IgE and IgG4 Epitopes of Dermatophagoides and Blomia Allergens before and after Sublingual Immunotherapy
Sublingual immunotherapy (SLIT) is used worldwide to treat house dust mites (HDM) allergy. Epitope specific immunotherapy with peptide vaccines is used far less, but it is of great interest in the treatment of allergic reactions, as it precludes the drawbacks of allergen extracts. The ideal peptide...
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MDPI AG
2023-02-01
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| Series: | International Journal of Molecular Sciences |
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| author | Daniele Danella Figo Priscilla Rios Cordeiro Macedo Gabriele Gadermaier Cesar Remuzgo Fábio Fernandes Morato Castro Jorge Kalil Clovis Eduardo Santos Galvão Keity Souza Santos |
| author_facet | Daniele Danella Figo Priscilla Rios Cordeiro Macedo Gabriele Gadermaier Cesar Remuzgo Fábio Fernandes Morato Castro Jorge Kalil Clovis Eduardo Santos Galvão Keity Souza Santos |
| author_sort | Daniele Danella Figo |
| collection | DOAJ |
| description | Sublingual immunotherapy (SLIT) is used worldwide to treat house dust mites (HDM) allergy. Epitope specific immunotherapy with peptide vaccines is used far less, but it is of great interest in the treatment of allergic reactions, as it precludes the drawbacks of allergen extracts. The ideal peptide candidates would bind to IgG, blocking IgE-binding. To better elucidate IgE and IgG4 epitope profiles during SLIT, sequences of main allergens, Der p 1, 2, 5, 7, 10, 23 and Blo t 5, 6, 12, 13, were included in a 15-mer peptide microarray and tested against pooled sera from 10 patients pre- and post-1-year SLIT. All allergens were recognized to some extent by at least one antibody isotype and peptide diversity was higher post-1-year SLIT for both antibodies. IgE recognition diversity varied among allergens and timepoints without a clear tendency. Der p 10, a minor allergen in temperate regions, was the molecule with more IgE-peptides and might be a major allergen in populations highly exposed to helminths and cockroaches, such as Brazil. SLIT-induced IgG4 epitopes were directed against several, but not all, IgE-binding regions. We selected a set of peptides that recognized only IgG4 or were able to induce increased ratios of IgG4:IgE after one year of treatment and might be potential targets for vaccines. |
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| spelling | doaj.art-dbc4630390b947f1b5103fa01f5a10ca2023-11-16T21:10:34ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-02-01244417310.3390/ijms24044173IgE and IgG4 Epitopes of Dermatophagoides and Blomia Allergens before and after Sublingual ImmunotherapyDaniele Danella Figo0Priscilla Rios Cordeiro Macedo1Gabriele Gadermaier2Cesar Remuzgo3Fábio Fernandes Morato Castro4Jorge Kalil5Clovis Eduardo Santos Galvão6Keity Souza Santos7LIM-19, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo 05403-900, BrazilDepartamento de Clinica Medica, Disciplina de Imunologia Clinica e Alergia, Faculdade de Medicina da Universidade de Sao Paulo, Sao Paulo 01246-903, BrazilDepartment of Biosciences and Medical Biology, Paris Lodron University Salzburg, 5020 Salzburg, AustriaLaboratorio de Imunologia, INCOR, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo 05403-900, BrazilDepartamento de Clinica Medica, Disciplina de Imunologia Clinica e Alergia, Faculdade de Medicina da Universidade de Sao Paulo, Sao Paulo 01246-903, BrazilLIM-19, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo 05403-900, BrazilDepartamento de Clinica Medica, Disciplina de Imunologia Clinica e Alergia, Faculdade de Medicina da Universidade de Sao Paulo, Sao Paulo 01246-903, BrazilLIM-19, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo 05403-900, BrazilSublingual immunotherapy (SLIT) is used worldwide to treat house dust mites (HDM) allergy. Epitope specific immunotherapy with peptide vaccines is used far less, but it is of great interest in the treatment of allergic reactions, as it precludes the drawbacks of allergen extracts. The ideal peptide candidates would bind to IgG, blocking IgE-binding. To better elucidate IgE and IgG4 epitope profiles during SLIT, sequences of main allergens, Der p 1, 2, 5, 7, 10, 23 and Blo t 5, 6, 12, 13, were included in a 15-mer peptide microarray and tested against pooled sera from 10 patients pre- and post-1-year SLIT. All allergens were recognized to some extent by at least one antibody isotype and peptide diversity was higher post-1-year SLIT for both antibodies. IgE recognition diversity varied among allergens and timepoints without a clear tendency. Der p 10, a minor allergen in temperate regions, was the molecule with more IgE-peptides and might be a major allergen in populations highly exposed to helminths and cockroaches, such as Brazil. SLIT-induced IgG4 epitopes were directed against several, but not all, IgE-binding regions. We selected a set of peptides that recognized only IgG4 or were able to induce increased ratios of IgG4:IgE after one year of treatment and might be potential targets for vaccines.https://www.mdpi.com/1422-0067/24/4/4173IgEIgGhouse dust mitesimmunotherapyepitopes |
| spellingShingle | Daniele Danella Figo Priscilla Rios Cordeiro Macedo Gabriele Gadermaier Cesar Remuzgo Fábio Fernandes Morato Castro Jorge Kalil Clovis Eduardo Santos Galvão Keity Souza Santos IgE and IgG4 Epitopes of Dermatophagoides and Blomia Allergens before and after Sublingual Immunotherapy International Journal of Molecular Sciences IgE IgG house dust mites immunotherapy epitopes |
| title | IgE and IgG4 Epitopes of Dermatophagoides and Blomia Allergens before and after Sublingual Immunotherapy |
| title_full | IgE and IgG4 Epitopes of Dermatophagoides and Blomia Allergens before and after Sublingual Immunotherapy |
| title_fullStr | IgE and IgG4 Epitopes of Dermatophagoides and Blomia Allergens before and after Sublingual Immunotherapy |
| title_full_unstemmed | IgE and IgG4 Epitopes of Dermatophagoides and Blomia Allergens before and after Sublingual Immunotherapy |
| title_short | IgE and IgG4 Epitopes of Dermatophagoides and Blomia Allergens before and after Sublingual Immunotherapy |
| title_sort | ige and igg4 epitopes of dermatophagoides and blomia allergens before and after sublingual immunotherapy |
| topic | IgE IgG house dust mites immunotherapy epitopes |
| url | https://www.mdpi.com/1422-0067/24/4/4173 |
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