Determination of trace elements in kidneys, livers and brains of rats with sealer implants by ICP-MS
Following root canal treatment, sealers may contact periapical tissue. The purpose of this study was to evaluate the systemic toxic effects of epoxy resin-based sealers (AH Plus and Obtuseal). Inductively coupled plasma-mass spectrometry (ICP-MS) was used to measure levels of trace elements (berylli...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2017-03-01
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| Series: | Biotechnology & Biotechnological Equipment |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/13102818.2017.1282327 |
| _version_ | 1818187591794556928 |
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| author | Neslihan Simsek Levent Akinci Hilal Alan Orhan Gecör Ülkü Özan |
| author_facet | Neslihan Simsek Levent Akinci Hilal Alan Orhan Gecör Ülkü Özan |
| author_sort | Neslihan Simsek |
| collection | DOAJ |
| description | Following root canal treatment, sealers may contact periapical tissue. The purpose of this study was to evaluate the systemic toxic effects of epoxy resin-based sealers (AH Plus and Obtuseal). Inductively coupled plasma-mass spectrometry (ICP-MS) was used to measure levels of trace elements (beryllium, magnesium, aluminium, calcium, chromium, arsenic and lead) in the brain, kidney and liver of rats. Twenty sterilized polyethylene tubes were then filled with AH Plus and Obtuseal and implanted into the dorsal subcutaneous tissue of 10 rats; three unoperated animals were used as a control group. After 45 days, the rats were sacrificed by cervical dislocation following anaesthesia, and brains, kidneys and livers were removed from all experimental animals. ICP-MS analysis was used to determine levels of trace elements. Data were analysed using Kruskal–Wallis and Connover post hoc tests. No significant differences were found in aluminium and calcium levels, but brains, kidneys and livers showed significantly higher amounts of magnesium and chromium than the corresponding controls. In the kidney and liver samples, arsenic levels were found to be higher than in the control group. Lead was detected at higher levels only in liver samples from the AH Plus group. Beryllium was not detected in any organ. It was concluded that AH Plus and Obtuseal release minimal quantities of trace elements when in contact with subcutaneous tissue, and further studies are needed to understand the systemic effects of these materials. |
| first_indexed | 2024-12-11T23:13:28Z |
| format | Article |
| id | doaj.art-dbc0bfb87c6046adae2feb474ee2c35f |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-12-11T23:13:28Z |
| publishDate | 2017-03-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-dbc0bfb87c6046adae2feb474ee2c35f2022-12-22T00:46:38ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302017-03-0131239740210.1080/13102818.2017.12823271282327Determination of trace elements in kidneys, livers and brains of rats with sealer implants by ICP-MSNeslihan Simsek0Levent Akinci1Hilal Alan2Orhan Gecör3Ülkü Özan4Inonu UniversityInonu UniversityInonu UniversityInonu UniversityAbant İzzet Baysal UniversityFollowing root canal treatment, sealers may contact periapical tissue. The purpose of this study was to evaluate the systemic toxic effects of epoxy resin-based sealers (AH Plus and Obtuseal). Inductively coupled plasma-mass spectrometry (ICP-MS) was used to measure levels of trace elements (beryllium, magnesium, aluminium, calcium, chromium, arsenic and lead) in the brain, kidney and liver of rats. Twenty sterilized polyethylene tubes were then filled with AH Plus and Obtuseal and implanted into the dorsal subcutaneous tissue of 10 rats; three unoperated animals were used as a control group. After 45 days, the rats were sacrificed by cervical dislocation following anaesthesia, and brains, kidneys and livers were removed from all experimental animals. ICP-MS analysis was used to determine levels of trace elements. Data were analysed using Kruskal–Wallis and Connover post hoc tests. No significant differences were found in aluminium and calcium levels, but brains, kidneys and livers showed significantly higher amounts of magnesium and chromium than the corresponding controls. In the kidney and liver samples, arsenic levels were found to be higher than in the control group. Lead was detected at higher levels only in liver samples from the AH Plus group. Beryllium was not detected in any organ. It was concluded that AH Plus and Obtuseal release minimal quantities of trace elements when in contact with subcutaneous tissue, and further studies are needed to understand the systemic effects of these materials.http://dx.doi.org/10.1080/13102818.2017.1282327Endodonticsepoxy resin-based sealersICP-MStrace elements |
| spellingShingle | Neslihan Simsek Levent Akinci Hilal Alan Orhan Gecör Ülkü Özan Determination of trace elements in kidneys, livers and brains of rats with sealer implants by ICP-MS Biotechnology & Biotechnological Equipment Endodontics epoxy resin-based sealers ICP-MS trace elements |
| title | Determination of trace elements in kidneys, livers and brains of rats with sealer implants by ICP-MS |
| title_full | Determination of trace elements in kidneys, livers and brains of rats with sealer implants by ICP-MS |
| title_fullStr | Determination of trace elements in kidneys, livers and brains of rats with sealer implants by ICP-MS |
| title_full_unstemmed | Determination of trace elements in kidneys, livers and brains of rats with sealer implants by ICP-MS |
| title_short | Determination of trace elements in kidneys, livers and brains of rats with sealer implants by ICP-MS |
| title_sort | determination of trace elements in kidneys livers and brains of rats with sealer implants by icp ms |
| topic | Endodontics epoxy resin-based sealers ICP-MS trace elements |
| url | http://dx.doi.org/10.1080/13102818.2017.1282327 |
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