Phenotypic effect of growth media on Arabidopsis thaliana root hair growth
Over the years, many different growth media have been used to grow Arabidopsis thaliana in vitro in petri dishes. For these media the nutrient composition may vary, sugars may or may not be added, the medium may or may not be buffered and there is a choice between different gelling agents. The magni...
| Auteurs principaux: | , |
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| Format: | Article |
| Langue: | English |
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Taylor & Francis Group
2022-12-01
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| Collection: | Plant Signaling & Behavior |
| Sujets: | |
| Accès en ligne: | http://dx.doi.org/10.1080/15592324.2022.2104002 |
| _version_ | 1827379202593128448 |
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| author | Naomi Claeijs Kris Vissenberg |
| author_facet | Naomi Claeijs Kris Vissenberg |
| author_sort | Naomi Claeijs |
| collection | DOAJ |
| description | Over the years, many different growth media have been used to grow Arabidopsis thaliana in vitro in petri dishes. For these media the nutrient composition may vary, sugars may or may not be added, the medium may or may not be buffered and there is a choice between different gelling agents. The magnitude of possible combinations of these variables obstructs easy comparison of seedling phenotypes grown on the different media. This is especially obvious when it concerns the study of root hairs that are extremely sensitive to changes in their environment. To demonstrate this effect, we have grown Arabidopsis thaliana wild-type seeds on 18 different combinations of growth media and quantified root hair development. Comparison of root hair length and the respective root hair profiles identified the media that result in the formation of the longest root hairs. On these favored media they elongate through tip growth at a constant growth rate until they reach their final length (around 0.6 mm) at a distance of ±4 mm from the root tip. |
| first_indexed | 2024-03-08T13:08:46Z |
| format | Article |
| id | doaj.art-dbc260b3fb9f450f9178c1ad0f65efff |
| institution | Directory Open Access Journal |
| issn | 1559-2316 1559-2324 |
| language | English |
| last_indexed | 2024-03-08T13:08:46Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Plant Signaling & Behavior |
| spelling | doaj.art-dbc260b3fb9f450f9178c1ad0f65efff2024-01-18T15:58:22ZengTaylor & Francis GroupPlant Signaling & Behavior1559-23161559-23242022-12-0117110.1080/15592324.2022.21040022104002Phenotypic effect of growth media on Arabidopsis thaliana root hair growthNaomi Claeijs0Kris Vissenberg1University of AntwerpUniversity of AntwerpOver the years, many different growth media have been used to grow Arabidopsis thaliana in vitro in petri dishes. For these media the nutrient composition may vary, sugars may or may not be added, the medium may or may not be buffered and there is a choice between different gelling agents. The magnitude of possible combinations of these variables obstructs easy comparison of seedling phenotypes grown on the different media. This is especially obvious when it concerns the study of root hairs that are extremely sensitive to changes in their environment. To demonstrate this effect, we have grown Arabidopsis thaliana wild-type seeds on 18 different combinations of growth media and quantified root hair development. Comparison of root hair length and the respective root hair profiles identified the media that result in the formation of the longest root hairs. On these favored media they elongate through tip growth at a constant growth rate until they reach their final length (around 0.6 mm) at a distance of ±4 mm from the root tip.http://dx.doi.org/10.1080/15592324.2022.2104002arabidopsis thalianagelling agentgrowth mediain vitro growthphenotyperoot hair development |
| spellingShingle | Naomi Claeijs Kris Vissenberg Phenotypic effect of growth media on Arabidopsis thaliana root hair growth Plant Signaling & Behavior arabidopsis thaliana gelling agent growth media in vitro growth phenotype root hair development |
| title | Phenotypic effect of growth media on Arabidopsis thaliana root hair growth |
| title_full | Phenotypic effect of growth media on Arabidopsis thaliana root hair growth |
| title_fullStr | Phenotypic effect of growth media on Arabidopsis thaliana root hair growth |
| title_full_unstemmed | Phenotypic effect of growth media on Arabidopsis thaliana root hair growth |
| title_short | Phenotypic effect of growth media on Arabidopsis thaliana root hair growth |
| title_sort | phenotypic effect of growth media on arabidopsis thaliana root hair growth |
| topic | arabidopsis thaliana gelling agent growth media in vitro growth phenotype root hair development |
| url | http://dx.doi.org/10.1080/15592324.2022.2104002 |
| work_keys_str_mv | AT naomiclaeijs phenotypiceffectofgrowthmediaonarabidopsisthalianaroothairgrowth AT krisvissenberg phenotypiceffectofgrowthmediaonarabidopsisthalianaroothairgrowth |