Delman Timothy M. Uyeki Drucilla J. Roberts Sherif R. Zaki |
| author_facet | Sarah Reagan-Steiner Julu Bhatnagar Roosecelis B. Martines Nicholas S. Milligan Carly Gisondo Frank B. Williams Elizabeth Lee Lindsey Estetter Hannah Bullock Cynthia S. Goldsmith Pamela Fair Julie Hand Gillian Richardson Kate R. Woodworth Titilope Oduyebo Romeo R. Galang Rebecca Phillips Elizaveta Belyaeva Xiao-Ming Yin Dana Meaney-Delman Timothy M. Uyeki Drucilla J. Roberts Sherif R. Zaki |
| author_sort | Sarah Reagan-Steiner |
| collection | DOAJ |
| description |
Severe coronavirus disease in neonates is rare. We analyzed clinical, laboratory, and autopsy findings from a neonate in the United States who was delivered at 25 weeks of gestation and died 4 days after birth; the mother had asymptomatic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and preeclampsia. We observed severe diffuse alveolar damage and localized SARS-CoV-2 by immunohistochemistry, in situ hybridization, and electron microscopy of the lungs of the neonate. We localized SARS-CoV-2 RNA in neonatal heart and liver vascular endothelium by using in situ hybridization and detected SARS-CoV-2 RNA in neonatal and placental tissues by using reverse transcription PCR. Subgenomic reverse transcription PCR suggested viral replication in lung/airway, heart, and liver. These findings indicate that in utero SARS-CoV-2 transmission contributed to this neonatal death.
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| first_indexed | 2024-12-24T00:08:25Z |
| format | Article |
| id | doaj.art-dbba9e5d326d4e7fb088350a577698de |
| institution | Directory Open Access Journal |
| issn | 1080-6040 1080-6059 |
| language | English |
| last_indexed | 2024-12-24T00:08:25Z |
| publishDate | 2022-03-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj.art-dbba9e5d326d4e7fb088350a577698de2022-12-21T17:24:56ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592022-03-0128351051710.3201/eid2803.211735Detection of SARS-CoV-2 in Neonatal Autopsy Tissues and PlacentaSarah Reagan-SteinerJulu BhatnagarRoosecelis B. MartinesNicholas S. MilliganCarly GisondoFrank B. WilliamsElizabeth LeeLindsey EstetterHannah BullockCynthia S. GoldsmithPamela FairJulie HandGillian RichardsonKate R. WoodworthTitilope OduyeboRomeo R. GalangRebecca PhillipsElizaveta BelyaevaXiao-Ming YinDana Meaney-DelmanTimothy M. UyekiDrucilla J. RobertsSherif R. Zaki Severe coronavirus disease in neonates is rare. We analyzed clinical, laboratory, and autopsy findings from a neonate in the United States who was delivered at 25 weeks of gestation and died 4 days after birth; the mother had asymptomatic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and preeclampsia. We observed severe diffuse alveolar damage and localized SARS-CoV-2 by immunohistochemistry, in situ hybridization, and electron microscopy of the lungs of the neonate. We localized SARS-CoV-2 RNA in neonatal heart and liver vascular endothelium by using in situ hybridization and detected SARS-CoV-2 RNA in neonatal and placental tissues by using reverse transcription PCR. Subgenomic reverse transcription PCR suggested viral replication in lung/airway, heart, and liver. These findings indicate that in utero SARS-CoV-2 transmission contributed to this neonatal death. https://wwwnc.cdc.gov/eid/article/28/3/21-1735_articleCOVID-192019 novel coronavirus diseasecoronavirus diseasesevere acute respiratory syndrome coronavirus 2SARS-CoV-2vertical transmission |
| spellingShingle | Sarah Reagan-Steiner Julu Bhatnagar Roosecelis B. Martines Nicholas S. Milligan Carly Gisondo Frank B. Williams Elizabeth Lee Lindsey Estetter Hannah Bullock Cynthia S. Goldsmith Pamela Fair Julie Hand Gillian Richardson Kate R. Woodworth Titilope Oduyebo Romeo R. Galang Rebecca Phillips Elizaveta Belyaeva Xiao-Ming Yin Dana Meaney-Delman Timothy M. Uyeki Drucilla J. Roberts Sherif R. Zaki Detection of SARS-CoV-2 in Neonatal Autopsy Tissues and Placenta Emerging Infectious Diseases COVID-19 2019 novel coronavirus disease coronavirus disease severe acute respiratory syndrome coronavirus 2 SARS-CoV-2 vertical transmission |
| title | Detection of SARS-CoV-2 in Neonatal Autopsy Tissues and Placenta |
| title_full | Detection of SARS-CoV-2 in Neonatal Autopsy Tissues and Placenta |
| title_fullStr | Detection of SARS-CoV-2 in Neonatal Autopsy Tissues and Placenta |
| title_full_unstemmed | Detection of SARS-CoV-2 in Neonatal Autopsy Tissues and Placenta |
| title_short | Detection of SARS-CoV-2 in Neonatal Autopsy Tissues and Placenta |
| title_sort | detection of sars cov 2 in neonatal autopsy tissues and placenta |
| topic | COVID-19 2019 novel coronavirus disease coronavirus disease severe acute respiratory syndrome coronavirus 2 SARS-CoV-2 vertical transmission |
| url | https://wwwnc.cdc.gov/eid/article/28/3/21-1735_article |
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