Designing Efficient Processes for Sustainable Bioethanol and Bio-Hydrogen Production from Grass Lawn Waste

The effect of thermal, acid and alkali pretreatment methods on biological hydrogen (BHP) and bioethanol production (BP) from grass lawn (GL) waste was investigated, under different process schemes. BHP from the whole pretreatment slurry of GL was performed through mixed microbial cultures in simulta...

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Main Author: Georgia Antonopoulou
Format: Article
Language:English
Published: MDPI AG 2020-06-01
Series:Molecules
Subjects:
Online Access:https://www.mdpi.com/1420-3049/25/12/2889
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author Georgia Antonopoulou
author_facet Georgia Antonopoulou
author_sort Georgia Antonopoulou
collection DOAJ
description The effect of thermal, acid and alkali pretreatment methods on biological hydrogen (BHP) and bioethanol production (BP) from grass lawn (GL) waste was investigated, under different process schemes. BHP from the whole pretreatment slurry of GL was performed through mixed microbial cultures in simultaneous saccharification and fermentation (SSF) mode, while BP was carried out through the C5yeast <i>Pichia stipitis</i>, in SSF mode. From these experiments, the best pretreatment conditions were determined and the efficiencies for each process were assessed and compared, when using either the whole pretreatment slurry or the separated fractions (solid and liquid), the separate hydrolysis and fermentation (SHF) or SSF mode, and especially for BP, the use of other yeasts such as <i>Pachysolen tannophilus</i> or <i>Saccharomyces cerevisiae</i>. The experimental results showed that pretreatment with 10 gH<sub>2</sub>SO<sub>4</sub>/100 g total solids (TS) was the optimum for both BHP and BP. Separation of solid and liquid pretreated fractions led to the highest BHP (270.1 mL H<sub>2</sub>/g TS, corresponding to 3.4 MJ/kg TS) and also BP (108.8 mg ethanol/g TS, corresponding to 2.9 MJ/kg TS) yields. The latter was achieved by using <i>P. stipitis</i> for the fermentation of the hydrolysate and <i>S. serevisiae</i> for the solid fraction fermentation, at SSF.
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spelling doaj.art-dbc207c29e574a53b1961b5e42fd58222023-11-20T04:45:49ZengMDPI AGMolecules1420-30492020-06-012512288910.3390/molecules25122889Designing Efficient Processes for Sustainable Bioethanol and Bio-Hydrogen Production from Grass Lawn WasteGeorgia Antonopoulou0Institute of Chemical Engineering Sciences, Stadiou, Platani, 26504 Patras, GreeceThe effect of thermal, acid and alkali pretreatment methods on biological hydrogen (BHP) and bioethanol production (BP) from grass lawn (GL) waste was investigated, under different process schemes. BHP from the whole pretreatment slurry of GL was performed through mixed microbial cultures in simultaneous saccharification and fermentation (SSF) mode, while BP was carried out through the C5yeast <i>Pichia stipitis</i>, in SSF mode. From these experiments, the best pretreatment conditions were determined and the efficiencies for each process were assessed and compared, when using either the whole pretreatment slurry or the separated fractions (solid and liquid), the separate hydrolysis and fermentation (SHF) or SSF mode, and especially for BP, the use of other yeasts such as <i>Pachysolen tannophilus</i> or <i>Saccharomyces cerevisiae</i>. The experimental results showed that pretreatment with 10 gH<sub>2</sub>SO<sub>4</sub>/100 g total solids (TS) was the optimum for both BHP and BP. Separation of solid and liquid pretreated fractions led to the highest BHP (270.1 mL H<sub>2</sub>/g TS, corresponding to 3.4 MJ/kg TS) and also BP (108.8 mg ethanol/g TS, corresponding to 2.9 MJ/kg TS) yields. The latter was achieved by using <i>P. stipitis</i> for the fermentation of the hydrolysate and <i>S. serevisiae</i> for the solid fraction fermentation, at SSF.https://www.mdpi.com/1420-3049/25/12/2889pretreatmentfermentationhydrolysisgrass lawnethanolhydrogen
spellingShingle Georgia Antonopoulou
Designing Efficient Processes for Sustainable Bioethanol and Bio-Hydrogen Production from Grass Lawn Waste
Molecules
pretreatment
fermentation
hydrolysis
grass lawn
ethanol
hydrogen
title Designing Efficient Processes for Sustainable Bioethanol and Bio-Hydrogen Production from Grass Lawn Waste
title_full Designing Efficient Processes for Sustainable Bioethanol and Bio-Hydrogen Production from Grass Lawn Waste
title_fullStr Designing Efficient Processes for Sustainable Bioethanol and Bio-Hydrogen Production from Grass Lawn Waste
title_full_unstemmed Designing Efficient Processes for Sustainable Bioethanol and Bio-Hydrogen Production from Grass Lawn Waste
title_short Designing Efficient Processes for Sustainable Bioethanol and Bio-Hydrogen Production from Grass Lawn Waste
title_sort designing efficient processes for sustainable bioethanol and bio hydrogen production from grass lawn waste
topic pretreatment
fermentation
hydrolysis
grass lawn
ethanol
hydrogen
url https://www.mdpi.com/1420-3049/25/12/2889
work_keys_str_mv AT georgiaantonopoulou designingefficientprocessesforsustainablebioethanolandbiohydrogenproductionfromgrasslawnwaste