The Simultaneous Determination of Chlorpyrifos–Ethyl and –Methyl with a New Format of Fluorescence-Based Immunochromatographic Assay
The improper and excessive use in agriculture of chlorpyrifos–methyl (CPSM) and chlorpyrifos–ethyl (CPSE) may affect the health of human beings. Herein, a fluorescence-based immunochromatographic assay (FICA) was developed for the simultaneous determination of CPSM and CPSE. A monoclonal antibody (m...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/11/1006 |
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| author | Zi-Hong Xu Jia Liu Bin Li Jun-Kai Wang Xi Zeng Zi-Jian Chen Surat Hongsibsong Wei Huang Hong-Tao Lei Yuan-Ming Sun Zhen-Lin Xu |
| author_facet | Zi-Hong Xu Jia Liu Bin Li Jun-Kai Wang Xi Zeng Zi-Jian Chen Surat Hongsibsong Wei Huang Hong-Tao Lei Yuan-Ming Sun Zhen-Lin Xu |
| author_sort | Zi-Hong Xu |
| collection | DOAJ |
| description | The improper and excessive use in agriculture of chlorpyrifos–methyl (CPSM) and chlorpyrifos–ethyl (CPSE) may affect the health of human beings. Herein, a fluorescence-based immunochromatographic assay (FICA) was developed for the simultaneous determination of CPSM and CPSE. A monoclonal antibody (mAb) with equal recognition of CPSM and CPSE was generated by the careful designing of haptens and screening of hybridoma cells. Instead of labeling fluorescence with mAb, the probe was labeled with goat-anti-mouse IgG (GAM-IgG) and pre-incubated with mAb in the sample. The complex could compete with CPS by coating antigen in the test line. The new format of FICA used goat-anti-rabbit IgG (GAR-IgG) conjugated with rabbit IgG labeled with fluorescence microspheres as an independent quality control line (C line). The novel strategy significantly reduced nonspecific reactions and increased assay sensitivity. Under the optimal conditions, the proposed FICA showed a linear range of 0.015–64 mg/L and limit of detection (LOD) of 0.015 mg/L for both CPSE and CPSM. The average recoveries of CPS from spiked food samples by FICA were 82.0–110.0%. The accuracy was similar to the gas chromatography–tandem mass spectrometry (GC-MS/MS) results. The developed FICA was an ideal on-site tool for rapid screening of CPS residues in foods. |
| first_indexed | 2024-03-09T18:27:50Z |
| format | Article |
| id | doaj.art-dbc387c952094014bc1a616c7550f420 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T18:27:50Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-dbc387c952094014bc1a616c7550f4202023-11-24T07:48:26ZengMDPI AGBiosensors2079-63742022-11-011211100610.3390/bios12111006The Simultaneous Determination of Chlorpyrifos–Ethyl and –Methyl with a New Format of Fluorescence-Based Immunochromatographic AssayZi-Hong Xu0Jia Liu1Bin Li2Jun-Kai Wang3Xi Zeng4Zi-Jian Chen5Surat Hongsibsong6Wei Huang7Hong-Tao Lei8Yuan-Ming Sun9Zhen-Lin Xu10Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, ChinaGuangzhou Institute of Food Inspection, Guangzhou 510410, ChinaGuangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, ChinaGuangzhou Institute of Food Inspection, Guangzhou 510410, ChinaGuangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, ChinaSchool of Health Sciences Research, Research Institute for Health Science, Chiang Mai University, Chiang Mai 50200, ThailandGuangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, ChinaThe improper and excessive use in agriculture of chlorpyrifos–methyl (CPSM) and chlorpyrifos–ethyl (CPSE) may affect the health of human beings. Herein, a fluorescence-based immunochromatographic assay (FICA) was developed for the simultaneous determination of CPSM and CPSE. A monoclonal antibody (mAb) with equal recognition of CPSM and CPSE was generated by the careful designing of haptens and screening of hybridoma cells. Instead of labeling fluorescence with mAb, the probe was labeled with goat-anti-mouse IgG (GAM-IgG) and pre-incubated with mAb in the sample. The complex could compete with CPS by coating antigen in the test line. The new format of FICA used goat-anti-rabbit IgG (GAR-IgG) conjugated with rabbit IgG labeled with fluorescence microspheres as an independent quality control line (C line). The novel strategy significantly reduced nonspecific reactions and increased assay sensitivity. Under the optimal conditions, the proposed FICA showed a linear range of 0.015–64 mg/L and limit of detection (LOD) of 0.015 mg/L for both CPSE and CPSM. The average recoveries of CPS from spiked food samples by FICA were 82.0–110.0%. The accuracy was similar to the gas chromatography–tandem mass spectrometry (GC-MS/MS) results. The developed FICA was an ideal on-site tool for rapid screening of CPS residues in foods.https://www.mdpi.com/2079-6374/12/11/1006chlorpyrifoshapten designmonoclonal antibodyfluorescence-based immunochromatographic assayquantitative detection |
| spellingShingle | Zi-Hong Xu Jia Liu Bin Li Jun-Kai Wang Xi Zeng Zi-Jian Chen Surat Hongsibsong Wei Huang Hong-Tao Lei Yuan-Ming Sun Zhen-Lin Xu The Simultaneous Determination of Chlorpyrifos–Ethyl and –Methyl with a New Format of Fluorescence-Based Immunochromatographic Assay Biosensors chlorpyrifos hapten design monoclonal antibody fluorescence-based immunochromatographic assay quantitative detection |
| title | The Simultaneous Determination of Chlorpyrifos–Ethyl and –Methyl with a New Format of Fluorescence-Based Immunochromatographic Assay |
| title_full | The Simultaneous Determination of Chlorpyrifos–Ethyl and –Methyl with a New Format of Fluorescence-Based Immunochromatographic Assay |
| title_fullStr | The Simultaneous Determination of Chlorpyrifos–Ethyl and –Methyl with a New Format of Fluorescence-Based Immunochromatographic Assay |
| title_full_unstemmed | The Simultaneous Determination of Chlorpyrifos–Ethyl and –Methyl with a New Format of Fluorescence-Based Immunochromatographic Assay |
| title_short | The Simultaneous Determination of Chlorpyrifos–Ethyl and –Methyl with a New Format of Fluorescence-Based Immunochromatographic Assay |
| title_sort | simultaneous determination of chlorpyrifos ethyl and methyl with a new format of fluorescence based immunochromatographic assay |
| topic | chlorpyrifos hapten design monoclonal antibody fluorescence-based immunochromatographic assay quantitative detection |
| url | https://www.mdpi.com/2079-6374/12/11/1006 |
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