Aqueous extract of Artemisia capillaris improves non-alcoholic fatty liver and obesity in mice induced by high-fat diet
Non-alcoholic fatty liver disease (NAFLD) is one of the most common chronic liver diseases and is a nutritional metabolic disease. Artemisia capillaris (AC) is the above-ground dried part of Artemisia capillaris Thunb. or Artemisia scoparia Waldst. et Kit., a natural medicinal plant with pharmacolog...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2022.1084435/full |
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| author | Meng Liang Mohan Huo Yi Guo Yuyi Zhang Xiao Xiao Jianwen Xv Lixue Fang Tianqi Li Huan Wang Siyu Dong Xiaowen Jiang Wenhui Yu Wenhui Yu Wenhui Yu |
| author_facet | Meng Liang Mohan Huo Yi Guo Yuyi Zhang Xiao Xiao Jianwen Xv Lixue Fang Tianqi Li Huan Wang Siyu Dong Xiaowen Jiang Wenhui Yu Wenhui Yu Wenhui Yu |
| author_sort | Meng Liang |
| collection | DOAJ |
| description | Non-alcoholic fatty liver disease (NAFLD) is one of the most common chronic liver diseases and is a nutritional metabolic disease. Artemisia capillaris (AC) is the above-ground dried part of Artemisia capillaris Thunb. or Artemisia scoparia Waldst. et Kit., a natural medicinal plant with pharmacological effects of heat-clearing and biliary-promoting. In order to evaluate the therapeutic effect of Artemisia capillaris on NAFLD and obesity, experiments were conducted using aqueous extracts of Artemisia capillaris (WAC) to intervene in NAFLD models in vivo and in vitro. In vivo experiments were performed using HFD-fed (high fat diet) C57BL/6 mice to induce NAFLD model, and in vitro experiments were performed using oleic acid to induce HepG2 cells to construct NAFLD cell model. H.E. staining and oil red O staining of liver tissue were used to observe hepatocytes. Blood biochemistry analyzer was used to detect serum lipid levels in mice. The drug targets and mechanism of action of AC to improve NAFLD were investigated by western blotting, qRT-PCR and immunofluorescence. The results showed that C57BL/6 mice fed HFD continuously for 16 weeks met the criteria for NAFLD in terms of lipid index and hepatocyte fat accumulation. WAC was able to reverse the elevation of serum lipid levels induced by high-fat diet in mice. WAC promoted the phosphorylation levels of PI3K/AKT and AMPK in liver and HepG2 cells of NAFLD mice, inhibited SREBP-1c expression, reduced TG and lipogenesis, and decreased lipid accumulation. In summary, WAC extract activates PI3K/AKT pathway, reduces SREBP-1c protein expression by promoting AMPK phosphorylation, and decreases fatty acid synthesis and TG content in hepatocytes. AC can be used as a potential health herb to improve NAFLD and obesity. |
| first_indexed | 2024-04-11T13:53:13Z |
| format | Article |
| id | doaj.art-dbbb19709aad414fa07c78b2ae71b188 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-11T13:53:13Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-dbbb19709aad414fa07c78b2ae71b1882022-12-22T04:20:29ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122022-11-011310.3389/fphar.2022.10844351084435Aqueous extract of Artemisia capillaris improves non-alcoholic fatty liver and obesity in mice induced by high-fat dietMeng Liang0Mohan Huo1Yi Guo2Yuyi Zhang3Xiao Xiao4Jianwen Xv5Lixue Fang6Tianqi Li7Huan Wang8Siyu Dong9Xiaowen Jiang10Wenhui Yu11Wenhui Yu12Wenhui Yu13Department of Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaDepartment of Life Sciences, Northeast Agricultural University, Harbin, ChinaDepartment of Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaDepartment of Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaDepartment of Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaDepartment of Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaDepartment of Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaDepartment of Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaDepartment of Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaDepartment of Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaDepartment of Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaDepartment of Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaInstitute of Chinese Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaHeilongjiang Provincial Key Laboratory for Prevention and Control of Common Animal Diseases, Northeast Agricultural University, Harbin, ChinaNon-alcoholic fatty liver disease (NAFLD) is one of the most common chronic liver diseases and is a nutritional metabolic disease. Artemisia capillaris (AC) is the above-ground dried part of Artemisia capillaris Thunb. or Artemisia scoparia Waldst. et Kit., a natural medicinal plant with pharmacological effects of heat-clearing and biliary-promoting. In order to evaluate the therapeutic effect of Artemisia capillaris on NAFLD and obesity, experiments were conducted using aqueous extracts of Artemisia capillaris (WAC) to intervene in NAFLD models in vivo and in vitro. In vivo experiments were performed using HFD-fed (high fat diet) C57BL/6 mice to induce NAFLD model, and in vitro experiments were performed using oleic acid to induce HepG2 cells to construct NAFLD cell model. H.E. staining and oil red O staining of liver tissue were used to observe hepatocytes. Blood biochemistry analyzer was used to detect serum lipid levels in mice. The drug targets and mechanism of action of AC to improve NAFLD were investigated by western blotting, qRT-PCR and immunofluorescence. The results showed that C57BL/6 mice fed HFD continuously for 16 weeks met the criteria for NAFLD in terms of lipid index and hepatocyte fat accumulation. WAC was able to reverse the elevation of serum lipid levels induced by high-fat diet in mice. WAC promoted the phosphorylation levels of PI3K/AKT and AMPK in liver and HepG2 cells of NAFLD mice, inhibited SREBP-1c expression, reduced TG and lipogenesis, and decreased lipid accumulation. In summary, WAC extract activates PI3K/AKT pathway, reduces SREBP-1c protein expression by promoting AMPK phosphorylation, and decreases fatty acid synthesis and TG content in hepatocytes. AC can be used as a potential health herb to improve NAFLD and obesity.https://www.frontiersin.org/articles/10.3389/fphar.2022.1084435/fullaqueous extracts of Artemisia capillarisNAFLDlipid metabolismPI3K/AKTAMPKSREBP-1c |
| spellingShingle | Meng Liang Mohan Huo Yi Guo Yuyi Zhang Xiao Xiao Jianwen Xv Lixue Fang Tianqi Li Huan Wang Siyu Dong Xiaowen Jiang Wenhui Yu Wenhui Yu Wenhui Yu Aqueous extract of Artemisia capillaris improves non-alcoholic fatty liver and obesity in mice induced by high-fat diet Frontiers in Pharmacology aqueous extracts of Artemisia capillaris NAFLD lipid metabolism PI3K/AKT AMPK SREBP-1c |
| title | Aqueous extract of Artemisia capillaris improves non-alcoholic fatty liver and obesity in mice induced by high-fat diet |
| title_full | Aqueous extract of Artemisia capillaris improves non-alcoholic fatty liver and obesity in mice induced by high-fat diet |
| title_fullStr | Aqueous extract of Artemisia capillaris improves non-alcoholic fatty liver and obesity in mice induced by high-fat diet |
| title_full_unstemmed | Aqueous extract of Artemisia capillaris improves non-alcoholic fatty liver and obesity in mice induced by high-fat diet |
| title_short | Aqueous extract of Artemisia capillaris improves non-alcoholic fatty liver and obesity in mice induced by high-fat diet |
| title_sort | aqueous extract of artemisia capillaris improves non alcoholic fatty liver and obesity in mice induced by high fat diet |
| topic | aqueous extracts of Artemisia capillaris NAFLD lipid metabolism PI3K/AKT AMPK SREBP-1c |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2022.1084435/full |
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