Cold-Active Lipases and Esterases: A Review on Recombinant Overexpression and Other Essential Issues
Cold environments characterised by diverse temperatures close to or below the water freezing point dominate about 80% of the Earth’s biosphere. One of the survival strategies adopted by microorganisms living in cold environments is their expression of cold-active enzymes that enable them to perform...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/23/15394 |
| _version_ | 1797463034478723072 |
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| author | Adamu Idris Matinja Nor Hafizah Ahmad Kamarudin Adam Thean Chor Leow Siti Nurbaya Oslan Mohd Shukuri Mohamad Ali |
| author_facet | Adamu Idris Matinja Nor Hafizah Ahmad Kamarudin Adam Thean Chor Leow Siti Nurbaya Oslan Mohd Shukuri Mohamad Ali |
| author_sort | Adamu Idris Matinja |
| collection | DOAJ |
| description | Cold environments characterised by diverse temperatures close to or below the water freezing point dominate about 80% of the Earth’s biosphere. One of the survival strategies adopted by microorganisms living in cold environments is their expression of cold-active enzymes that enable them to perform an efficient metabolic flux at low temperatures necessary to thrive and reproduce under those constraints. Cold-active enzymes are ideal biocatalysts that can reduce the need for heating procedures and improve industrial processes’ quality, sustainability, and cost-effectiveness. Despite their wide applications, their industrial usage is still limited, and the major contributing factor is the lack of complete understanding of their structure and cold adaptation mechanisms. The current review looked at the recombinant overexpression, purification, and recent mechanism of cold adaptation, various approaches for purification, and three-dimensional (3D) crystal structure elucidation of cold-active lipases and esterase. |
| first_indexed | 2024-03-09T17:44:58Z |
| format | Article |
| id | doaj.art-dbbd2547871c4c098fa9626462f27596 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T17:44:58Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-dbbd2547871c4c098fa9626462f275962023-11-24T11:18:17ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-12-0123231539410.3390/ijms232315394Cold-Active Lipases and Esterases: A Review on Recombinant Overexpression and Other Essential IssuesAdamu Idris Matinja0Nor Hafizah Ahmad Kamarudin1Adam Thean Chor Leow2Siti Nurbaya Oslan3Mohd Shukuri Mohamad Ali4Enzyme and Microbial Technology Research Centre, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, MalaysiaEnzyme and Microbial Technology Research Centre, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, MalaysiaEnzyme and Microbial Technology Research Centre, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, MalaysiaEnzyme and Microbial Technology Research Centre, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, MalaysiaEnzyme and Microbial Technology Research Centre, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, MalaysiaCold environments characterised by diverse temperatures close to or below the water freezing point dominate about 80% of the Earth’s biosphere. One of the survival strategies adopted by microorganisms living in cold environments is their expression of cold-active enzymes that enable them to perform an efficient metabolic flux at low temperatures necessary to thrive and reproduce under those constraints. Cold-active enzymes are ideal biocatalysts that can reduce the need for heating procedures and improve industrial processes’ quality, sustainability, and cost-effectiveness. Despite their wide applications, their industrial usage is still limited, and the major contributing factor is the lack of complete understanding of their structure and cold adaptation mechanisms. The current review looked at the recombinant overexpression, purification, and recent mechanism of cold adaptation, various approaches for purification, and three-dimensional (3D) crystal structure elucidation of cold-active lipases and esterase.https://www.mdpi.com/1422-0067/23/23/15394cold adaptation3D structureesteraselipasepsychrophilic enzymespurification |
| spellingShingle | Adamu Idris Matinja Nor Hafizah Ahmad Kamarudin Adam Thean Chor Leow Siti Nurbaya Oslan Mohd Shukuri Mohamad Ali Cold-Active Lipases and Esterases: A Review on Recombinant Overexpression and Other Essential Issues International Journal of Molecular Sciences cold adaptation 3D structure esterase lipase psychrophilic enzymes purification |
| title | Cold-Active Lipases and Esterases: A Review on Recombinant Overexpression and Other Essential Issues |
| title_full | Cold-Active Lipases and Esterases: A Review on Recombinant Overexpression and Other Essential Issues |
| title_fullStr | Cold-Active Lipases and Esterases: A Review on Recombinant Overexpression and Other Essential Issues |
| title_full_unstemmed | Cold-Active Lipases and Esterases: A Review on Recombinant Overexpression and Other Essential Issues |
| title_short | Cold-Active Lipases and Esterases: A Review on Recombinant Overexpression and Other Essential Issues |
| title_sort | cold active lipases and esterases a review on recombinant overexpression and other essential issues |
| topic | cold adaptation 3D structure esterase lipase psychrophilic enzymes purification |
| url | https://www.mdpi.com/1422-0067/23/23/15394 |
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